Superoxide Anion Regulates the Mitochondrial Free Ca2+ Through Uncoupling Proteins

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	Superoxide Anion Regulates the Mitochondrial Free Ca2+ Through Uncoupling Proteins
	Wu, Zhaofei 1,2; Zhang, Jie3 ; Zhao, Baolu 1,2,4,5
摘要	Mitochondrial dysfunction, which is closely related to intracellular calcium overload and excessive free radicals, is an important cause of Alzheimer's disease (AD). However, molecular mechanisms of the mitochondrial Ca2+ disregulation induced by oxidative stress in AD are still obscure. In an effort to gain a further understanding of this problem, we investigated the effects of superoxide anion, a primary free radical, on the expression of uncoupling proteins (UCPs) and the mitochondrial free Ca2+ levels in the neuroblastoma SH-SY5Y cell line (neo) and stably expressed wild-type human APP(APP) and APP-Swedish mutation (APPsw) SH-SY5Y cells. It was found that UCP2 and UCP4 protein levels were upregulated in neo but downregulated in APP and APPsw cells by the superoxide anion. Our results show that the superoxide anion can regulate protein levels of UCP2 and UCP4 in SH-SY5Y cells, and the mitochondrial free Ca2+ shifted their levels, tightly coupled with the protein levels of UCPs. When UCP2 and UCP4 were knocked down by siRNA, the result was reversed. These data suggest that the superoxide anion can regulate the mitochondrial free Ca2+ by regulating the expression of UCPs. These observations also indicate that UCPs can be potential targets in pathotherapy prevention of AD. Antioxid. Redox Signal. 11, 1805-1818.
	2009-08-01
语种	英语
发表期刊	ANTIOXIDANTS & REDOX SIGNALING
ISSN	1523-0864
卷号	11 期号:8 页码:1805-1818
期刊论文类型	Article
收录类别	SCI
WOS记录号	WOS:000267488900004
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/13632
专题	中国科学院心理研究所回溯数据库(1956-2010)
作者单位	1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100101, Peoples R China 3.Rutgers State Univ, Dept Cell Biol & Neurosci, Piscataway, NJ USA 4.Chinese Acad Sci, Key Lab Mental Hlth, Beijing 100101, Peoples R China 5.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Univ, Div Nitr Oxide & Inflammatory Med, Shanghai, Peoples R China
第一作者单位	脑与认知科学国家重点实验室
推荐引用方式 GB/T 7714	Wu, Zhaofei,Zhang, Jie,Zhao, Baolu. Superoxide Anion Regulates the Mitochondrial Free Ca2+ Through Uncoupling Proteins[J]. ANTIOXIDANTS & REDOX SIGNALING,2009,11(8):1805-1818.
APA	Wu, Zhaofei,Zhang, Jie,&Zhao, Baolu.(2009).Superoxide Anion Regulates the Mitochondrial Free Ca2+ Through Uncoupling Proteins.ANTIOXIDANTS & REDOX SIGNALING,11(8),1805-1818.
MLA	Wu, Zhaofei,et al."Superoxide Anion Regulates the Mitochondrial Free Ca2+ Through Uncoupling Proteins".ANTIOXIDANTS & REDOX SIGNALING 11.8(2009):1805-1818.