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Characterization of the Small RNA Transcriptomes of Androgen Dependent and Independent Prostate Cancer Cell Line by Deep Sequencing
Xu, Gang1; Wu, Jinyu2; Zhou, LingLin2; Chen, Binghua1; Sun, Zhongsheng2,3; Zhao, Fangqing2,4; Tao, Zhihua1
2010-11-30
Source PublicationPLOS ONE
ISSN1932-6203
SubtypeArticle
Volume5Issue:11
AbstractGiven the important roles of miRNA in post-transcriptional regulation and its implications for cancer, characterization of miRNA facilitates us to uncover molecular mechanisms underlying the progression of androgen-independent prostate cancer (PCa). The emergence of next-generation sequencing technologies has dramatically changed the speed of all aspects of sequencing in a rapid and cost-effective fashion, which can permit an unbiased, quantitive and in-depth investigation of small RNA transcriptome. In this study, we used high-throughput Illumina sequencing to comprehensively represent the full complement of individual small RNA and to characterize miRNA expression profiles in both the androgen dependent and independent Pca cell line. At least 83 miRNAs are significantly differentially expressed, of which 41 are up-regulated and 42 are down-regulated, indicating these miRNAs may be involved in the transition of LNCaP to an androgen-independent phenotype. In addition, we have identified 43 novel miRNAs from the androgen dependent and independent PCa library and 3 of them are specific to the androgen-independent PCa. Function annotation of target genes indicated that most of these differentially expressed miRNAs tend to target genes involved in signal transduction and cell communication, epically the MAPK signaling pathway. The small RNA transcriptomes obtained in this study provide considerable insights into a better understanding of the expression and function of small RNAs in the development of androgen-independent prostate cancer.
Indexed BySCI
Language英语
WOS IDWOS:000284755100090
Citation statistics
Cited Times:50[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/13705
Collection中国科学院心理研究所回溯数据库(1956-2010)
Affiliation1.Wenzhou Med Coll, Ctr Clin Lab Diag, Affiliated Hosp 1, Wenzhou, Peoples R China
2.Wenzhou Med Coll, Inst Genom Med, Wenzhou, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Behav Genet Ctr, Beijing 100101, Peoples R China
4.Chinese Acad Sci, Beijing Inst Life Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Xu, Gang,Wu, Jinyu,Zhou, LingLin,et al. Characterization of the Small RNA Transcriptomes of Androgen Dependent and Independent Prostate Cancer Cell Line by Deep Sequencing[J]. PLOS ONE,2010,5(11).
APA Xu, Gang.,Wu, Jinyu.,Zhou, LingLin.,Chen, Binghua.,Sun, Zhongsheng.,...&Tao, Zhihua.(2010).Characterization of the Small RNA Transcriptomes of Androgen Dependent and Independent Prostate Cancer Cell Line by Deep Sequencing.PLOS ONE,5(11).
MLA Xu, Gang,et al."Characterization of the Small RNA Transcriptomes of Androgen Dependent and Independent Prostate Cancer Cell Line by Deep Sequencing".PLOS ONE 5.11(2010).
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