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D-Ribose contributes to the glycation of serum protein
Chen, Yao1; Yu, Lexiang2; Wang, Yujing2; Wei, Yan2,4; Xu, Yong5; He, Tao1; He, Rongqiao1,2,3
First AuthorChen, Yao
2019-09-01
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Correspondent Emailherq@ibp.ac.cn (r. he)
ISSN0925-4439
Subtypearticle
Volume1865Issue:9Pages:2285-2292
Contribution Rank4
Abstract

D-Ribose is active in glycation and rapidly produces advanced glycation end products, leading to cell death and to cognitive impairment in mice. Glycated serum protein (GSP) is a relatively short-term biomarker for glycemic control in diabetes mellitus. However, whether D-ribose is related to GSP is unclear. The aim of this work was to identify the contribution of D-ribose to GSP compared to D-glucose. Here, we showed that the yield of glycated human serum albumin with D-ribose was at least two-fold higher than that with D-glucose in a 2-week incubation. The glycation of human serum albumin (HSA) with D-ribose was much faster than that with D-glucose, as determined by monitoring changes in the fluorescent intensity of glycation products with time. Liquid chromatography-mass spectrometry/mass spectrometry revealed that 17 and 7 lysine residues on HSA were glycated in the presence of D-ribose and D-glucose, respectively, even when the concentration ratio [D-ribose]/[D-glucose] was 1/50. The intraperitoneal injection of D-ribose significantly increased the GSP levels in Sprague Dawley rats, but the injection of D-glucose did not. The level of D-ribose was more positively associated with GSP than the level of D-glucose in streptozotocin-treated rats. In diabetic patients, the levels of both D-ribose and D-glucose were closely related to the level of GSP. Together, these in vitro and in vivo findings indicated that D-ribose is an important contributor to the glycation of serum protein, compared to D-glucose. To assess GSP levels in diabetes mellitus, we should consider the contribution from D-ribose, which plays a nonnegligible role.

KeywordD-Ribose Glycated serum protein D-Glucose Human serum albumin Diabetes mellitus Advanced glycation end products
DOI10.1016/j.bbadis.2019.05.005
Indexed BySCI
Language英语
Funding OrganizationNational Key Research and Development Program of China ; Beijing Municipal Science and Technology Project ; Natural Science Foundation of China NSFC ; Youth Innovation Promotion Association CAS
Funding ProjectNational Key Research and Development Program of China[2016YFC1305900] ; National Key Research and Development Program of China[2016YFC1306300] ; Beijing Municipal Science and Technology Project[Z161100000217141] ; Beijing Municipal Science and Technology Project[Z161100000216137] ; Natural Science Foundation of China NSFC[31670805] ; Natural Science Foundation of China NSFC[81573763] ; Youth Innovation Promotion Association CAS[2017132]
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS IDWOS:000476965200018
PublisherELSEVIER
WOS KeywordTYPE-2 DIABETES-MELLITUS ; END-PRODUCTS AGES ; GLYCEMIC CONTROL ; TAU HYPERPHOSPHORYLATION ; CARDIOVASCULAR OUTCOMES ; ALBUMIN ; COMPLICATIONS ; GLUCOSE ; INDICATOR ; SUGARS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/29573
Collection中国科学院心理健康重点实验室
Corresponding AuthorHe, Rongqiao
Affiliation1.Southwest Med Univ, Sch Basic Med Sci, Luzhou 646000, Sichuan, Peoples R China
2.Univ Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, 15 Datun Rd, Beijing 100101, Peoples R China
3.Capital Med Univ, Beijing Inst Brain Disorders, Alzheimers Dis Ctr, Beijing 100101, Peoples R China
4.Chinese Acad Sci, Inst Psychol, CAS Key Lab Mental Hlth, Beijing 100101, Peoples R China
5.Southwest Med Univ, Affiliated Hosp, Luzhou 646000, Peoples R China
Recommended Citation
GB/T 7714
Chen, Yao,Yu, Lexiang,Wang, Yujing,et al. D-Ribose contributes to the glycation of serum protein[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,2019,1865(9):2285-2292.
APA Chen, Yao.,Yu, Lexiang.,Wang, Yujing.,Wei, Yan.,Xu, Yong.,...&He, Rongqiao.(2019).D-Ribose contributes to the glycation of serum protein.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,1865(9),2285-2292.
MLA Chen, Yao,et al."D-Ribose contributes to the glycation of serum protein".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 1865.9(2019):2285-2292.
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