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Simultaneous HPLC-APCI-MS/MS quantification of endogenous cannabinoids and glucocorticoids in hair
Mwanza, Christopher1,2,3; Chen, Zheng1,2; Zhang, Quan1,2; Chen, Shenghuo1,2; Wang, Weiwen4; Deng, Huihua1,2,4; H. Deng
2016-08-15
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Correspondent Emaildengrcls@seu.edu.cn
ISSN1570-0232
SubtypeArticle
Volume1028Issue:0Pages:1-10
AbstractHair matrix could retrospectively record association of endogenous cannabinoids (e.g. 2-arachidonoyl glycerol, 2-AG and N-arachidonoyl-ethanolamine, AEA) and glucocorticoids (e.g. cortisol and cortisone) in a myriad of physiological functions. However, depending on the extraction conditions, the spontaneous isomerization of 2-AG to 1-arachidonoylglycerol (1-AG) and the possible rearrangement of O-arachidonoyl ethanolamine (OAEA) to AEA in various sample matrices could be major obstacles encountered in the detection of both 2-AG and AEA. This study aimed to develop a novel method for simultaneous quantification of 2-AG, AEA, cortisol and cortisone in hair. Methanol was used as the incubation solution and an acidic mixture of deionized water and methanol were utilized as mobile phase in order to avert possible rearrangements of both OAEA and 2-AG. The analyses were performed on a high-performance liquid chromatography tandem mass spectrometer with atmosphere pressure chemical ionization in positive mode. The method showed good linearity in the range of 3.0-250 pg/mg for AEA, 15.0-1250 pg/mg for 2-AG and 1-250 pg/mg for cortisol and cortisone. Limit of detection was 1.5 pg/mg for AEA, 6.0 pg/mg for 2-AG and 0.5 pg/mg for cortisol and cortisone. For all four analytes, intra and inter-day coefficients of variation were less than 20% and recovery above 90%. Population analyses in 473 hair samples established that 2-AG was significantly correlated with AEA. 2-AG was significantly and positively correlated with cortisol and cortisone. There was a significant positive correlation of AEA with cortisol, but not with cortisone. Obese participants showed a significantly higher concentration of cortisone and 2-AG. Males showed significantly higher 2-AG and cortisone levels but significantly lower AEA levels than females. (C) 2016 Elsevier B.V. All rights reserved.
Keyword2-Arachidonoyl glycerol N-arachidonoyl-ethanolamine Cortisone Cortisol Hair matrix
DOI10.1016/j.jchromb.2016.06.002
Indexed BySCI
Language英语
Funding OrganizationNational Basic Research Program of China(2015CB351704) ; Humanities and Social Science Foundation(15YJAZH009) ; Ministry of Education ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Science ; Jiangsu Provincial Innovation Project for Scientific Research of Graduate Students in Universities(KYZZ14_0069 ; Fundamental Research Funds for Southeast University, Ministry of Education, China ; Chinese government ; University of Zambia ; KYZZ15_0054)
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000380593600001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; ENDOCANNABINOID SYSTEM ; 11-BETA-HYDROXYSTEROID DEHYDROGENASE ; CHRONIC STRESS ; HUMAN PLASMA ; QUANTITATIVE METHOD ; ENZYMATIC-SYNTHESIS ; RECEPTOR AGONIST ; FAT DISTRIBUTION
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Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/20334
Collection中国科学院心理健康重点实验室
Corresponding AuthorH. Deng
Affiliation1.Southeast Univ, Minist Educ, Key Lab Child Dev & Learning Sci, Nanjing 210096, Jiangsu, Peoples R China
2.Southeast Univ, Res Ctr Learning Sci, Inst Child Dev & Educ, Nanjing 210096, Jiangsu, Peoples R China
3.Univ Zambia, Sch Nat Sci, Dept Chem, Lusaka 10100, Zambia
4.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Mwanza, Christopher,Chen, Zheng,Zhang, Quan,et al. Simultaneous HPLC-APCI-MS/MS quantification of endogenous cannabinoids and glucocorticoids in hair[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1028(0):1-10.
APA Mwanza, Christopher.,Chen, Zheng.,Zhang, Quan.,Chen, Shenghuo.,Wang, Weiwen.,...&H. Deng.(2016).Simultaneous HPLC-APCI-MS/MS quantification of endogenous cannabinoids and glucocorticoids in hair.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1028(0),1-10.
MLA Mwanza, Christopher,et al."Simultaneous HPLC-APCI-MS/MS quantification of endogenous cannabinoids and glucocorticoids in hair".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1028.0(2016):1-10.
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