中国科学院心理健康重点实验室知识库

目的比较丰富环境对野生小家鼠和驯化小鼠空间学习记忆能力的影响。方法为控制丰富环境,实验选用了野生小家鼠F1代。断奶后,将野生小家鼠F1代和昆明小鼠(由野生小家鼠驯化而来)随机放人普通环境和丰富环境内饲养,1个月后进行水迷宫实验:野生普通组(n=6)、野生丰富组(n=7)、昆明普通组(n=10)、昆明丰富组(n=10)。结果(1)记忆阶段,早期丰富饲养环境可以显著缩短野生小家鼠找到站台的潜伏期[普通环境(39.5±3.8)s;丰富环境(25.2±4.5)s;F(1,22)=8.115,P〈0.01],但是对昆明小鼠没有显著影响[普通环境(8.5±2.4)s;丰富环境(7.5±1.7)s;F(1,36)=0.149,P=0.702];(2)学习阶段,早期丰富饲养环境可以显著缩短野生小家鼠(F(1,32)=15.307,P〈0.01)和昆明小鼠(F(1,216)=8.701,P〈0.01)找到站台的潜伏期。结论早期丰富饲养环境可以提高小鼠的空间学习记忆能力,且对野生小家鼠的影响更大。因此,在丰富环境相关研究中,野生动物模型具有更好的效度。

Objective To evaluate and compare the effect of early environmental enrichment on spatial learning and memory in wild house mouse and Kunming mouse, one kind of laboratory mouse which evolved from wild house mouse. Methods The wild house mouse F1 generation were employed to control the environmental enrichment . Offspring were weaned on PND22 and housed in usual or rich environment for one month randomly, then engaged the morris maze: house mouse rich environment( n = 7 ) , house mouse poor environment ( n = 6 ) , Kunming mouse rich environment( n= 10) ,Kunming mouse poor environment( n= 10). Results (1) Early environmental enrichment improve the performance of house mouse ( poor environment : ( 39.5 ± 3.8 ) s ; rich environment : (25.2 ±4.5 ) s; F(1,22) = 8.115, P 〈 0.01 ), but had no effect on that of Kunming mouse in tests of spatial memory ( poor environment : ( 8.5 ± 2.4) s ; rich environment : (7.5 ± 1.7 ) s ; F(1,36) = 0. 149, P = 0. 702). (2) Early environmental enrichment improve the performance of house mouse ( F(1,132) = 15. 307, P〈 0.01 )and Kunming mouse ( F(1,216) = 8. 701, P 〈 0.01 )in spatial learn session. Conclusion The wild house mouse is more sensitive to the enrich environment than laboratory mouse. Therefore, it has higher validity of model.