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MiR-155 affects osteosarcoma cell proliferation and invasion through regulating NF-kappa B signaling pathway
Lu, S.1; Liao, Q-S2; Tang, L.1
First AuthorLu, S.
2018-11-01
Source PublicationEUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
Correspondent Emailf135155797999113@sina.com
ISSN1128-3602
SubtypeArticle
Volume22Issue:22Pages:7633-7639
Contribution Rank2
Abstract

OBJECTIVE: Osteosarcoma can form tumor osteoid tissue and bone tissue directly or indirectly through cartilage stage. It mainly occurs in children and adolescents with high mortality. MicroRNA (miRNA) possesses tissue sensitivity as tumor biomarker and plays a promoting or inhibitory role in tumor pathogenesis as oncogene or tumor suppressor gene. It was found that miR-155 was abnormally expressed in tumor and could be treated as a biomarker for cancer progression. However, miR-155 expression in osteosarcoma and related mechanism still remains unclear. This study aimed to explore the role of miR-155 in osteosarcoma occurrence and development. MATERIALS AND METHODS: Osteosarcoma cell line MG-63 was cultured in vitro and transfected by miR-155 mimics or inhibitor. MiR-155 expression was examined by Real-time PCR (RT-PCR). Cell proliferation was evaluated by MTT assay. Caspase 3 activity was determined by caspase 3 activity detection kit. Cell invasion was measured by transwell assay. B-cell lymphoma-2 (Bcl-2) and nuclear factor kappa B (NF-kappa B) protein expressions were assessed by Western blot. RESULTS: MiR-155 mimics significantly up-regulated miR-155 expression, promoted MG-63 cell proliferation and invasion, inhibited caspase 3 activity, and up-regulated expressions of NF-kappa B and Bcl-2 compared with control group (p < 0.05). However, miR-155 inhibitor significantly inhibited MG-63 cell proliferation and invasion, enhanced caspase 3 activity, and reduced expressions of NF-kappa B and Bcl-2 compared with control group (p < 0.05). CONCLUSIONS: MiR-155 affected osteosarcoma cell proliferation and apoptosis through regulating NF-kappa B signaling pathway, indicating it might be a new biomarker for osteosarcoma diagnosis and treatment.

KeywordMir-155 Nf-kappa b Osteosarcoma Proliferation Invasion
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000452774900005
PublisherVERDUCI PUBLISHER
WOS KeywordLung-cancer ; Metastasis ; Expression ; Growth
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/27950
Collection健康与遗传心理学研究室
Corresponding AuthorTang, L.
Affiliation1.Shenzhen Univ, Hlth Sci Ctr, Coll Optoelect Engn, Shenzhen, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Psychol, Shenzhen, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Lu, S.,Liao, Q-S,Tang, L.. MiR-155 affects osteosarcoma cell proliferation and invasion through regulating NF-kappa B signaling pathway[J]. EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,2018,22(22):7633-7639.
APA Lu, S.,Liao, Q-S,&Tang, L..(2018).MiR-155 affects osteosarcoma cell proliferation and invasion through regulating NF-kappa B signaling pathway.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,22(22),7633-7639.
MLA Lu, S.,et al."MiR-155 affects osteosarcoma cell proliferation and invasion through regulating NF-kappa B signaling pathway".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 22.22(2018):7633-7639.
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