大鼠抑郁障碍与心血管组织炎性标记物的相关性研究 | |
其他题名 | Relationship between depressive disorder and inflammation markers in depression rat model |
陈莹1; 常志文1; 翟艳苓1; 张勇1; 杨琳1; 林文娟1 | |
心理所单位排序 | 5 |
摘要 | 目的探讨强迫游泳抑郁模型大鼠抑郁障碍与炎性标记物的相关性。方法健康SD大鼠随机抽签分为对照组和模型组,应用21 d强迫游泳试验建立大鼠抑郁模型,模型组大鼠包括SS组(9只,应激+生理盐水腹腔注射)、SF组(8只,应激+氟西汀腹腔注射)和SI组(8只,应激+丙咪嗪腹腔注射)。对照组大鼠包括CS组(10只,生理盐水腹腔注射),CI组(7只,丙咪嗪腹腔注射)。应用透射免疫比浊法和酶联免疫法检测外周血炎性标记物高敏C反应蛋白(hsCRP)、单核细胞趋化因子1(MCP-1)水平,应用免疫组织化学染色法和蛋白印迹法检测血管组织核因子-κB(NF-κB)p65、抑制蛋白κB(IκB)-α、MCP-1表达水平。结果应激后模型组外周血hsCRP和MCP-1含量明显高于CS、CI组,SS组与CS组hsCRP水平分别为(0.26±0.07)mg/L和(0.15±0.06)mg/L(P<0.01),胞浆中IκB-α表达显著减少,SS组与CS组IκB-α水平分别为(0.48±0.07)和(2.07±0.48)(P<0.01)。结论抑郁障碍通过NF-κB表达增强来启动炎症反应。 |
其他摘要 | Objective To investigate the relationship between depressive disorder and inflammation markers in depression rat model. Methods Depression rat model was made by Forced Swimming Test (FST) in 21days. A total of 50 Sprague-Dawley rats were randomly divided into 5 groups: CS group (n = 10, control group, treated with saline), CI group (n = 10, control group, treated with imipramine) , SS group (n = 10, model group, treated with saline), SF group (n = 10, model group, treated with fluoxetine) and SI group (n = 10, model group, treated with imipramine). Monocyte chemoattractant protein-1 (MCP- 1 ) in peripheral blood was measured by enzyme linked immunoassay (ELISA) and hypertensive C-reaction protein (hsCRP) by immunoturbidimetric assay. The expression of MCP-1, nuclear factor-kappa B (NF- KB) 1365 and inhibitory kappa B (IKB)-(x in aorta tissue were observed by immunohistochemistry and western blot. Results Eight rats died during the study (3 in CI group, 1 in SS group, 2 in SF group and 2 in SI group). Serum concentration of hsCRP and MCP-1 in peripheral blood was significantly higher [ (0. 26 ± 0. 07) mg/L vs. (0. 15± 0. 06) mg/L for hsCRP and (52. 02 ± 18.83 ) ng/L vs. (22. 52± 8.45 ) ng/L for MCP-1, both P 〈 0. 01 ] and expression of MCP-1 and NF-KB p65 in aorta tissue was significantly increased in SS group than in CS group [ (1.78 ± 0. 36) vs. (0. 85 ±0. 15 ) for MCP-1 and (0. 88 ± 0. 16) vs. (0. 12 ± 0. 04) for NF-KB 1365, both P 〈 0. 01 ]. The expression of IKB-α was significantly decreased in aorta tissue in SS group than in CS group [ (0.48±0. 07) vs. (2. 07±0.48), P 〈0. 01 ]. Conclusions Depression disorder activates inflammatory reaction by increasing expression of NF-KB. |
关键词 | 抑郁症 核因子-κBp65 IκB-α 单核细胞趋化蛋白-1 实验动物模型 |
学科领域 | 医学心理学 |
2012 | |
语种 | 中文 |
发表期刊 | 中国心血管杂志 |
ISSN | 1007-5410 |
卷号 | 17期号:1页码:43-47 |
期刊论文类型 | 期刊论文 |
URL | 查看原文 |
文献类型 | 期刊论文 |
条目标识符 | http://ir.psych.ac.cn/handle/311026/14480 |
专题 | 健康与遗传心理学研究室 |
通讯作者 | 陈莹 |
作者单位 | 1.首都医科大学附属北京同仁医院干部医疗科 2.首都医科大学附属北京同仁医院血液科 3.首都医科大学附属北京同仁医院病理科 4.首都医科大学肝细胞实验室 5.中国科学院心理研究所 |
推荐引用方式 GB/T 7714 | 陈莹,常志文,翟艳苓,等. 大鼠抑郁障碍与心血管组织炎性标记物的相关性研究[J]. 中国心血管杂志,2012,17(1):43-47. |
APA | 陈莹,常志文,翟艳苓,张勇,杨琳,&林文娟.(2012).大鼠抑郁障碍与心血管组织炎性标记物的相关性研究.中国心血管杂志,17(1),43-47. |
MLA | 陈莹,et al."大鼠抑郁障碍与心血管组织炎性标记物的相关性研究".中国心血管杂志 17.1(2012):43-47. |
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