PSYCH OpenIR  > 中国科学院心理健康重点实验室
Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate
Yan, Hai-Qin1,2; Chang, Su-Hua3; Tian, Zhe-Xian1; Zhang, Le1; Sun, Yi-Cheng1,4,5; Li, Yan1; Wang, Jing3; Wang, Yi-Ping1; Yan, HQ (reprint author), Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China.
2011-05-18
Source PublicationPLOS ONE
ISSN1932-6203
SubtypeArticle
Volume6Issue:5
Contribution Rank3
AbstractGlyphosate is a non-selective broad-spectrum herbicide that inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS, also designated as AroA), a key enzyme in the aromatic amino acid biosynthesis pathway in microorganisms and plants. Previously, we reported that a novel AroA (PpAroA1) from Pseudomonas putida had high tolerance to glyphosate, with little homology to class I or class II glyphosate-tolerant AroA. In this study, the coding sequence of PpAroA1 was optimized for tobacco. For maturation of the enzyme in chloroplast, a chloroplast transit peptide coding sequence was fused in frame with the optimized aroA gene (PparoA1(optimized)) at the 59 end. The PparoA1(optimized) gene was introduced into the tobacco (Nicotiana tabacum L. cv. W38) genome via Agrobacterium-mediated transformation. The transformed explants were first screened in shoot induction medium containing kanamycin. Then glyphosate tolerance was assayed in putative transgenic plants and its T-1 progeny. Our results show that the PpAroA1 from Pseudomonas putida can efficiently confer tobacco plants with high glyphosate tolerance. Transgenic tobacco overexpressing the PparoA1(optimized) gene exhibit high tolerance to glyphosate, which suggest that the novel PpAroA1 is a new and good candidate applied in transgenic crops with glyphosate tolerance in future.
Subject AreaPhysiological Psychology/biological Psychology
URL查看原文
Indexed BySCI
Language英语
Funding OrganizationNational Transgenic Research and Industrialization Special Foundation of China [2009ZX08009-057B, 2008ZX08009-003-002] ; Ministry of Science and Technology of China [2007AA021307]
Project Intro.This research was supported by grants from the National Transgenic Research and Industrialization Special Foundation of China No. 2009ZX08009-057B, No. 2008ZX08009-003-002, and the Hi-Tech Research and Development "863" Program No. 2007AA021307 from the Ministry of Science and Technology of China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
WOS IDWOS:000290720200029
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/12881
Collection中国科学院心理健康重点实验室
Corresponding AuthorYan, HQ (reprint author), Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China.
Affiliation1.Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China
2.Bengbu Med Coll, Dept Histol & Embryol, Bengbu Anhui, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
4.Chinese Acad Med Sci, Inst Pathogen Biol, Beijing 100037, Peoples R China
5.Peking Union Med Coll, Beijing 100021, Peoples R China
Recommended Citation
GB/T 7714
Yan, Hai-Qin,Chang, Su-Hua,Tian, Zhe-Xian,et al. Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate[J]. PLOS ONE,2011,6(5).
APA Yan, Hai-Qin.,Chang, Su-Hua.,Tian, Zhe-Xian.,Zhang, Le.,Sun, Yi-Cheng.,...&Yan, HQ .(2011).Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate.PLOS ONE,6(5).
MLA Yan, Hai-Qin,et al."Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate".PLOS ONE 6.5(2011).
Files in This Item:
File Name/Size DocType Version Access License
WOS_000290720200029.(458KB) 限制开放Apache LicenseView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yan, Hai-Qin]'s Articles
[Chang, Su-Hua]'s Articles
[Tian, Zhe-Xian]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yan, Hai-Qin]'s Articles
[Chang, Su-Hua]'s Articles
[Tian, Zhe-Xian]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yan, Hai-Qin]'s Articles
[Chang, Su-Hua]'s Articles
[Tian, Zhe-Xian]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: WOS_000290720200029.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.