Fasting induces a high level of 3-nitrotyrosine in the brain of rats

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	Fasting induces a high level of 3-nitrotyrosine in the brain of rats
	Liu, Yanying 1,2; He, Rongqiao 1,2
摘要	Although the relationship between hyperglycemia (using diabetic animal model) and plasma nitrotyrosine level has been studied, the effect of hypoglycemia on nitrotyrosine level in the brain has not been addressed. Here, we evaluated nitration of protein, the colocalization of nitration with alpha-synuclein, activity of inducible nitric oxide synthase, and nitric oxide content using fasting and diabetic animal models. The results showed that signals of alpha-synuclein were widely distributed in most parts of the pallium, midbrain, hippocampus and cerebellum, as indicated by immunohistochemistry. Most signals of the 3-nitrotyrosine were colocalized with those of alpha-synuclein in the midbrain of fasting rats. The level of proteins containing 3-nitrotyrosine was significantly increased in the brain of fasting rats in Western blotting, especially in the midbrain, compared with control rats. In addition, the 3-nitrotyrosine signals increased in hippocampus of diabetic rats. Immunoprecipitation showed that alpha-synuclein was nitrated in the fasting rats. The iNOS activity and nitric oxide levels were significantly increased in both fasting and diabetic animals. The enhanced 3-nitrotyrosine level in the brain of fasting rats suggests that nitration of protein including alpha-synuclein in the midbrain is more affected by hypoglycemia in fasting than hyperglycemia in diabetic rats. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
关键词	Nitration 3-Nitrotyrosine alpha-Synuclein Nitric oxide
	2010-03-26
语种	英语
发表期刊	NEUROSCIENCE LETTERS
ISSN	0304-3940
卷号	472 期号:3 页码:204-209
期刊论文类型	Article
收录类别	SCI
WOS记录号	WOS:000276598000012
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/13671
专题	中国科学院心理研究所回溯数据库(1956-2010)
作者单位	1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Psychol, Lab Mental Hlth, Beijing 100101, Peoples R China
第一作者单位	脑与认知科学国家重点实验室; 中国科学院心理健康重点实验室
推荐引用方式 GB/T 7714	Liu, Yanying,He, Rongqiao. Fasting induces a high level of 3-nitrotyrosine in the brain of rats[J]. NEUROSCIENCE LETTERS,2010,472(3):204-209.
APA	Liu, Yanying,&He, Rongqiao.(2010).Fasting induces a high level of 3-nitrotyrosine in the brain of rats.NEUROSCIENCE LETTERS,472(3),204-209.
MLA	Liu, Yanying,et al."Fasting induces a high level of 3-nitrotyrosine in the brain of rats".NEUROSCIENCE LETTERS 472.3(2010):204-209.