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Anticancer activity of safranal against colon carcinoma is due to induction of apoptosis and G2/M cell cycle arrest mediated by suppression of mTOR/PI3K/Akt pathway
Zhang, Yibing1; Zhao, Yong2; Guo, Jianyou3; Cui, Haifeng2; Liu, Sha1
2018-05-01
发表期刊JOURNAL OF BUON
ISSN1107-0625
卷号23期号:3页码:574-578
摘要Purpose: Colon cancer is among the deadliest malignancies and is responsible for a significant number of deaths across the globe. The treatment options for colon cancer are limited and with lot of side effects. In this study we evaluated the potential anticancer effects of safranal against colo-205 colon cancer cells along with evaluation of its e f fects on apoptosis, cell cycle phase distribution, reactive oxygene species (ROS) and PI3K/AKT/m-TOR signalling pathway. Methods: Cell viability was examined by MTT assay. Apoptosis was checked by DAPI staining, comet assay and annexin V/propidium iodide (PI) staining involving the use of fluorescence microscopy and flow cytometry. ROS, mitochondria) membrane potential (MMP) and cell cycle phase distribution were checked by flow cytometry using different fluorescent probes. Effects of safranal on the protein expression of PI3K/AKTim-TOR were studied by western blot assay. Results: The results revealed that safranal suppressed the proliferation of colo-205 cancer cells with an IC50 of 20 mu M. Furthermore, the anticancer effects of safranal were found to be due to accretion of ROS and decrease in the MMP, ultimately leading to apoptosis of colo-205 cancer cells. In addition, safranal caused increase in the expression of Bax in parallel with concomitant reduction in the expression of Bcl-2. Safranal also triggered G2/M cell cycle arrest and inhibition of PI3K/AKT/mTOR signalling pathway. Conclusion: In conclusion, the above results indicate that safranal could prove a potential lead molecule in the treatment of colon cancer, provided further in vivo studies are carried out.
关键词apoptosis cell cycle colon cancer ROS safranal
语种英语
WOS研究方向Oncology
WOS类目Oncology
WOS记录号WOS:000435151300005
出版者IMPRIMATUR PUBLICATIONS
关键词[WOS]CANCER
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文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/26408
专题健康与遗传心理学研究室
通讯作者Liu, Sha
作者单位1.Chongqing Med Univ, Sch Tradit Chinese Med, Chongqing 401331, Peoples R China
2.China Acad Tradit Chinese Med, Inst Chinese Materia Med, Beijing 100700, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Beijing 100101, Peoples R China
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Zhang, Yibing,Zhao, Yong,Guo, Jianyou,et al. Anticancer activity of safranal against colon carcinoma is due to induction of apoptosis and G2/M cell cycle arrest mediated by suppression of mTOR/PI3K/Akt pathway[J]. JOURNAL OF BUON,2018,23(3):574-578.
APA Zhang, Yibing,Zhao, Yong,Guo, Jianyou,Cui, Haifeng,&Liu, Sha.(2018).Anticancer activity of safranal against colon carcinoma is due to induction of apoptosis and G2/M cell cycle arrest mediated by suppression of mTOR/PI3K/Akt pathway.JOURNAL OF BUON,23(3),574-578.
MLA Zhang, Yibing,et al."Anticancer activity of safranal against colon carcinoma is due to induction of apoptosis and G2/M cell cycle arrest mediated by suppression of mTOR/PI3K/Akt pathway".JOURNAL OF BUON 23.3(2018):574-578.
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