Institutional Repository of Key Laboratory of Mental Health, CAS
Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference | |
Tian, Weiping1,2; Wang JS(王杰思)1,3; Zhang, Ke1; Teng, Huajing3; Li, Chong1; Szyf, Moshe5; Sun, Zhong Sheng3,4; Zhao, Mei1 | |
第一作者 | Tian, Weiping ; Wang, Jiesi |
通讯作者邮箱 | zhaom@psych.ac.cn |
心理所单位排序 | 1 |
摘要 | Abnormal BDNF signaling contributes to the structural and behavioral plasticity induced by drugs of abuse. However, the mechanisms regulating expression of Bdnf in drug addiction remain elusive. In the present study, using the conditioned place preference (CPP) model, we showed that expression of Bdnf IV is upregulated in the nucleus accumbens (NAc) of conditioned animals while Bdnf I is upregulated in cocaine-treated mice irrespective of conditioning. The methylation level of a putative c-MYB binding site in the promoter region of Bdnf IV was significantly decreased in the NAc under cocaine CPP conditioning but remained unchanged without conditioning, concurrently with increased binding of c-MYB to this site. Exon IV promoter/luciferase reporter assays revealed that transactivation of Bdnf by c-MYB was blocked by methylation of this c-MYB binding site. Administration of methionine, a precursor of SAM, inhibited cocaine CPP, reversed demethylation of c-MYB binding site and induction of Bdnf IV expression by cocaine CPP. Our results imply that Bdnf IV demethylation at c-MYB binding site is involved in cocaine-triggered seeking behavior, whereas Bdnf I responds to the immediate pharmacological effects of cocaine. |
2016-02-25 | |
DOI | 10.1038/srep22087 |
发表期刊 | SCIENTIFIC REPORTS |
ISSN | 2045-2322 |
卷号 | 6期号:22页码:087 |
收录类别 | SCI |
WOS关键词 | MESOLIMBIC DOPAMINE SYSTEM ; NEUROTROPHIC FACTOR ; DNA METHYLATION ; TRANSCRIPTION FACTOR ; DRUG-ADDICTION ; EPIGENETIC REGULATION ; NUCLEUS-ACCUMBENS ; SPLICE VARIANTS ; BRAIN ; EXPRESSION |
WOS标题词 | Science & Technology |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000370800800002 |
Q分类 | Q1 |
资助机构 | Natural Science Foundation of China(30870821 ; Key Project of the National 12th Five-Year Research Program of China(2012BAI03B02) ; National Natural Science Foundation of China(91132728) ; Tianjin Municipal Education Commission(20130601) ; 81071078) |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.psych.ac.cn/handle/311026/19586 |
专题 | 中国科学院心理健康重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, 16 Lincui Rd, Beijing 100101, Peoples R China 2.Tianjin Med Univ, Tianjin Res Ctr Basic Med Sci, Tianjin 300070, Peoples R China 3.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China 4.Univ Town, Wenzhou Med Coll, Inst Genom Med, Wenzhou 325000, Zhejiang, Peoples R China 5.McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada |
第一作者单位 | 中国科学院心理健康重点实验室 |
推荐引用方式 GB/T 7714 | Tian, Weiping,Wang JS,Zhang, Ke,et al. Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference[J]. SCIENTIFIC REPORTS,2016,6(22):087. |
APA | Tian, Weiping.,Wang JS.,Zhang, Ke.,Teng, Huajing.,Li, Chong.,...&Zhao, Mei.(2016).Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference.SCIENTIFIC REPORTS,6(22),087. |
MLA | Tian, Weiping,et al."Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference".SCIENTIFIC REPORTS 6.22(2016):087. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
srep22087.pdf(1297KB) | 期刊论文 | 作者接受稿 | 限制开放 | CC BY-NC-SA | 请求全文 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论