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Effects of Ginsenoside Rg1 on the Expression of Toll-Like Receptor 3, 4 and Their Signalling Transduction Factors in the NG108-15 Murine Neuroglial Cell Line
Zhao, Bao-Sheng1; Liu, Yang2; Gao, Xiao-Yan1; Zhai, Hua-Qiang2; Guo, Jian-You3; Wang, Xue-Yong2
2014-10-01
发表期刊MOLECULES
ISSN1420-3049
文章类型Article
卷号19期号:10页码:16925-16936
摘要As one of the most important components of Panax ginseng, ginsenoside Rg1 has certain anti-aging effects, improving the activity of learning and memory. Studies have showed that ginsenoside Rg1 improves the memory impairment associated with Alzheimer's disease (AD). In this study, the effects of ginsenoside Rg1 were investigated through the activity of toll-like receptor (TLR) 3, TLR4 and their signaling transduction pathways in amyloid beta peptide 25-35 (A beta(25-35)) induced AD cell model. Thus we investigated several critical components of the TLR pathway. The neuroglial cell line NG108-15 was stimulated with or without A beta(25-35), while different concentrations of ginsenoside Rg1 were administered. After 24 h, tumor necrosis factor-alpha (TNF-alpha), interferon-beta (IFN-beta) in cell supernatant and inducible nitric oxide synthase (iNOS) in cell lysate supernatant were measured with enzyme-linked immunosorbent assays (ELISAs). The mRNA and protein expression of TLR3, TLR4, nuclear factor kappa B (NF-kappa B) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) were detected by real-time PCR and western blot methods, respectively. The experimental results showed that A beta(25-35) could markedly raise the level of TNF-alpha, IFN-alpha and iNOS, and increase the expressions of mRNA and TLR3, TLR4, NF-kappa B and TRAF-6 protein in the NG108-15 cells. At the same time, the ginsenoside Rg1 significantly reduced the expressions of proteins and mRNA of TLR3, TLR4, NF-kappa B and TRAF-6, and down-regulated the levels of TNF-alpha, IFN-alpha of cell supernatant and iNOS of cell lysate supernatant in a concentration-dependent manner. In conclusion, ginsenoside Rg1 has good activity for suppressing the signaling transduction pathway of TLR3 and TLR4, and decreasing the inflammation factors induced by A beta(25-35) in NG108-15 cells, and this may be the mechanism of ginsenoside Rg1 action in AD treatment, but more studies are needed to identify its specificity.
关键词Alzheimer's Disease Ginsenoside Rg1 Toll-like Receptor Nuclear Factor Kappa b Tumor Necrosis Factor Receptor-associated Factor-6
收录类别SCI
语种英语
WOS记录号WOS:000344456100092
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/14251
专题中国科学院心理健康重点实验室
作者单位1.Beijing Univ Chinese Med, Ctr Sci Expt, Beijing 100029, Peoples R China
2.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100102, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
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GB/T 7714
Zhao, Bao-Sheng,Liu, Yang,Gao, Xiao-Yan,et al. Effects of Ginsenoside Rg1 on the Expression of Toll-Like Receptor 3, 4 and Their Signalling Transduction Factors in the NG108-15 Murine Neuroglial Cell Line[J]. MOLECULES,2014,19(10):16925-16936.
APA Zhao, Bao-Sheng,Liu, Yang,Gao, Xiao-Yan,Zhai, Hua-Qiang,Guo, Jian-You,&Wang, Xue-Yong.(2014).Effects of Ginsenoside Rg1 on the Expression of Toll-Like Receptor 3, 4 and Their Signalling Transduction Factors in the NG108-15 Murine Neuroglial Cell Line.MOLECULES,19(10),16925-16936.
MLA Zhao, Bao-Sheng,et al."Effects of Ginsenoside Rg1 on the Expression of Toll-Like Receptor 3, 4 and Their Signalling Transduction Factors in the NG108-15 Murine Neuroglial Cell Line".MOLECULES 19.10(2014):16925-16936.
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