PSYCH OpenIR  > 中国科学院心理健康重点实验室
Role of Dorsal Striatum Histone Deacetylase 5 in Incubation of Methamphetamine Craving
Li, Xuan1; Carreria, Maria B.2; Witonsky, Kailyn R.1; Zeric, Tamara1; Lofaro, Olivia M.1; Bossert, Jennifer M.1; Zhang, Jianjun3; Surjono, Felicia1; Richie, Christopher T.1; Harvey, Brandon K.1; Son, Hyeon4; Cowan, Christopher W.5; Nestler, Eric J.6; Shaham, Yavin1
First AuthorLi, Xuan
2018
Source PublicationBiological psychiatry
ISSN0006-3223
SubtypeArticle
Issue17Pages:32314-4
QuartileQ1
Contribution Rank3
Abstract

BACKGROUND: Methamphetamine (meth) seeking progressively increases after withdrawal (incubation of meth craving). We previously demonstrated an association between histone deacetylase 5 (HDAC5) gene expression in the rat dorsal striatum and incubation of meth craving. Here we used viral constructs to study the causal role of dorsal striatum HDAC5 in this incubation.

METHODS: In experiment 1 (overexpression), we injected an adeno-associated virus bilaterally into dorsal striatum to express either green fluorescent protein (control) or a mutant form of HDAC5, which strongly localized to the nucleus. After training rats to self-administer meth (10 days, 9 hours/day), we tested the rats for relapse to meth seeking on withdrawal days 2 and 30. In experiment 2 (knockdown), we injected an adeno-associated virus bilaterally into the dorsal striatum to express a short hairpin RNA either against luciferase (control) or against HDAC5. After training rats to self-administer meth, we tested the rats for relapse on withdrawal days 2 and 30. We also measured gene expression of other HDACs and potential HDAC5 downstream targets.

RESULTS: We found that HDAC5 overexpression in dorsal striatum increased meth seeking on withdrawal day 30 but not day 2. In contrast, HDAC5 knockdown in the dorsal striatum decreased meth seeking on withdrawal day 30 but not on day 2; this manipulation also altered other HDACs (Hdac1 and Hdac4) and potential HDAC5 targets (Gnb4 and Suv39h1).

CONCLUSIONS: Results demonstrate a novel role of dorsal striatum HDAC5 in incubation of meth craving. These findings also set up future work to identify HDAC5 targets that mediate this incubation

KeywordDorsal striatum Epigenetics HDAC5 Incubation Methamphetamine Relapse
DOI10.1016/j.biopsych.2017.12.008
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/25708
Collection中国科学院心理健康重点实验室
Corresponding AuthorLi, Xuan
Affiliation1.National Institute on Drug Abuse, National Institutes of Health
2.University of Texas Southwestern Medical Center
3.Institute of Psychology, Chinese Academy of Sciences
4.College of Medicine, Hanyang University
5.Medical University of South Carolina
6.Icahn School of Medicine at Mount Sinai
Recommended Citation
GB/T 7714
Li, Xuan,Carreria, Maria B.,Witonsky, Kailyn R.,et al. Role of Dorsal Striatum Histone Deacetylase 5 in Incubation of Methamphetamine Craving[J]. Biological psychiatry,2018(17):32314-4.
APA Li, Xuan.,Carreria, Maria B..,Witonsky, Kailyn R..,Zeric, Tamara.,Lofaro, Olivia M..,...&Shaham, Yavin.(2018).Role of Dorsal Striatum Histone Deacetylase 5 in Incubation of Methamphetamine Craving.Biological psychiatry(17),32314-4.
MLA Li, Xuan,et al."Role of Dorsal Striatum Histone Deacetylase 5 in Incubation of Methamphetamine Craving".Biological psychiatry .17(2018):32314-4.
Files in This Item:
File Name/Size DocType Version Access License
2017 BP Role of Dors(730KB)期刊论文作者接受稿限制开放CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Xuan]'s Articles
[Carreria, Maria B.]'s Articles
[Witonsky, Kailyn R.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Xuan]'s Articles
[Carreria, Maria B.]'s Articles
[Witonsky, Kailyn R.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Xuan]'s Articles
[Carreria, Maria B.]'s Articles
[Witonsky, Kailyn R.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 2017 BP Role of Dorsal Striatum Histone Deacetylase 5 in Incubation of Methamphetamine Craving.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.