PSYCH OpenIR  > 中国科学院心理健康重点实验室
Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference
Tian, Weiping1,2; Wang JS(王杰思)1,3; Zhang, Ke1; Teng, Huajing3; Li, Chong1; Szyf, Moshe5; Sun, Zhong Sheng3,4; Zhao, Mei1
2016-02-25
发表期刊SCIENTIFIC REPORTS
ISSN2045-2322
文章类型Article
卷号6期号:22页码:087
摘要Abnormal BDNF signaling contributes to the structural and behavioral plasticity induced by drugs of abuse. However, the mechanisms regulating expression of Bdnf in drug addiction remain elusive. In the present study, using the conditioned place preference (CPP) model, we showed that expression of Bdnf IV is upregulated in the nucleus accumbens (NAc) of conditioned animals while Bdnf I is upregulated in cocaine-treated mice irrespective of conditioning. The methylation level of a putative c-MYB binding site in the promoter region of Bdnf IV was significantly decreased in the NAc under cocaine CPP conditioning but remained unchanged without conditioning, concurrently with increased binding of c-MYB to this site. Exon IV promoter/luciferase reporter assays revealed that transactivation of Bdnf by c-MYB was blocked by methylation of this c-MYB binding site. Administration of methionine, a precursor of SAM, inhibited cocaine CPP, reversed demethylation of c-MYB binding site and induction of Bdnf IV expression by cocaine CPP. Our results imply that Bdnf IV demethylation at c-MYB binding site is involved in cocaine-triggered seeking behavior, whereas Bdnf I responds to the immediate pharmacological effects of cocaine.
DOI10.1038/srep22087
收录类别SCI
语种英语
项目资助者Natural Science Foundation of China(30870821 ; Key Project of the National 12th Five-Year Research Program of China(2012BAI03B02) ; National Natural Science Foundation of China(91132728) ; Tianjin Municipal Education Commission(20130601) ; 81071078)
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000370800800002
WOS标题词Science & Technology
关键词[WOS]MESOLIMBIC DOPAMINE SYSTEM ; NEUROTROPHIC FACTOR ; DNA METHYLATION ; TRANSCRIPTION FACTOR ; DRUG-ADDICTION ; EPIGENETIC REGULATION ; NUCLEUS-ACCUMBENS ; SPLICE VARIANTS ; BRAIN ; EXPRESSION
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/19586
专题中国科学院心理健康重点实验室
作者单位1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, 16 Lincui Rd, Beijing 100101, Peoples R China
2.Tianjin Med Univ, Tianjin Res Ctr Basic Med Sci, Tianjin 300070, Peoples R China
3.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China
4.Univ Town, Wenzhou Med Coll, Inst Genom Med, Wenzhou 325000, Zhejiang, Peoples R China
5.McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada
推荐引用方式
GB/T 7714
Tian, Weiping,Wang JS,Zhang, Ke,et al. Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference[J]. SCIENTIFIC REPORTS,2016,6(22):087.
APA Tian, Weiping.,Wang JS.,Zhang, Ke.,Teng, Huajing.,Li, Chong.,...&Zhao, Mei.(2016).Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference.SCIENTIFIC REPORTS,6(22),087.
MLA Tian, Weiping,et al."Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference".SCIENTIFIC REPORTS 6.22(2016):087.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
srep22087.pdf(1297KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Tian, Weiping]的文章
[Wang JS(王杰思)]的文章
[Zhang, Ke]的文章
百度学术
百度学术中相似的文章
[Tian, Weiping]的文章
[Wang JS(王杰思)]的文章
[Zhang, Ke]的文章
必应学术
必应学术中相似的文章
[Tian, Weiping]的文章
[Wang JS(王杰思)]的文章
[Zhang, Ke]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。