mGluR5 in the nucleus accumbens shell regulates morphine-associated contextual memory through reactive oxygen species signaling | |
Chong Qi; Xinjuan Wang; Feifei Ge; Yijing Li; Fang Shen; Junkai Wang; Cailian Cui | |
第一作者 | Chong Qi ; Xinjuan Wang |
通讯作者邮箱 | cui, c (clcui@bjmu.edu.cn) |
摘要 | Emerging evidence indicates that metabotropic glutamate receptor 5 (mGluR5) critically modulates drug and drug-related behaviors. However, the role of mGluR5 in the opiate-induced contextual memory remains unclear. Here, we found that microinfusion of the mGluR5 antagonist 3-((2-Methyl-1,3-thiazol-4-yl)ethynyl)pyridine (MTEP) into the nucleus accumbens (NAc) shell, but not into the core, significantly attenuated the expression of morphine conditioned place preference (CPP) in rats. Following the expression of morphine CPP, the protein level of membrane mGluR5 was selectively increased in the NAc shell. In primary striatal neurons, we observed that treatment with the mGluR5 agonist CHPG increased the phosphorylation level of extracellular signal-regulated kinase (ERK), which was dependent on the mGluR5-inositol-1,4,5-trisphosphate-reactive oxygen species (ROS) pathway. Moreover, the microinjection of the ROS scavenger Tempol into the NAc shell of rats blocked the expression of morphine CPP. Further, the administration of t-BOOH, a ROS donor, into the NAc shell rescued the retrieval impairment of morphine CPP produced by MTEP. Our previous study demonstrated that the expression of morphine CPP increased the phosphorylation of ERK selectively in the NAc shell. Thus, results of the present study suggest that mGluR5 in the NAc shell, but not in the core, is essential for the retrieval of morphine contextual memory, which is mediated at least in part, through the ROS/ERK signaling pathway. Uncovering the molecular basis of opiate contextual memory will benefit the development of new therapeutic approaches for the treatment of opiate addiction. |
关键词 | Conditioned place preference (CPP) metabotropic glutamate receptor 5 (mGluR5) morphine nucleus accumbens (NAc) reactive oxygen species (ROS) retrieval |
2015 | |
语种 | 英语 |
DOI | 10.1111/adb.12222 |
发表期刊 | Addiction Biology |
ISSN | 1355-6215 |
卷号 | 20期号:5页码:927–940 |
期刊论文类型 | Article |
收录类别 | SCI |
Q分类 | Q1 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.psych.ac.cn/handle/311026/29166 |
专题 | 健康与遗传心理学研究室 |
通讯作者 | Cailian Cui |
作者单位 | 1.Peking Univ, Minist Educ, Key Lab Neurosci, Dept Neurobiol,Sch Basic Med Sci, Beijing 2.Peking Univ, Neurosci Res Inst, Natl Hlth & Family Planning Commiss, Beijing |
推荐引用方式 GB/T 7714 | Chong Qi,Xinjuan Wang,Feifei Ge,et al. mGluR5 in the nucleus accumbens shell regulates morphine-associated contextual memory through reactive oxygen species signaling[J]. Addiction Biology,2015,20(5):927–940. |
APA | Chong Qi.,Xinjuan Wang.,Feifei Ge.,Yijing Li.,Fang Shen.,...&Cailian Cui.(2015).mGluR5 in the nucleus accumbens shell regulates morphine-associated contextual memory through reactive oxygen species signaling.Addiction Biology,20(5),927–940. |
MLA | Chong Qi,et al."mGluR5 in the nucleus accumbens shell regulates morphine-associated contextual memory through reactive oxygen species signaling".Addiction Biology 20.5(2015):927–940. |
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文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
mGluR5 in the nucleu(1354KB) | 期刊论文 | 作者接受稿 | 限制开放 | CC BY-NC-SA | 请求全文 |
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