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先前经验对大鼠痛行为的调节及神经机制初探
其他题名Effect of prior experience on pain perception in rats
李胜光
学位类型博士
导师罗非
2011-05
学位授予单位中国科学院研究生院
学位授予地点北京
学位专业心理学
关键词慢性痛 自发痛 条件化 多通道神经元同步记录
摘要先前的疼痛经验可能是影响将来疼痛相关心理疾患重要的因素。过去的动物研究表明幼年期疼痛体验对成年后伤害性反应的影响,但是在成年之后获得的疼痛经验与动物将来的痛觉感知之间的关系仍不清楚。我们研究先前慢性炎症痛经验对以后的福尔马林自发痛和疼痛暗示诱发的预期性反应的影响。并考察了与疼痛无关的慢性应激对大鼠痛行为的影响。最后通过多通道记录技术,在健康大鼠上考察再次伤害性刺激对疼痛暗示诱发中枢神经元放电活动的改变,以初步了解疼痛经验对中枢痛觉信息编码的调节机制。 主要结果: 1. 成年大鼠在慢性炎症痛恢复后,福尔马林痛的第二时相反应显著增强,但第一时相反应无显著改变。 2. 成年大鼠在慢性疼痛史可易化tone-laser条件化的建立,增强条件化反应,并促进疼痛相关的条件化行为的持久维持。慢性炎症痛后期的痛觉过敏与之后持久维持的条件反应水平显著相关。 3. 慢性疼痛早期的连续吗啡给予可有效阻断之后福尔马林痛和疼痛相关的条件反应的增强。 4. 先前的慢性温和应激抑制疼痛相关的条件反应,后者与先前应激的程度显著正相关。 5. 对疼痛的预期可显著增强双侧BLA、对侧mPFC、SI和VPL神经元对伤害性CO2激光脉冲刺激的反应。 6. 再次的反复伤害性激光刺激不仅可使已消退的条件反应恢复,而且可显著增加疼痛暗示所诱发的BLA、mPFC和SI反应神经元的比例。cue诱发的BLA反应维持刺激前水平,SI神经元反应强度的显著增加。BLA-mPFC、SI-BLA之间呈显著交互相关的神经元比例也显著增加。 根据上述结果我们可以得到如下的结论: 1. 首次发现成年大鼠慢性疼痛史能加重以后的自发痛,并持久地增强疼痛相关的预期性和情绪性反应。 2. 首次发现慢性痛后期的痛敏程度预示了将来疼痛疾患的易感性,而慢性痛早期的镇痛处理可有效阻断慢性疼痛史的持久负性影响。 3. 与疼痛无关的慢性温和应激减弱大鼠的疼痛情绪-动机。 4. 应用神经元同步记录技术,观察再次伤害性刺激对大鼠痛情绪中枢反应模式的改变,首次发现除了杏仁基底外侧核,再次的疼痛体验可重塑条件刺激引起的躯体感觉皮层神经元反应模式。 
其他摘要The pain history was an important predictive factor of future pain-related distress. Previous animal studies have illustrated the modulatory effect of neonatal pain experience on subsequent pain-related behaviors. However, the relationship between chronic pain status in adulthood and future pain perception remains unclear. In the current study, we investigated the effects of previous chronic inflammatory pain on the subsequent formalin-evoked pain behaviors and cue-elicited anticipatory responses in adult rats. In addition, a multi-channel single-unit recording technique was used to investigate the possible mechanism underlying the effect of pain experience on pain-related expectation in healthy rats. Four important findings emerged from this study: 1. We demonstrated an increase of the second but not the first phase of the formalin-induced pain behaviors in animals with a history of recovered chronic inflammatory pain. 2. Rats with a chronic pain experience also displayed a facilitated acquisition, an elevated anticipatory response, and a persistent retention of pain-related conditioning behaviors. The hyperalgesia in the late phase of the previous inflammatory pain that correlated with the level of sustained high vigilance. 3. The long-term detrimental influences of prior pain experience could be successfully prevented by successive morphine administration at the early stage of chronic pain. 4. Prior chronic mild stress inhibited subsequent the acquisition and retention of pain-related conditioned response, which was significant correlated with the degree of stress. 5. Under anticipation condition, the neuronal activities to actual noxious stimuli were enhanced in all the recorded areas, including mPFC, BLA, SI and VPL. 6. The repetition of noxious laser stimulation significantly not only resulted in the reinstatement of conditioned response but also the increase in the neuronal activities to auditory cue in BLA and SI areas. The cross-correlation analysis also demonstrated the increase in correlated neuronal activities between BLA, mPFC, and SI after repetition of noxious laser stimulation. In conclusion, it is the first report that chronic pain history exacerbated the subsequent spontaneous pain in adult rats, and exerted long-term effects on the anticipatory and affective response related to pain. All the long-term detrimental effects of chronic pain could be effectively prevented by early-stage pain relief. We first confirmed that the extent of hyperalgesia in the late stage of previous chronic pain was a good tool for prognosis of future susceptibility to pain disorders. In addition to basolateral amygdala, for the first time, we found that the CS-induced neuronal activities in primary somatosensory cortex could be reshaped by pain experience.  
学科领域认知神经科学
语种中文
文献类型学位论文
条目标识符http://ir.psych.ac.cn/handle/311026/20291
专题健康与遗传心理学研究室
作者单位中国科学院心理研究所
推荐引用方式
GB/T 7714
李胜光. 先前经验对大鼠痛行为的调节及神经机制初探[D]. 北京. 中国科学院研究生院,2011.
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