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Adolescent social isolation affects schizophrenia-like behavior and astrocyte biomarkers in the PFC of adult rats
Sun, Lan1; Min, Li2; Zhou, Hao1; Li, Man3; Shao, Feng1; Wang, Weiwen4; F. Shao; W. Wang
2017-08-30
Source PublicationBEHAVIOURAL BRAIN RESEARCH
Correspondent Emailshaof@pku.edu.cn ; wangww@psych.ac.cn
ISSN0166-4328
SubtypeArticle
Volume333Issue:0Pages:258-266
Abstract

Social isolation is regarded as a cause of schizophrenia spectrum disorders. Animal models of schizophrenia are constructed by repeated early environment deprivation as an important paradigm to reveal its pathological mechanism. Male Sprague Dawley rats were assigned to either social-rearing (SR) or isolated-rearing (IR) groups during postnatal days (PNDs) 21-34. On PND 56, all rats underwent behavioral testing including locomotor activity, anxiety-related behaviors in an open field and prepulse inhibition (PPI). Then, the rats were sacrificed and prefrontal cortex (PFC) tissues were separated for high-throughput proteomics analysis and Western blot validation. Rats of the IR group showed increased spontaneous locomotion, increased anxiety-like behavior and disrupted PPI compared with rats of the SR group. Based on proteomics analysis, a total of 124 PFC proteins were found to be significantly differentially expressed between the SR group and the IR group, the most remarkable of which were glial fibrillary acidic protein (GFAP), Annexin A2 (ANXA2) and vimentin (VIM), three astrocyte biomarkers. Further Western blot measurement confirmed that the levels of GFAP, ANXA2 and VIM were increased significantly in IR rats. Adolescent social isolation induced schizophrenia-like behaviors and significantly different expression of 124 PFC proteins in adult rats, especially GFAP, ANXA2 and VIM, which suggests that astrocyte development might be involved in the neural mechanism of schizophrenia.

KeywordSchizophrenia Adolescent social isolation PPI Prefrontal cortex Proteomic analysis Astrocyte development
DOI10.1016/j.bbr.2017.07.011
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(31470988 ; Beijing Key Laboratory of Behavior and Mental Health, the Chinese Academy of Sciences(KJZD-EW-L04) ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences ; 81471122)
WOS Research AreaBehavioral Sciences ; Neurosciences & Neurology
WOS SubjectBehavioral Sciences ; Neurosciences
WOS IDWOS:000408289600032
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordDEVELOPMENTAL NEUROPSYCHIATRIC DISORDERS ; FIBRILLARY ACIDIC PROTEIN ; MEDIAL PREFRONTAL CORTEX ; PREPULSE INHIBITION ; NUCLEUS-ACCUMBENS ; LATENT INHIBITION ; EXPRESSION ; DISEASE ; STRESS ; MICE
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/21898
Collection中国科学院心理健康重点实验室
Corresponding AuthorF. Shao; W. Wang
Affiliation1.Peking Univ, Beijing Key Lab Behav & Mental Hlth, Sch Psychol & Cognit Sci, 5 Yiheyuan Rd, Beijing 100871, Peoples R China
2.Capital Med Univ, Beijing Friendship Hosp, Dept Gastroenterol, Beijing 100050, Peoples R China
3.Tianjin Normal Univ, Coll Educ Sci, Dept Psychol, Tianjin 300387, Peoples R China
4.Univ Chinese Acad Sci, Inst Psychol, CAS Key Lab Mental Hlth, 16 Lincui Rd, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Sun, Lan,Min, Li,Zhou, Hao,et al. Adolescent social isolation affects schizophrenia-like behavior and astrocyte biomarkers in the PFC of adult rats[J]. BEHAVIOURAL BRAIN RESEARCH,2017,333(0):258-266.
APA Sun, Lan.,Min, Li.,Zhou, Hao.,Li, Man.,Shao, Feng.,...&W. Wang.(2017).Adolescent social isolation affects schizophrenia-like behavior and astrocyte biomarkers in the PFC of adult rats.BEHAVIOURAL BRAIN RESEARCH,333(0),258-266.
MLA Sun, Lan,et al."Adolescent social isolation affects schizophrenia-like behavior and astrocyte biomarkers in the PFC of adult rats".BEHAVIOURAL BRAIN RESEARCH 333.0(2017):258-266.
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