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Exome Sequencing and Linkage Analysis Identified Tenascin-C (TNC) as a Novel Causative Gene in Nonsyndromic Hearing Loss
Zhao, Yali1; Zhao, Feifan1; Zong, Liang1; Zhang, Peng2; Guan, Liping2; Zhang, Jianguo2,3; Wang, Dayong1; Wang, Jing4; Chai, Wei5; Lan, Lan1; Li, Qian1; Han, Bing1; Yang, Ling6; Jin, Xin2,7; Yang, Weiyan1; Hu, Xiaoxiang8; Wang, Xiaoning9; Li, Ning8; Li, Yingrui; Petit, Christine10,11; Wang, Jun2,12; Yang, Huanming; Wang, Jian; Wang, Qiuju1; Wang, QJ (reprint author), Chinese Peoples Liberat Army Gen Hosp, Inst Otolaryngol, Dept Otorhinolaryngol Head & Neck Surg, Beijing, Peoples R China.
2013
Source PublicationPLOS ONE
ISSN1932-6203
Subtype期刊论文
Volume8Issue:7
Contribution Rank4
AbstractIn this study, a five-generation Chinese family (family F013) with progressive autosomal dominant hearing loss was mapped to a critical region spanning 28.54 Mb on chromosome 9q31.3-q34.3 by linkage analysis, which was a novel DFNA locus, assigned as DFNA56. In this interval, there were 398 annotated genes. Then, whole exome sequencing was applied in three patients and one normal individual from this family. Six single nucleotide variants and two indels were found co-segregated with the phenotypes. Then using mass spectrum (Sequenom, Inc.) to rank the eight sites, we found only the TNC gene be co-segregated with hearing loss in 53 subjects of F013. And this missense mutation (c.5317G>A, p.V1773M) of TNC located exactly in the critical linked interval. Further screening to the coding region of this gene in 587 subjects with nonsyndromic hearing loss (NSHL) found a second missense mutation, c.5368A>T (p. T1796S), co-segregating with phenotype in the other family. These two mutations located in the conserved region of TNC and were absent in the 387 normal hearing individuals of matched geographical ancestry. Functional effects of the two mutations were predicted using SIFT and both mutations were deleterious. All these results supported that TNC may be the causal gene for the hearing loss inherited in these families. TNC encodes tenascin-C, a member of the extracellular matrix (ECM), is present in the basilar membrane (BM), and the osseous spiral lamina of the cochlea. It plays an important role in cochlear development. The up-regulated expression of TNC gene in tissue repair and neural regeneration was seen in human and zebrafish, and in sensory receptor recovery in the vestibular organ after ototoxic injury in birds. Then the absence of normal tenascin-C was supposed to cause irreversible injuries in cochlea and caused hearing loss.
Subject AreaPhysiological Psychology/biological Psychology
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Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China [81120108009] ; National Natural Science Foundation of Youth Science Foundation [81100719]
Project Intro.This work was supported by the grants of the National Natural Science Foundation of China, Major Project, No. 81120108009 and National Natural Science Foundation of Youth Science Foundation No.81100719. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
WOS IDWOS:000323114200039
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Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/10629
Collection中国科学院心理健康重点实验室
Corresponding AuthorWang, QJ (reprint author), Chinese Peoples Liberat Army Gen Hosp, Inst Otolaryngol, Dept Otorhinolaryngol Head & Neck Surg, Beijing, Peoples R China.
Affiliation1.Chinese Peoples Liberat Army Gen Hosp, Inst Otolaryngol, Dept Otorhinolaryngol Head & Neck Surg, Beijing, Peoples R China
2.BGI Shenzhen, Shenzhen, Peoples R China
3.Fudan Univ, T Life Res Ctr, Shanghai 200433, Peoples R China
4.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
5.Chinese Peoples Liberat Army Gen Hosp, Dept Orthopaed Surg, Beijing, Peoples R China
6.BGI Tianjin, Tianjin, Peoples R China
7.S China Univ Technol, Sch Biosci & Biotechnol, Guangzhou, Guangdong, Peoples R China
8.China Agr Univ, Coll Biol Sci, State Key Lab AgroBiotechnol, Beijing 100094, Peoples R China
9.Chinese Peoples Liberat Army Gen Hosp, Inst Life Sci, Beijing, Peoples R China
10.Coll France, F-75231 Paris, France
11.Inst Pasteur, Paris, France
12.Univ Copenhagen, Dept Biol, Copenhagen, Denmark
Recommended Citation
GB/T 7714
Zhao, Yali,Zhao, Feifan,Zong, Liang,et al. Exome Sequencing and Linkage Analysis Identified Tenascin-C (TNC) as a Novel Causative Gene in Nonsyndromic Hearing Loss[J]. PLOS ONE,2013,8(7).
APA Zhao, Yali.,Zhao, Feifan.,Zong, Liang.,Zhang, Peng.,Guan, Liping.,...&Wang, QJ .(2013).Exome Sequencing and Linkage Analysis Identified Tenascin-C (TNC) as a Novel Causative Gene in Nonsyndromic Hearing Loss.PLOS ONE,8(7).
MLA Zhao, Yali,et al."Exome Sequencing and Linkage Analysis Identified Tenascin-C (TNC) as a Novel Causative Gene in Nonsyndromic Hearing Loss".PLOS ONE 8.7(2013).
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