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Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model
Sun, Lan1; Min, Li2; Li, Man3; Shao, Feng1; Wang, Weiwen4
第一作者Sun, Lan
通讯作者邮箱shaof@pku.edu.cn (f. shao) ; wangww@psych.ac.cn (w. wang)
心理所单位排序4
摘要

Complex interactions between genetic and environmental factors exert a sustained influence on the pathogenesis of schizophrenia (SCZ). Adolescent social isolation is regarded as a typical paradigm for SCZ. However, the underlying pathological mechanisms are not fully understood. In this study, adolescent Sprague-Dawley (SD) rats were placed in isolation rearing (IR) or social rearing (SR) conditions from postnatal day (PND) 21 to 34 to establish a SCZ disease model and a control model, respectively. Prepulse inhibition (PPI) assays and elevated plus maze tests were performed on PND 56. Next, prefrontal cortex (PFC) tissues were isolated for transcriptomic sequencing and RT-qPCR analyses. The results indicated that adolescent social isolation induced anxious behaviors and disrupted PPIs as well as specific PFC gene expression patterns in adult SD rats. A total of 196 genes were identified as upregulated, and 748 genes were identified as down-regulated in the IR group compared with those in the SR group. Differentially expressed genes (DEGs) were highly enriched in the KEGG pathways associated with the comorbidity of neurological disorder and oxidative phosphorylation (OXPHOS); 26 out of 27 comorbid neurological disorder-associated DEGs overlapped with 31 OXPHOS-associated DEGs. Those 26 overlapping DEGs were all upregulated in the IR group and could easily distinguish the IR group from the SR group; 6 of these DEGs (COX7C, NDUFB11, NDUFA2, NDUFC2, ATP5C1, and COX6A1) were verified by RT-qPCR. Here, we provide a systematic overview of gene expression alterations in adolescent-social-isolationinduced SCZ (ASI-SCZ), which suggests that genes that are associated with the comorbidity of neurological disorders, especially OXPHOS-related genes, contribute to the pathogenesis of ASI-SCZ.

关键词Schizophrenia Transcriptomic Analysis Adolescent Social Isolation Prefrontal Cortex (Pfc) Oxidative Phosphorylation (Oxphos) Comorbidity
2018-09-01
语种英语
DOI10.1016/j.brainresbull.2018.08.013
发表期刊BRAIN RESEARCH BULLETIN
ISSN0361-9230
卷号142页码:304-312
收录类别SCI ; SSCI
资助项目Beijing Key Laboratory of Behavior and Mental Health ; National Natural Science Foundation of China[81471122] ; National Natural Science Foundation of China[31470988] ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences ; Chinese Academy of Sciences[KJZD-EW-L04] ; Chinese Academy of Sciences[KJZD-EW-L04] ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences ; National Natural Science Foundation of China[31470988] ; National Natural Science Foundation of China[81471122] ; Beijing Key Laboratory of Behavior and Mental Health
出版者PERGAMON-ELSEVIER SCIENCE LTD
WOS关键词Prefrontal Cortex ; Adult Rats ; Latent Inhibition ; Serotonergic Function ; Receptor Expression ; Nucleus-accumbens ; Schizophrenia ; Gene ; Relevance ; Behavior
WOS研究方向Neurosciences & Neurology
WOS类目Neurosciences
WOS记录号WOS:000448223400034
WOS分区Q2
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/27386
专题中国科学院心理健康重点实验室
通讯作者Shao, Feng; Wang, Weiwen
作者单位1.Peking Univ, Beijing Key Lab Behav & Mental Hlth, Sch Psychol & Cognit Sci, Beijing 100871, Peoples R China
2.Capital Med Univ, Beijing Friendship Hosp, Dept Gastroenterol, Beijing 100050, Peoples R China
3.Tianjin Normal Univ, Sch Educ Sci, Dept Psychol, Tianjin 300387, Peoples R China
4.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, 16 Lincui Rd, Beijing 100101, Peoples R China
通讯作者单位中国科学院心理健康重点实验室
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GB/T 7714
Sun, Lan,Min, Li,Li, Man,et al. Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model[J]. BRAIN RESEARCH BULLETIN,2018,142:304-312.
APA Sun, Lan,Min, Li,Li, Man,Shao, Feng,&Wang, Weiwen.(2018).Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model.BRAIN RESEARCH BULLETIN,142,304-312.
MLA Sun, Lan,et al."Transcriptomic analysis reveals oxidative phosphorylation activation in an adolescent social isolation rat model".BRAIN RESEARCH BULLETIN 142(2018):304-312.
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