Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model

doi:10.1016/j.brainresbull.2018.08.013

PSYCH OpenIR

	Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model
	Sun, Lan 1; Min, Li 2; Li, Man 3; Shao, Feng 1; Wang, Weiwen 4
通讯作者	Shao, Feng(shaof@pku.edu.cn) ; Wang, Weiwen(wangww@psych.ac.cn)
摘要	Complex interactions between genetic and environmental factors exert a sustained influence on the pathogenesis of schizophrenia (SCZ). Adolescent social isolation is regarded as a typical paradigm for SCZ. However, the underlying pathological mechanisms are not fully understood. In this study, adolescent Sprague-Dawley (SD) rats were placed in isolation rearing (IR) or social rearing (SR) conditions from postnatal day (PND) 21 to 34 to establish a SCZ disease model and a control model, respectively. Prepulse inhibition (PPI) assays and elevated plus maze tests were performed on PND 56. Next, prefrontal cortex (PFC) tissues were isolated for transcriptomic sequencing and RT-qPCR analyses. The results indicated that adolescent social isolation induced anxious behaviors and disrupted PPIs as well as specific PFC gene expression patterns in adult SD rats. A total of 196 genes were identified as upregulated, and 748 genes were identified as down-regulated in the IR group compared with those in the SR group. Differentially expressed genes (DEGs) were highly enriched in the KEGG pathways associated with the comorbidity of neurological disorder and oxidative phosphorylation (OXPHOS); 26 out of 27 comorbid neurological disorder-associated DEGs overlapped with 31 OXPHOS-associated DEGs. Those 26 overlapping DEGs were all upregulated in the IR group and could easily distinguish the IR group from the SR group; 6 of these DEGs (COX7C, NDUFB11, NDUFA2, NDUFC2, ATP5C1, and COX6A1) were verified by RT-qPCR. Here, we provide a systematic overview of gene expression alterations in adolescent-social-isolationinduced SCZ (ASI-SCZ), which suggests that genes that are associated with the comorbidity of neurological disorders, especially OXPHOS-related genes, contribute to the pathogenesis of ASI-SCZ.
关键词	Schizophrenia Transcriptomic analysis Adolescent social isolation Prefrontal cortex (PFC) Oxidative phosphorylation (OXPHOS) Comorbidity
	2018-09-01
语种	en
DOI	10.1016/j.brainresbull.2018.08.013
发表期刊	BRAIN RESEARCH BULLETIN
ISSN	0361-9230
卷号	142 页码:304-312
收录类别	SCI
资助项目	National Natural Science Foundation of China[31470988] ; National Natural Science Foundation of China[81471122] ; Beijing Key Laboratory of Behavior and Mental Health ; Chinese Academy of Sciences[KJZD-EW-L04] ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS关键词	PREFRONTAL CORTEX ; ADULT RATS ; LATENT INHIBITION ; SEROTONERGIC FUNCTION ; RECEPTOR EXPRESSION ; NUCLEUS-ACCUMBENS ; SCHIZOPHRENIA ; GENE ; RELEVANCE ; BEHAVIOR
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
WOS记录号	WOS:000448223400034
资助机构	National Natural Science Foundation of China ; Beijing Key Laboratory of Behavior and Mental Health ; Chinese Academy of Sciences ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences
引用统计
文献类型	期刊论文
条目标识符	https://ir.psych.ac.cn/handle/311026/27384
通讯作者	Shao, Feng; Wang, Weiwen
作者单位	1.Peking Univ, Beijing Key Lab Behav & Mental Hlth, Sch Psychol & Cognit Sci, Beijing 100871, Peoples R China 2.Capital Med Univ, Beijing Friendship Hosp, Dept Gastroenterol, Beijing 100050, Peoples R China 3.Tianjin Normal Univ, Sch Educ Sci, Dept Psychol, Tianjin 300387, Peoples R China 4.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, 16 Lincui Rd, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Sun, Lan,Min, Li,Li, Man,et al. Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model[J]. BRAIN RESEARCH BULLETIN,2018,142:304-312.
APA	Sun, Lan,Min, Li,Li, Man,Shao, Feng,&Wang, Weiwen.(2018).Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model.BRAIN RESEARCH BULLETIN,142,304-312.
MLA	Sun, Lan,et al."Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model".BRAIN RESEARCH BULLETIN 142(2018):304-312.