Substrate-induced Changes in Protease Active Site Conformation Impact on Subsequent Reactions with Substrates
Pan, Rong1; Zhang, Xue-Jing1,2; Zhang, Zi-Jian1,3; Zhou, Yuan1,3; Tian, Wei-Xi3; He, Rong-Qiao1,3
2010-07-23
发表期刊JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN0021-9258
文章类型Article
卷号285期号:30页码:22948-22954
摘要Enzymatic catalysis of biochemical reactions is essential to all living systems. The "lock and key" and "induced fit" models were early contributions to our understanding of the mechanisms involved in the reaction between an enzyme and its substrate. However, whether a given substrate-induced conformation is rigid or remains flexible has not yet been determined. By measuring the enzyme activity and intrinsic fluorescence of a nonspecific Eisenia fetida protease-I with different chromogenic substrates, we show that in subsequent reactions of protease with substrates, both the "lock and key" and "induced fit" mechanisms are used depending on the degree of conformational change required. Chromozym-Th-or chromosym-Ch-induced protease conformations were unable to bind chromozym-U. The chromosym-U-induced protease conformation remained flexible and could be further induced by chromozym-Th and chromozym-Ch. When low concentrations of guanidine HCl were used to disturb the conformation of the enzyme, only small changes in intrinsic fluorescence of the chromozym-Th-induced protease were detected, in contrast to the native enzyme whose intrinsic fluorescence markedly increased. This indicates that the substrate-induced enzyme was relatively rigid compared with the native protease. Utilizing a lock and key mechanism for secondary substrate reactions may have adaptive value in that it facilitates high efficiency in enzymatic reactions.
收录类别SCI
语种英语
WOS记录号WOS:000279999900029
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/13719
专题中国科学院心理研究所回溯数据库(1956-2010)
作者单位1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Pan, Rong,Zhang, Xue-Jing,Zhang, Zi-Jian,et al. Substrate-induced Changes in Protease Active Site Conformation Impact on Subsequent Reactions with Substrates[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2010,285(30):22948-22954.
APA Pan, Rong,Zhang, Xue-Jing,Zhang, Zi-Jian,Zhou, Yuan,Tian, Wei-Xi,&He, Rong-Qiao.(2010).Substrate-induced Changes in Protease Active Site Conformation Impact on Subsequent Reactions with Substrates.JOURNAL OF BIOLOGICAL CHEMISTRY,285(30),22948-22954.
MLA Pan, Rong,et al."Substrate-induced Changes in Protease Active Site Conformation Impact on Subsequent Reactions with Substrates".JOURNAL OF BIOLOGICAL CHEMISTRY 285.30(2010):22948-22954.
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