|Alternative Title||Study on syndrome of stagnation of liver qi and spleen deficiency in depression based on corticosterone-inflammation-mitochondrial network and effects of Xingpi Jieyu Decoction|
|袁清洁1; 郭建友2; 王建伟1; 李阳1; 邓潇斐2; 郭蓉娟3|
Abstract: Objective: To study the pathogenesis of stagnation of liver qi and spleen deficiency and the effect of Xingpi Jieyu Decoction on depression based on the changes in corticosterone-inflammation-mitochondrial network. Methods: A 6-week chronic unpredictable mild stress (CUMS) method was used to establish a rat model of depression which met the syndrome of stagnation of liver qi and spleen deficiency. The serum was collected at end of the 2nd, 4th and 6th week, and the levels of CORT, TNF-α, IFN-γ, IL-4, IL-6 and IL-10 in serum were detected by Elisa. At 2 and 6 weeks, the prefrontal cortex and the liver tissue were harvested for the observation of the mitochondrial ultrastructure under transmission electron microscopy. Results: The levels of CORT, TNF-α, IFN-γ and IL-6 in serum were significantly increased (P<0.01) and IL-10 levels decreased (P<0.05) when rats were suffered from 2-week CUMS. Meanwhile, the mitochondria in the neuron of prefrontal cortex and the liver cell were slightly damaged. The levels of CORT, TNF-α, IFN-γ and IL-6 in serum were significantly increased (P<0.01) and the levels of IL-4 and IL-10 were significantly decreased (P<0.01) when rats were suffered from 6-week CUMS. Meanwhile, the mitochondria in the neuron of the prefrontal cortex and the liver cell were obviously damaged. Xingpi Jieyu Decoction early antagonized the increase of corticosterone and inhibited inflammation and mitochondrial damage. Conclusion: The CORT content is increased obviously and the inflammatory network is partially disturbed at the early stage of stress. The corticosterone-inflammation-mitochondrial network was imbalanced obviously at the late stage of stress, which manifested the syndrome of stagnation of liver qi and spleen deficiency. The Chinese medicine compound formula Xingpi Jieyu Decoction played obvious protective effect on the stressed rats.
|Keyword||皮质酮 炎性反应 线粒体 抑郁症 肝郁脾虚 肝气郁结 肝脾相关|
|袁清洁,郭建友,王建伟,等. 基于皮质酮-炎性反应-线粒体网络研究抑郁症肝郁脾虚病机及醒脾解郁方干预效应[J]. 中华中医药杂志,2017,32(5):2241-2245.|
|MLA||袁清洁,et al."基于皮质酮-炎性反应-线粒体网络研究抑郁症肝郁脾虚病机及醒脾解郁方干预效应".中华中医药杂志 32.5(2017):2241-2245.|
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