PSYCH OpenIR  > 中国科学院心理健康重点实验室
Proteomic and transcriptomic analysis of visual long-term memory in Drosophila melanogaster
Jiang, Huoqing1,2; Hou, Qinlong1,2; Gong, Zhefeng1; Liu, Li1,3
2011-03-01
Source PublicationPROTEIN & CELL
ISSN1674-800X
SubtypeArticle
Volume2Issue:3Pages:215-222
AbstractThe fruit fly, Drosophila melanogaster, is able to discriminate visual landmarks and form visual long-term memory in a flight simulator. Studies focused on the molecular mechanism of long-term memory have shown that memory formation requires mRNA transcription and protein synthesis. However, little is known about the molecular mechanisms underlying the visual learning paradigm. The present study demonstrated that both spaced training procedure (STP) and consecutive training procedure (CTP) would induce long-term memory at 12 hour after training, and STP caused significantly higher 12-h memory scores compared with CTP. Label-free quantification of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and microarray were utilized to analyze proteomic and transcriptomic differences between the STP and CTP groups. Proteomic analysis revealed 30 up-regulated and 27 down-regulated proteins; Transcriptomic analysis revealed 145 up-regulated and 129 down- regulated genes. Among them, five candidate genes were verified by quantitative PCR, which revealed results similar to microarray. These results provide insight into the molecular components influencing visual long-termmemory and facilitate further studies on the roles of identified genes in memory formation.
KeywordVisual Learning And Memory Drosophila Long-term Memory Microarray Liquid Chromatography-tandem Mass Spectrometry
Indexed BySCI
Language英语
WOS IDWOS:000310519700006
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/13791
Collection中国科学院心理健康重点实验室
Affiliation1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Key Lab Mental Hlth, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Huoqing,Hou, Qinlong,Gong, Zhefeng,et al. Proteomic and transcriptomic analysis of visual long-term memory in Drosophila melanogaster[J]. PROTEIN & CELL,2011,2(3):215-222.
APA Jiang, Huoqing,Hou, Qinlong,Gong, Zhefeng,&Liu, Li.(2011).Proteomic and transcriptomic analysis of visual long-term memory in Drosophila melanogaster.PROTEIN & CELL,2(3),215-222.
MLA Jiang, Huoqing,et al."Proteomic and transcriptomic analysis of visual long-term memory in Drosophila melanogaster".PROTEIN & CELL 2.3(2011):215-222.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jiang, Huoqing]'s Articles
[Hou, Qinlong]'s Articles
[Gong, Zhefeng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jiang, Huoqing]'s Articles
[Hou, Qinlong]'s Articles
[Gong, Zhefeng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jiang, Huoqing]'s Articles
[Hou, Qinlong]'s Articles
[Gong, Zhefeng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.