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S-adenosyl-methionine (SAM) alters the transcriptome and methylome and specifically blocks growth and invasiveness of liver cancer cells
Wang, Yan1,2; Sun, ZhongSheng3; Szyf, Moshe2; Moshe Szyf
2017-12-19
Source PublicationONCOTARGET
Correspondent Emailmoshe.szyf@mcgill.ca
ISSN1949-2553
SubtypeArticle
Volume8Issue:67Pages:111866-111881
Contribution Rank1
Abstract

S-adenosyl methionine (SAM) is a ubiquitous methyl donor that was reported to have chemo-protective activity against liver cancer, however the molecular footprint of SAM is unknown. We show here that SAM selectively inhibits growth, transformation and invasiveness of hepatocellular carcinoma cell lines but not normal primary liver cells. Analysis of the transcriptome of SAM treated and untreated liver cancer cell lines HepG2 and SKhep1 and primary liver cells reveals pathways involved in cancer and metastasis that are upregulated in cancer cells and are downregulated by SAM. Analysis of the methylome using bisulfite mapping of captured promoters and enhancers reveals that SAM hyper-methylates and downregulates genes in pathways of growth and metastasis that are upregulated in liver cancer cells. Depletion of two SAM downregulated genes STMN1 and TAF15 reduces cellular transformation and invasiveness, providing evidence that SAM targets are genes important for cancer growth and invasiveness. Taken together these data provide a molecular rationale for SAM as an anticancer agent.

Keywordepigenetic S-adenosyl methionine liver cancer mRNA sequencing capture bisulfite sequencing
DOI10.18632/oncotarget.22942
Indexed BySCI
Language英语
Funding OrganizationChina-Canada team grant from the Canadian Institute of Health Research ; National Science Fund of China
WOS Research AreaOncology ; Cell Biology
WOS SubjectOncology ; Cell Biology
WOS IDWOS:000419567000090
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordHUMAN HEPATOCELLULAR-CARCINOMA ; CHOLINE-DEVOID DIET ; ACID-DEFINED DIETS ; BREAST-CANCER ; EXPRESSION ANALYSIS ; DNA METHYLATION ; BINDING PROTEIN ; RAS ONCOGENES ; GENOME-WIDE ; IN-VITRO
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Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/26031
Collection中国科学院心理健康重点实验室
Corresponding AuthorMoshe Szyf
Affiliation1.Chinese Acad Sci, Inst Psychol, CAS Key Lab Mental Hlth, Beijing, Peoples R China
2.McGill Univ Montreal, Dept Pharmacol & Therapeut, Montreal, PQ, Canada
3.Chinese Acad Sci, Beijing Inst Life Sci, Beijing, Peoples R China
First Author AffilicationKey Laboratory of Mental Health, CAS
Recommended Citation
GB/T 7714
Wang, Yan,Sun, ZhongSheng,Szyf, Moshe,et al. S-adenosyl-methionine (SAM) alters the transcriptome and methylome and specifically blocks growth and invasiveness of liver cancer cells[J]. ONCOTARGET,2017,8(67):111866-111881.
APA Wang, Yan,Sun, ZhongSheng,Szyf, Moshe,&Moshe Szyf.(2017).S-adenosyl-methionine (SAM) alters the transcriptome and methylome and specifically blocks growth and invasiveness of liver cancer cells.ONCOTARGET,8(67),111866-111881.
MLA Wang, Yan,et al."S-adenosyl-methionine (SAM) alters the transcriptome and methylome and specifically blocks growth and invasiveness of liver cancer cells".ONCOTARGET 8.67(2017):111866-111881.
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