| 其他摘要 | Abstract: There are a lot of negative effect from drug abuse preganant women, causing many neural developmental changes such as altered addictive susceptibility and learinging memory cognitive disfunction. Different action mechnism exsited between different drugs, morphine need to binding μ receptor expression on GABA interneuron, disinhibit DA neuron increase DA release in the synaptic cleft; Cocaine is the inhibitor of DAT, increase DA level through block the transport of DA. Although different action, it finaly have reward effect come out. There is a clear time clue of neurotransmitter development in chick brain, and advantage of chick model in studying addictive mechanism and learning memory. Exposure to morphine or cocaine in different developmental periods of chick embryo,we use conditioned place preference(CPP) to test addictive susceptibility, localmoter activity(LA) and Pecking model(Pecking) to test pharmacological sensitivity to drugs, one trial passive avoidance leaning(PAL) to test the short-term, mid-term and long-term memory. Then use immnohistochemistry to test GABA positive neuron amount. It was found that: 1, E5-9, E10-14, E15-19 morphine exposure increase morphine induced the aciquisition and maintance of CPP; E10-14 cocaine exposure damage the aciquistion of cocaine induced CPP, E5-9, E10-14 and E15-19 cocaine exposure damage the maintainance of cocaine induced CPP; prenatal morphine exposure not effect the acquisition and maintainance of cocaine induced CPP, and prenatal cocaine exposure increase the acquisition and maintainance of morphine induced CPP. 2, Prenatal morphine exposure(E5-9, E10-14, E15-19) increase the pharmacological sensitivity to morphine in Pecking activity, LA increased in E15-19 morphine exposure, prenatal cocaine exposure exposure have no effect in cocaine induced pharmacological sensitivity, prenatal morphine exposure have no effect on cocaine induced pharmacological sensitivity, E15-19 cocaine exposure damage morphine induced LA. 3, E5-9 and E10-14 morphine exposure damage short-term memory, no effect on mid-term and long-term memory, E5-9 cocaine exposure damage short-term memory, have no effect on mid-term memory, and all three periond cocaine exposure damage long-term memories. 4, E15-19 cocaine exposure decrease the expression of GABA-positive neuron in IMHV and Mst, but E15-19 morphine exposure have no effect. |
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