Determination of endogenous corticosterone in rodent's blood, brain and hair with LC-APCI-MS/MS

doi:10.1016/j.jchromb.2015.08.035

	Determination of endogenous corticosterone in rodent's blood, brain and hair with LC-APCI-MS/MS
	Yu, Tian 1,2; Xu, Hang 3; Wang, Weiwen3 ; Li, Shifei 1,2; Chen, Zheng 1,2; Deng, Huihua 1,2,3
第一作者	Yu, Tian
通讯作者邮箱	dengrcls@seu.edu.cn (h. deng).
摘要	Endogenous corticosterone in rodent's hair would be a potential biomarker to assess the response of the hypothalamus-pituitary-adrenal axis to chronic stress. However, currently unknown is whether hair corticosterone is associated with endogenous corticosterone in blood and brain. The present study aimed to develop an enhanced assay for determination of endogenous corticosterone in blood, brain and hair, and to examine associations of hair corticosterone with blood and brain corticosterone under basal condition and association with blood corticosterone under chronic stressful condition. Hair at the back and blood samples were collected from non-stressed and stressed rodents, and prefrontal lobe and thalamus from non-stressed rodents. Chronic stress exerted on mice was 30-day repeated social defeat. The analyses were done using high performance liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization in positive mode. Limits of detection and quantification were 0.2 and 0.5 ng/ml for rat's blood, and 0.5 and 1.0 pg/mg for rat's hair and brain, and 1.25 and 2.50 ng/ml (or pg/mg) for mouse's blood (or hair). Recovery ranged from 84.2 to 108.0%. The intra- and inter-day coefficients of variation were less than 10%. Additionally, correlation of hair corticosterone with blood corticosterone was significant in both mice and rats, but correlations with corticosterone in prefrontal lobe and thalamus were not significant in rats. Both hair and blood corticosterone were significantly higher in stressed mice compared with controls. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Hair Corticosterone Blood Corticosterone Brain Corticosterone Repeated Social Defeat Atmospheric Pressure Chemical Ionization
	2015-10-01
语种	英语
DOI	10.1016/j.jchromb.2015.08.035
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
卷号	1002 期号:0 页码:267-276
期刊论文类型	Article
收录类别	SCI
项目简介	We wish to thank Prof. Wei Lu and Miss Yuehua Zhu (Key Laboratory of Development Genes and Human Diseases, Ministry of Education, Southeast University) for their assistance in rats' sampling. This research was supported by Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Science, and in part by the National Basic Research Program of China under Grant 2015CB351704, and by the Fundamental Research Funds for the Southeast University, and by Jiangsu Provincial Innovation Project for Scientific Research of Graduate Students in Universities (KYZZ_0069), China.
WOS记录号	WOS:000362132100034
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/15266
专题	中国科学院心理健康重点实验室
通讯作者	Deng, Huihua
作者单位	1.Southeast Univ, Key Lab Child Dev & Learning Sci, Minist Educ, Nanjing 210096, Jiangsu, Peoples R China 2.Southeast Univ, Inst Child Dev & Educ, Res Ctr Learning Sci, Nanjing 210096, Jiangsu, Peoples R China 3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
通讯作者单位	中国科学院心理健康重点实验室
推荐引用方式 GB/T 7714	Yu, Tian,Xu, Hang,Wang, Weiwen,et al. Determination of endogenous corticosterone in rodent's blood, brain and hair with LC-APCI-MS/MS[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2015,1002(0):267-276.
APA	Yu, Tian,Xu, Hang,Wang, Weiwen,Li, Shifei,Chen, Zheng,&Deng, Huihua.(2015).Determination of endogenous corticosterone in rodent's blood, brain and hair with LC-APCI-MS/MS.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1002(0),267-276.
MLA	Yu, Tian,et al."Determination of endogenous corticosterone in rodent's blood, brain and hair with LC-APCI-MS/MS".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1002.0(2015):267-276.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
WOS_000362132100034.（1634KB）	期刊论文	出版稿	暂不开放	CC BY-NC-SA	请求全文