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Expression profiles and functional annotation analysis of mRNAs in suprachiasmatic nucleus of Clock mutant mice
Wang, Yanli1; Lv, Ke2; Zhao, Mei3; Liang, Fengji2; Chen, Hailong2; Ji, Guohua2; Wang, Tingmei1; Zhang, Yongliang1; Cao, Hongqing2; Li, Yinghui1,2; Qu, Lina2; Y. Li; L. Qu
2018-03-20
Source PublicationGENE
Correspondent Emailyinghuidd@vip.sina.com ; linaqu@263.net (l. qu).
ISSN0378-1119
SubtypeArticle
Volume647Pages:107-114
Contribution Rank3
Abstract

The core circadian clock gene, Clock, is a positive component of the transcription/translation feedback loop in the master pacemaker suprachiasmatic nucleus (SCN) in mammals. The robust daytime peak of some clock genes in the wild-type SCN is absent in Clock mutant mice. However, very little is known about the impact of Clock mutation on the expression of other functional genes in SCN. Here, we performed cDNA microarray and found 799 differentially expressed genes (DEGs) at zeitgeber time 2 (ZT2) and 1289 DEGs at ZT14 in SCN of Clock(Delta 19/Delta 19) mutant mice. KEGG pathway analysis showed that the changed mRNAs were highly associated with hedgehog signaling pathway, retinol metabolism, allograft rejection, drug metabolism, hematopoietic cell lineage and neuroactive ligand-receptor interaction. The top 14 and 71 hub genes were identified from the protein protein interaction (PPI) network at ZT2 and ZT14, respectively. The sub-networks revealed hub genes were involved in olfactory transduction and neuroactive ligand-receptor interaction pathways. These results demonstrate the Clock(Delta 19/Delta 19) mutation alters the expression of various genes involved in a wide spectrum of biological function in mouse SCN, which are helpful for better understanding the function of Clock and potential regulatory mechanisms.

KeywordCircadian clock Suprachiasmatic nucleus Microarray Clock mutation
Subject AreaLinaqu@263.net
DOI10.1016/j.gene.2017.12.056
Indexed BySCI
Language英语
Funding OrganizationFoundation of Advanced Space Medico-Engineering Research Project of China(2015SY54A0501) ; National Major Scientific Instrument and Equipment Development Project(2013YQ19046706 ; State Key Laboratory of Space Medicine Fundamentals and Application in China(SMFA15B01 ; 2012YQ0401400901) ; SMFA13B02 ; SMFA09A06)
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000425576500013
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordNICOTINIC ACETYLCHOLINE-RECEPTORS ; CIRCADIAN-RHYTHMS ; PARKINSONS-DISEASE ; WILD-TYPE ; PATHWAY ; BRAIN ; MT1 ; MECHANISMS ; DISORDER ; BEHAVIOR
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/26039
Collection健康与遗传心理学研究室
Corresponding AuthorY. Li; L. Qu
Affiliation1.Northwestern Polytech Univ, Sch Life Sci, Xian 710072, Shaanxi, Peoples R China
2.China Astronaut Res & Training Ctr, State Key Lab Space Med Fundamentals & Applicat, Beijing 100094, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yanli,Lv, Ke,Zhao, Mei,et al. Expression profiles and functional annotation analysis of mRNAs in suprachiasmatic nucleus of Clock mutant mice[J]. GENE,2018,647:107-114.
APA Wang, Yanli.,Lv, Ke.,Zhao, Mei.,Liang, Fengji.,Chen, Hailong.,...&L. Qu.(2018).Expression profiles and functional annotation analysis of mRNAs in suprachiasmatic nucleus of Clock mutant mice.GENE,647,107-114.
MLA Wang, Yanli,et al."Expression profiles and functional annotation analysis of mRNAs in suprachiasmatic nucleus of Clock mutant mice".GENE 647(2018):107-114.
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