PSYCH OpenIR  > 中国科学院心理健康重点实验室
Expression of Ephrin Receptors and Ligands in Postmortem Brains of HIV-Infected Subjects With and Without Cognitive Impairment
Yuferov, Vadim; Ho, Ann; Morgello, Susan; Yang, Yaning; Ott, Jurg; Kreek, Mary Jeanne; Yuferov, V (reprint author), Rockefeller Univ, 1230 York Ave, New York, NY 10021 USA.
2013
Source PublicationJOURNAL OF NEUROIMMUNE PHARMACOLOGY
ISSN1674-2788
Subtype期刊论文
Volume8Issue:1Pages:333-344
Contribution Rank4
AbstractDespite the successes of combination antiretroviral therapy, HIV-associated neurocognitive disorders persist in many infected individuals. Earlier studies showed that neurocognitive impairment was associated with glutamate toxicity and synaptodendritic damage. We examined alterations in expression of four ephrin genes that are involved in synapse formation and recruitment of glutamate receptors to synapses, in the caudate and anterior cingulate in postmortem brain of cognitively characterized HIV-infected subjects, along with expression of neuronal and astroglial/macroglial markers. Postmortem tissues of HIV-infected and control subjects were obtained from the Manhattan HIV Brain Bank. HIV-infected subjects underwent neurocognitive assessment prior to death. Quantification of mRNA of genes of chemokine receptors and chemokines (CCR5, CXCR4, CCL2), astroglial/microglial markers (GFAP, CD163, CD68), the neuronal marker SNAP25, ephrin receptors EPHA4 and EPHB2, and ephrin ligands EFNB1 and EFNB2 was performed using SYBR Green RT-PCR. Proinflammatory chemokine and glial/macrophage mRNA levels in both regions were significantly greater in HIV+ than in HIV- subjects. Levels of EPHA4 and EFNB2 mRNA in the caudate, and EPHB2 mRNA in anterior cingulate were significantly lower in HIV+ subjects (p < 0.002, p < 0.02, p < 0.05, respectively). These transcripts also showed correlations with immune status and cognitive function within the HIV-infected group. Decreased levels of EFNB2 mRNA in the caudate correlated with lower CD4 counts (P < 0.05). Cognitive associations were limited to the cingulate, where decreased levels of EPHB2 mRNA were associated with better global cognitive status. Decreased cingulate expression of EPHB2 may represent a compensatory mechanism minimizing excitotoxic injury in the face of chronic inflammation.
KeywordHIV dementia Human postmortem brain Ephrin receptors and ligands Chemokine receptors and ligands Neurocognitive impairment
Subject AreaCognitive Neuroscience
URL查看原文
Indexed BySCI
Language英语
Funding OrganizationNIH [NIDA-P60-05130, NIMH-U01-MH083501]
Project Intro.This work was supported by NIH NIDA-P60-05130 (MJK), NIMH-U01-MH083501 (SM). We thank Allie Sperry for help in performance of RT-PCR assays, and the patients and staff of the Manhattan HIV Brain Bank. We acknowledge the help of Dr. Keiichi Niikura, Adam J. Brownstein, and Molly Deutsch-Feldman for the art work of the diagram.
WOS IDWOS:000315441900031
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/11512
Collection中国科学院心理健康重点实验室
Corresponding AuthorYuferov, V (reprint author), Rockefeller Univ, 1230 York Ave, New York, NY 10021 USA.
Recommended Citation
GB/T 7714
Yuferov, Vadim,Ho, Ann,Morgello, Susan,et al. Expression of Ephrin Receptors and Ligands in Postmortem Brains of HIV-Infected Subjects With and Without Cognitive Impairment[J]. JOURNAL OF NEUROIMMUNE PHARMACOLOGY,2013,8(1):333-344.
APA Yuferov, Vadim.,Ho, Ann.,Morgello, Susan.,Yang, Yaning.,Ott, Jurg.,...&Yuferov, V .(2013).Expression of Ephrin Receptors and Ligands in Postmortem Brains of HIV-Infected Subjects With and Without Cognitive Impairment.JOURNAL OF NEUROIMMUNE PHARMACOLOGY,8(1),333-344.
MLA Yuferov, Vadim,et al."Expression of Ephrin Receptors and Ligands in Postmortem Brains of HIV-Infected Subjects With and Without Cognitive Impairment".JOURNAL OF NEUROIMMUNE PHARMACOLOGY 8.1(2013):333-344.
Files in This Item:
File Name/Size DocType Version Access License
WOS000315441900031.p(349KB) 限制开放Apache LicenseView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yuferov, Vadim]'s Articles
[Ho, Ann]'s Articles
[Morgello, Susan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yuferov, Vadim]'s Articles
[Ho, Ann]'s Articles
[Morgello, Susan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yuferov, Vadim]'s Articles
[Ho, Ann]'s Articles
[Morgello, Susan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: WOS000315441900031.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.