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维甲酸诱导小鼠胚胎脊柱裂畸形豹全基因表达分析研究
Alternative TitleGlobal gene expression analysis of embryos with spina bifida after maternal exposure to Retinoic acid
刘帅
Subtype硕士
Thesis Advisor孙中生
2011-06
Degree Grantor中国科学院大学
Place of Conferral中国科学院心理研究所
Degree Name理学硕士
Degree Discipline行为遗传学
Keyword维甲酸 脊柱裂 基因表达 微阵列芯片 小鼠
Abstract

维生素A衍生物维甲酸(retinoic acid, RA)是可以诱导包括神经管畸形在内的多种先天畸形有效致畸剂。维甲酸是维生素A在体内的自然活性代谢物,也是胚胎发育过程中不可缺少的形态发生素,已有研究资料提示维甲酸在机体发育及维持机体正常生理功能方面均有重要作用,可以影响器官生成,发育和神经管的闭合过程,但具体机制尚不清楚。本论文实验结合微阵列芯片技术,在小鼠脊柱裂畸形的脊髓组织样本和正常对照组比较发现180个基因存在l.5倍以上差异。基因富集分析显示孕期母鼠暴露于维甲酸诱导的胎鼠脊柱裂畸形与多基因的改变相关,这些基因主要作用包括促进细胞凋亡、抗细胞凋亡、细胞分化和汗移、细胞骨架构建、细胞粘附或者勃着斑的形成,这也表明了正是这些细胞组.分和相应生物过程功能的缺陷导致神经管的不正常发育。本一论文研究对维甲酸诱导小鼠胚胎脊柱裂畸形进行了全基因表达分析研究,有利于对神经管畸形病因病理学进行更进一步的了解和研究。

Other Abstract

The vitamin A derivative, retinoic acid (RA), is a potent teratogenic agent that induces a variety of congenital abnormalities including neural tube defects. The embryopathology of RA has been extensively investigated and retinol receptors play important roles during organogenesis, development and neural tube closure. Still; the mechanisms by which RA influences these processes are not incompletely understood. Using microarray technology, the present study identifies 180 genes in the spinal cord that exhibit at least a 1.5-fold change between control samples and samples with spine bifida aperta. A gene set enrichment analysis showed that maternal exposure to RA induced spinal bifida that were associated with altered expression of gene involved in pro- or anti-apoptosis, cell proliferation, migration, cytoskeleton components, and cell or focal adhesion, indicating that defectives functions of these cell components and biological processes preceded the abnormal development of neural tube. Our study provides a global analysis of gene expression patterns in spine bifida and will help to the understanding of the etiology and pathology of neural tube defects.

Pages36
Language中文
Document Type学位论文
Identifierhttp://ir.psych.ac.cn/handle/311026/29196
Collection健康与遗传心理学研究室
Recommended Citation
GB/T 7714
刘帅. 维甲酸诱导小鼠胚胎脊柱裂畸形豹全基因表达分析研究[D]. 中国科学院心理研究所. 中国科学院大学,2011.
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