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Simultaneous Recordings of Cortical Local Field Potentials and Electrocorticograms in Response to Nociceptive Laser Stimuli from Freely Moving Rats
Yue, Lupeng1,2; Zhang, Fengrui3; Lu, Xuejing1,2; Wan, You4,5,6; Hu, Li1,2,7
First AuthorLupeng Yue
2019
Source PublicationJOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Correspondent Emailyou wan at ywan@hsc.pku.edu.cn ; li hu at huli@psych.ac.cn
ISSN1940-087X
SubtypeArticle
Issue143Pages:7
Contribution Rank1
Abstract

Electrocortical responses, elicited by laser heat pulses that selectively activate nociceptive free nerve endings, are widely used in many animal and human studies to investigate the cortical processing of nociceptive information. These laser-evoked brain potentials (LEPs) consist of several transient responses that are time-locked to the onset of laser stimuli. However, the functional properties of the LEP responses are still largely unknown, due to the lack of a sampling technique that can simultaneously record neural activities at the surface of the cortex (i.e., electrocorticogram [ECoG] and scalp electroencephalogram [scalp EEG]) and inside the brain (i.e., local field potential [LFP]). To address this issue, we present here an animal protocol using freely moving rats. This protocol is composed of three main procedures: (1) animal preparation and surgical procedures, (2) a simultaneous recording of ECoG and LFP in response to nociceptive laser stimuli, and (3) data analysis and feature extraction. Specifically, with the help of a 3D-printed protective shell, both ECoG and LFP electrodes implanted on the rat's skull were securely held together. During data collection, laser pulses were delivered on the rat's forepaws through gaps in the bottom of the chamber when the animal was in spontaneous stillness. Ongoing white noise was played to avoid the activation of the auditory system by the laser-generated ultrasounds. As a consequence, only nociceptive responses were selectively recorded. Using the standard analytical procedures (e.g., band-pass filtering, epoch extraction, and baseline correction) to extract stimulus-related brain responses, we obtained results showing that LEPs with a high signal-to-noise ratio were simultaneously recorded from ECoG and LFP electrodes. This methodology makes the simultaneous recording of ECoG and LFP activities possible, which provides a bridge of electrocortical signals at the mesoscopic and macroscopic levels, thereby facilitating the investigation of nociceptive information processing in the brain.

KeywordBehavior Issue 143 Electroencephalogram (EEG) electrocorticogram (ECoG) local field potential (LFP) laser-evoked potential (LEP) pain animal model simultaneous recording
DOI10.3791/58686
Indexed BySCI
Language英语
Funding ProjectScientific Foundation project of the Institute of Psychology, Chinese Academy of Sciences[Y6CX021008] ; 13th Five-year Informatization Plan of the Chinese Academy of Sciences[XXH13506] ; National Natural Science Foundation of China[31822025] ; CAS Key Laboratory of Mental Health, Institute of Psychology ; National Natural Science Foundation of China[31671141]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000458012000099
PublisherJOURNAL OF VISUALIZED EXPERIMENTS
WOS KeywordPRIMARY SOMATOSENSORY CORTEX ; COMPONENTS ; PATHWAYS ; NEURONS ; PAIN ; EEG ; N1
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/28320
Collection中国科学院心理健康重点实验室
Corresponding AuthorWan, You; Hu, Li
Affiliation1.CAS Key Lab Mental Hlth, Inst Psychol, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Dept Psychol, Beijing, Peoples R China
3.Liaoning Normal Univ, Res Ctr Brain Cognit Neurosci, Dalian, Peoples R China
4.Peking Univ, Neurosci Res Inst, Beijing, Peoples R China
5.Peking Univ, Sch Basic Med Sci, Dept Neurobiol, Beijing, Peoples R China
6.Peking Univ, Minist Educ, Natl Hlth & Family Planning Commiss, Key Lab Neurosci, Beijing, Peoples R China
7.Guangzhou Med Univ, Affiliated Hosp 2, State Key Clin Specialty Pain Med, Dept Pain Management, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Yue, Lupeng,Zhang, Fengrui,Lu, Xuejing,et al. Simultaneous Recordings of Cortical Local Field Potentials and Electrocorticograms in Response to Nociceptive Laser Stimuli from Freely Moving Rats[J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,2019(143):7.
APA Yue, Lupeng,Zhang, Fengrui,Lu, Xuejing,Wan, You,&Hu, Li.(2019).Simultaneous Recordings of Cortical Local Field Potentials and Electrocorticograms in Response to Nociceptive Laser Stimuli from Freely Moving Rats.JOVE-JOURNAL OF VISUALIZED EXPERIMENTS(143),7.
MLA Yue, Lupeng,et al."Simultaneous Recordings of Cortical Local Field Potentials and Electrocorticograms in Response to Nociceptive Laser Stimuli from Freely Moving Rats".JOVE-JOURNAL OF VISUALIZED EXPERIMENTS .143(2019):7.
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