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Identification of a novel nanoRNase in Bartonella
Liu, Ma Feng1,2; Cescau, Sandra3; Mechold, Undine3; Wang, Jing4; Cohen, Dorit5; Danchin, Antoine6; Boulouis, Henri-Jean1; Biville, Francis1,3; Biville, F (reprint author), UMR BIPAR INRA AFSSA ENVA, 23 Ave Gen Gaulle, F-94700 Maisons Alfort, France.
2012-04-01
发表期刊MICROBIOLOGY-SGM
ISSN1350-0872
文章类型Article
卷号158页码:886-895
产权排序4
摘要In Escherichia coli, only one essential oligoribonuclease (Orn) can degrade oligoribonucleotides of five residues and shorter in length (nanoRNA). In Bacillus subtilis, NrnA and NrnB, which do not show any sequence similarity to Orn, have been identified as functional analogues of Orn. Sequence comparisons did not identify orn, nrnA or nrnB homologues in the genomes of the Chlamydia/Cyanobacteria and Alphaproteobacteria family members. Screening a genomic library from Bartonella birtlesii, a member of the Alphaproteobacteria, for genes that can complement a conditional orn mutant. in E. coli, we identified BA0969 (NrnC) as a functional analogue of Orn. NrnC is highly conserved (more than 80% identity) in the Bartonella genomes sequenced to date. Biochemical characterization showed that this protein exhibits oligo RNA degradation activity (nanoRNase activity). Like Orn from E. coli, NrnC is inhibited by micromolar amounts of 3'-phosphoadenosine 5'-phosphate in vitro. NrnC homologues are widely present in genomes of Alphaproteobacteria. Knock down of nrnC decreases the growth ability of Bartonella henselae, demonstrating the importance of nanoRNase activity in this bacterium.
学科领域Physiological Psychology/biological Psychology
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收录类别SCI
语种英语
项目资助者Pasteur Institute ; Centre National de la Recherche Scientifique [URA2172] ; Agence Nationale pour la Recherche [ANR-06-MIME-019-01] ; TARPOL [KBBE-212894] ; China Scholarship Council
项目简介We thank Dr D. Gillaspie and Dr B. E. Anderson (University of South Florida) for the generous gift of plasmids pNS2Amp and pNS2Trc. This work was supported by the Pasteur Institute, the Centre National de la Recherche Scientifique (URA2172), the Agence Nationale pour la Recherche (ANR-06-MIME-019-01) and TARPOL (grant KBBE-212894). M. F. L. was supported by the China Scholarship Council.
WOS记录号WOS:000303633200003
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被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/12848
专题中国科学院心理健康重点实验室
通讯作者Biville, F (reprint author), UMR BIPAR INRA AFSSA ENVA, 23 Ave Gen Gaulle, F-94700 Maisons Alfort, France.
作者单位1.UMR BIPAR INRA AFSSA ENVA, F-94700 Maisons Alfort, France
2.Jilin Univ, Inst Zoonosis, Minist Educ, Key Lab Zoonosis, Changchun 130062, Peoples R China
3.Inst Pasteur, F-75015 Paris, France
4.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
5.Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
6.AMAbiotics SAS, F-91000 Evry, France
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Liu, Ma Feng,Cescau, Sandra,Mechold, Undine,et al. Identification of a novel nanoRNase in Bartonella[J]. MICROBIOLOGY-SGM,2012,158:886-895.
APA Liu, Ma Feng.,Cescau, Sandra.,Mechold, Undine.,Wang, Jing.,Cohen, Dorit.,...&Biville, F .(2012).Identification of a novel nanoRNase in Bartonella.MICROBIOLOGY-SGM,158,886-895.
MLA Liu, Ma Feng,et al."Identification of a novel nanoRNase in Bartonella".MICROBIOLOGY-SGM 158(2012):886-895.
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