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Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model
Sun, Lan1; Min, Li2; Li, Man3; Shao, Feng1; Wang, Weiwen4
First AuthorSun, Lan
2018-09-01
Source PublicationBRAIN RESEARCH BULLETIN
Correspondent Emailshaof@pku.edu.cn (f. shao) ; , wangww@psych.ac.cn (w. wang)
ISSN0361-9230
SubtypeArticle
Volume142Pages:304-312
Contribution Rank4
Abstract

Complex interactions between genetic and environmental factors exert a sustained influence on the pathogenesis of schizophrenia (SCZ). Adolescent social isolation is regarded as a typical paradigm for SCZ. However, the underlying pathological mechanisms are not fully understood. In this study, adolescent Sprague-Dawley (SD) rats were placed in isolation rearing (IR) or social rearing (SR) conditions from postnatal day (PND) 21 to 34 to establish a SCZ disease model and a control model, respectively. Prepulse inhibition (PPI) assays and elevated plus maze tests were performed on PND 56. Next, prefrontal cortex (PFC) tissues were isolated for transcriptomic sequencing and RT-qPCR analyses. The results indicated that adolescent social isolation induced anxious behaviors and disrupted PPIs as well as specific PFC gene expression patterns in adult SD rats. A total of 196 genes were identified as upregulated, and 748 genes were identified as down-regulated in the IR group compared with those in the SR group. Differentially expressed genes (DEGs) were highly enriched in the KEGG pathways associated with the comorbidity of neurological disorder and oxidative phosphorylation (OXPHOS); 26 out of 27 comorbid neurological disorder-associated DEGs overlapped with 31 OXPHOS-associated DEGs. Those 26 overlapping DEGs were all upregulated in the IR group and could easily distinguish the IR group from the SR group; 6 of these DEGs (COX7C, NDUFB11, NDUFA2, NDUFC2, ATP5C1, and COX6A1) were verified by RT-qPCR. Here, we provide a systematic overview of gene expression alterations in adolescent-social-isolationinduced SCZ (ASI-SCZ), which suggests that genes that are associated with the comorbidity of neurological disorders, especially OXPHOS-related genes, contribute to the pathogenesis of ASI-SCZ.

KeywordSchizophrenia Transcriptomic Analysis Adolescent Social Isolation Prefrontal Cortex (Pfc) Oxidative Phosphorylation (Oxphos) Comorbidity
DOI10.1016/j.brainresbull.2018.08.013
Indexed BySCI
Language英语
Funding ProjectBeijing Key Laboratory of Behavior and Mental Health ; National Natural Science Foundation of China[81471122] ; National Natural Science Foundation of China[31470988] ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences ; Chinese Academy of Sciences[KJZD-EW-L04] ; Chinese Academy of Sciences[KJZD-EW-L04] ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences ; National Natural Science Foundation of China[31470988] ; National Natural Science Foundation of China[81471122] ; Beijing Key Laboratory of Behavior and Mental Health
WOS Research AreaNeurosciences & Neurology
WOS SubjectNeurosciences
WOS IDWOS:000448223400034
PublisherPERGAMON-ELSEVIER SCIENCE LTD
WOS KeywordPrefrontal Cortex ; Adult Rats ; Latent Inhibition ; Serotonergic Function ; Receptor Expression ; Nucleus-accumbens ; Schizophrenia ; Gene ; Relevance ; Behavior
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/27386
Collection中国科学院心理健康重点实验室
Corresponding AuthorShao, Feng; Wang, Weiwen
Affiliation1.Peking Univ, Beijing Key Lab Behav & Mental Hlth, Sch Psychol & Cognit Sci, Beijing 100871, Peoples R China
2.Capital Med Univ, Beijing Friendship Hosp, Dept Gastroenterol, Beijing 100050, Peoples R China
3.Tianjin Normal Univ, Sch Educ Sci, Dept Psychol, Tianjin 300387, Peoples R China
4.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, 16 Lincui Rd, Beijing 100101, Peoples R China
Corresponding Author AffilicationKey Laboratory of Mental Health, CAS
Recommended Citation
GB/T 7714
Sun, Lan,Min, Li,Li, Man,et al. Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model[J]. BRAIN RESEARCH BULLETIN,2018,142:304-312.
APA Sun, Lan,Min, Li,Li, Man,Shao, Feng,&Wang, Weiwen.(2018).Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model.BRAIN RESEARCH BULLETIN,142,304-312.
MLA Sun, Lan,et al."Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model".BRAIN RESEARCH BULLETIN 142(2018):304-312.
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