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S-adenosyl-methionine (SAM) alters the transcriptome and methylome and specifically blocks growth and invasiveness of liver cancer cells | |
Wang, Yan1,2; Sun, ZhongSheng3; Szyf, Moshe2; Moshe Szyf | |
第一作者 | Wang, Yan |
通讯作者邮箱 | moshe.szyf@mcgill.ca |
心理所单位排序 | 1 |
摘要 | S-adenosyl methionine (SAM) is a ubiquitous methyl donor that was reported to have chemo-protective activity against liver cancer, however the molecular footprint of SAM is unknown. We show here that SAM selectively inhibits growth, transformation and invasiveness of hepatocellular carcinoma cell lines but not normal primary liver cells. Analysis of the transcriptome of SAM treated and untreated liver cancer cell lines HepG2 and SKhep1 and primary liver cells reveals pathways involved in cancer and metastasis that are upregulated in cancer cells and are downregulated by SAM. Analysis of the methylome using bisulfite mapping of captured promoters and enhancers reveals that SAM hyper-methylates and downregulates genes in pathways of growth and metastasis that are upregulated in liver cancer cells. Depletion of two SAM downregulated genes STMN1 and TAF15 reduces cellular transformation and invasiveness, providing evidence that SAM targets are genes important for cancer growth and invasiveness. Taken together these data provide a molecular rationale for SAM as an anticancer agent. |
关键词 | epigenetic S-adenosyl methionine liver cancer mRNA sequencing capture bisulfite sequencing |
2017-12-19 | |
DOI | 10.18632/oncotarget.22942 |
发表期刊 | ONCOTARGET |
ISSN | 1949-2553 |
卷号 | 8期号:67页码:111866-111881 |
收录类别 | SCI |
WOS关键词 | HUMAN HEPATOCELLULAR-CARCINOMA ; CHOLINE-DEVOID DIET ; ACID-DEFINED DIETS ; BREAST-CANCER ; EXPRESSION ANALYSIS ; DNA METHYLATION ; BINDING PROTEIN ; RAS ONCOGENES ; GENOME-WIDE ; IN-VITRO |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
WOS研究方向 | Oncology ; Cell Biology |
WOS类目 | Oncology ; Cell Biology |
WOS记录号 | WOS:000419567000090 |
WOS分区 | Q1 |
资助机构 | China-Canada team grant from the Canadian Institute of Health Research ; National Science Fund of China |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.psych.ac.cn/handle/311026/26031 |
专题 | 中国科学院心理健康重点实验室 |
通讯作者 | Moshe Szyf |
作者单位 | 1.Chinese Acad Sci, Inst Psychol, CAS Key Lab Mental Hlth, Beijing, Peoples R China 2.McGill Univ Montreal, Dept Pharmacol & Therapeut, Montreal, PQ, Canada 3.Chinese Acad Sci, Beijing Inst Life Sci, Beijing, Peoples R China |
第一作者单位 | 中国科学院心理健康重点实验室 |
推荐引用方式 GB/T 7714 | Wang, Yan,Sun, ZhongSheng,Szyf, Moshe,et al. S-adenosyl-methionine (SAM) alters the transcriptome and methylome and specifically blocks growth and invasiveness of liver cancer cells[J]. ONCOTARGET,2017,8(67):111866-111881. |
APA | Wang, Yan,Sun, ZhongSheng,Szyf, Moshe,&Moshe Szyf.(2017).S-adenosyl-methionine (SAM) alters the transcriptome and methylome and specifically blocks growth and invasiveness of liver cancer cells.ONCOTARGET,8(67),111866-111881. |
MLA | Wang, Yan,et al."S-adenosyl-methionine (SAM) alters the transcriptome and methylome and specifically blocks growth and invasiveness of liver cancer cells".ONCOTARGET 8.67(2017):111866-111881. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
S-adenosyl-methionin(6687KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 请求全文 |
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