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Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate
Yan, Hai-Qin1,2; Chang, Su-Hua3; Tian, Zhe-Xian1; Zhang, Le1; Sun, Yi-Cheng1,4,5; Li, Yan1; Wang, Jing3; Wang, Yi-Ping1; Yan, HQ (reprint author), Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China.
2011-05-18
发表期刊PLOS ONE
ISSN1932-6203
文章类型Article
卷号6期号:5
产权排序3
摘要Glyphosate is a non-selective broad-spectrum herbicide that inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS, also designated as AroA), a key enzyme in the aromatic amino acid biosynthesis pathway in microorganisms and plants. Previously, we reported that a novel AroA (PpAroA1) from Pseudomonas putida had high tolerance to glyphosate, with little homology to class I or class II glyphosate-tolerant AroA. In this study, the coding sequence of PpAroA1 was optimized for tobacco. For maturation of the enzyme in chloroplast, a chloroplast transit peptide coding sequence was fused in frame with the optimized aroA gene (PparoA1(optimized)) at the 59 end. The PparoA1(optimized) gene was introduced into the tobacco (Nicotiana tabacum L. cv. W38) genome via Agrobacterium-mediated transformation. The transformed explants were first screened in shoot induction medium containing kanamycin. Then glyphosate tolerance was assayed in putative transgenic plants and its T-1 progeny. Our results show that the PpAroA1 from Pseudomonas putida can efficiently confer tobacco plants with high glyphosate tolerance. Transgenic tobacco overexpressing the PparoA1(optimized) gene exhibit high tolerance to glyphosate, which suggest that the novel PpAroA1 is a new and good candidate applied in transgenic crops with glyphosate tolerance in future.
学科领域Physiological Psychology/biological Psychology
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收录类别SCI
语种英语
项目资助者National Transgenic Research and Industrialization Special Foundation of China [2009ZX08009-057B, 2008ZX08009-003-002] ; Ministry of Science and Technology of China [2007AA021307]
项目简介This research was supported by grants from the National Transgenic Research and Industrialization Special Foundation of China No. 2009ZX08009-057B, No. 2008ZX08009-003-002, and the Hi-Tech Research and Development "863" Program No. 2007AA021307 from the Ministry of Science and Technology of China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
WOS记录号WOS:000290720200029
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文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/12881
专题中国科学院心理健康重点实验室
通讯作者Yan, HQ (reprint author), Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China.
作者单位1.Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China
2.Bengbu Med Coll, Dept Histol & Embryol, Bengbu Anhui, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
4.Chinese Acad Med Sci, Inst Pathogen Biol, Beijing 100037, Peoples R China
5.Peking Union Med Coll, Beijing 100021, Peoples R China
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Yan, Hai-Qin,Chang, Su-Hua,Tian, Zhe-Xian,et al. Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate[J]. PLOS ONE,2011,6(5).
APA Yan, Hai-Qin.,Chang, Su-Hua.,Tian, Zhe-Xian.,Zhang, Le.,Sun, Yi-Cheng.,...&Yan, HQ .(2011).Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate.PLOS ONE,6(5).
MLA Yan, Hai-Qin,et al."Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate".PLOS ONE 6.5(2011).
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