| DNA damage and S phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK 293) | |
Yang, Qian1; He, Xiaoyun2; Li, Xiaohong1; Xu, Wentao1; Luo, Yunbo1; Yang, Xuan2; Wang, Yan2; Li, Yingcong3 ; Huang, Kunlun1
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| 摘要 | Ochratoxin A (OTA) is a ubiquitous mycotoxin with potential nephrotoxic, hepatotoxic and immunotoxic effects. The mechanisms underlying the nephrotoxicity of OTA remain obscure. To investigate DNA damage and the changes of the cell cycle distribution induced by OTA, human embryonic kidney cells (HEK 293 cells) were incubated with various concentrations of OTA for 24 h in vitro. The results indicated that OTA treatment led to the production of reactive oxygen species (ROS) and to a decrease of the mitochondrial membrane potential (Delta psi(m).). OTA-induced DNA damage in HEK 293 cells was evidenced by DNA comet tails formation and increased expression of gamma-H2AX. In addition, OTA could induce cell cycle arrest at the S phase in HEK 293 cells. The expression of key cell cycle regulatory factors that were critical to the S phase, including cyclin A2, cyclin El, and CDK2, were further detected. The expression of cyclin A2, cyclin El, and CDK2 were significantly decreased by OTA treatment at both the mRNA and protein levels. The apoptosis of HEK 293 cells after OTA treatment was observed using Hoechst 33342 staining. The results confirmed that OTA did induce apoptosis in HEK 293 cells. In conclusion, our results provided new insights into the molecular mechanisms by which OTA might promote nephrotoxicity. (C) 2014 Elsevier B.V. All rights reserved. |
| 关键词 | Ochratoxin a Cell Cycle Arrest Dna Damage Hek 293 Cells |
| 2014-07-01 | |
| 语种 | 英语 |
| 发表期刊 | MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
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| ISSN | 0027-5107 |
| 卷号 | 765期号:0页码:22-31 |
| 期刊论文类型 | Article |
| 收录类别 | SCI |
| WOS记录号 | WOS:000338610700004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.psych.ac.cn/handle/311026/13489 |
| 专题 | 健康与遗传心理学研究室 |
| 作者单位 | 1.China Agr Univ, Coll Food Sci & Nutr Engn, Lab Food Safety & Mol Biol, Beijing 100083, Peoples R China 2.Supervis Inspect & Testing Ctr Genetically Modifi, Minist Agr, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Inst Psychol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Qian,He, Xiaoyun,Li, Xiaohong,et al. DNA damage and S phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK 293)[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2014,765(0):22-31. |
| APA | Yang, Qian.,He, Xiaoyun.,Li, Xiaohong.,Xu, Wentao.,Luo, Yunbo.,...&Huang, Kunlun.(2014).DNA damage and S phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK 293).MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,765(0),22-31. |
| MLA | Yang, Qian,et al."DNA damage and S phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK 293)".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 765.0(2014):22-31. |
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| WOS_000338610700004.（1782KB） | 期刊论文 | 出版稿 | 暂不开放 | CC BY-NC-SA | 请求全文 | |
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