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DNA damage and S phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK 293)
Yang, Qian1; He, Xiaoyun2; Li, Xiaohong1; Xu, Wentao1; Luo, Yunbo1; Yang, Xuan2; Wang, Yan2; Li, Yingcong3; Huang, Kunlun1
2014-07-01
Source PublicationMUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
ISSN0027-5107
SubtypeArticle
Volume765Issue:0Pages:22-31
AbstractOchratoxin A (OTA) is a ubiquitous mycotoxin with potential nephrotoxic, hepatotoxic and immunotoxic effects. The mechanisms underlying the nephrotoxicity of OTA remain obscure. To investigate DNA damage and the changes of the cell cycle distribution induced by OTA, human embryonic kidney cells (HEK 293 cells) were incubated with various concentrations of OTA for 24 h in vitro. The results indicated that OTA treatment led to the production of reactive oxygen species (ROS) and to a decrease of the mitochondrial membrane potential (Delta psi(m).). OTA-induced DNA damage in HEK 293 cells was evidenced by DNA comet tails formation and increased expression of gamma-H2AX. In addition, OTA could induce cell cycle arrest at the S phase in HEK 293 cells. The expression of key cell cycle regulatory factors that were critical to the S phase, including cyclin A2, cyclin El, and CDK2, were further detected. The expression of cyclin A2, cyclin El, and CDK2 were significantly decreased by OTA treatment at both the mRNA and protein levels. The apoptosis of HEK 293 cells after OTA treatment was observed using Hoechst 33342 staining. The results confirmed that OTA did induce apoptosis in HEK 293 cells. In conclusion, our results provided new insights into the molecular mechanisms by which OTA might promote nephrotoxicity. (C) 2014 Elsevier B.V. All rights reserved.
KeywordOchratoxin a Cell Cycle Arrest Dna Damage Hek 293 Cells
Indexed BySCI
Language英语
WOS IDWOS:000338610700004
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Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/13489
Collection健康与遗传心理学研究室
Affiliation1.China Agr Univ, Coll Food Sci & Nutr Engn, Lab Food Safety & Mol Biol, Beijing 100083, Peoples R China
2.Supervis Inspect & Testing Ctr Genetically Modifi, Minist Agr, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Yang, Qian,He, Xiaoyun,Li, Xiaohong,et al. DNA damage and S phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK 293)[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2014,765(0):22-31.
APA Yang, Qian.,He, Xiaoyun.,Li, Xiaohong.,Xu, Wentao.,Luo, Yunbo.,...&Huang, Kunlun.(2014).DNA damage and S phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK 293).MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,765(0),22-31.
MLA Yang, Qian,et al."DNA damage and S phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK 293)".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 765.0(2014):22-31.
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