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Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration; Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration | |
Li, Xuan1; Davis, Ian R.1; Lofaro, Olivia M.2; Zhang, Jianjun3; Cimbro, Raffaello4; Rubio, F. Javier2 | |
第一作者 | Li, Xuan ; Li, Xuan |
通讯作者邮箱 | annali@umd.edu ; annali@umd.edu |
心理所单位排序 | 3 ; 3 |
摘要 | Background Methamphetamine (Meth) seeking progressively increases after withdrawal (incubation of Meth craving). We previously demonstrated a role of anterior intralaminar nucleus of thalamus (AIT) to dorsomedial striatum (DMS) projections in this incubation. Here, we examined molecular alterations in DMS and AIT neurons activated (identified by neuronal activity marker Fos) during "incubated" Meth-seeking relapse test after prolonged withdrawal. Methods We trained male rats to self-administer Meth or saline (control condition) for 10 days (6 hr/day). Using fluorescence-activated cell sorting, we examined gene expression in Fos-positive (activated during a 2-hr relapse test) and Fos-negative (nonactivated) DMS and AIT neurons. Results In DMS, we found increased mRNA expressions of immediate early genes (IEGs) (Arc, Egr1, Npas4, Fosb), Trkb, glutamate receptors subunits (Gria3, Grin1, Grin2b, Grm1), and epigenetic enzymes (Hdac3, Hdac5, Crebbp) in Fos-positive neurons, compared with Fos-negative neurons. In AIT, we found that fewer genes (Egr1, Fosb, TrkB, Grin1, and Hdac5) exhibited increased mRNA expression in Fos-positive neurons. Unexpectedly, in both brain regions, gene alterations described above also occurred in drug-naive saline self-administration control rats. Conclusions These results demonstrated that transcriptional regulations in Fos-positive neurons activated during the relapse tests are brain region-specific but are not uniquely associated with drug exposure during the self-administration training. ;Background Methamphetamine (Meth) seeking progressively increases after withdrawal (incubation of Meth craving). We previously demonstrated a role of anterior intralaminar nucleus of thalamus (AIT) to dorsomedial striatum (DMS) projections in this incubation. Here, we examined molecular alterations in DMS and AIT neurons activated (identified by neuronal activity marker Fos) during "incubated" Meth-seeking relapse test after prolonged withdrawal. Methods We trained male rats to self-administer Meth or saline (control condition) for 10 days (6 hr/day). Using fluorescence-activated cell sorting, we examined gene expression in Fos-positive (activated during a 2-hr relapse test) and Fos-negative (nonactivated) DMS and AIT neurons. Results In DMS, we found increased mRNA expressions of immediate early genes (IEGs) (Arc, Egr1, Npas4, Fosb), Trkb, glutamate receptors subunits (Gria3, Grin1, Grin2b, Grm1), and epigenetic enzymes (Hdac3, Hdac5, Crebbp) in Fos-positive neurons, compared with Fos-negative neurons. In AIT, we found that fewer genes (Egr1, Fosb, TrkB, Grin1, and Hdac5) exhibited increased mRNA expression in Fos-positive neurons. Unexpectedly, in both brain regions, gene alterations described above also occurred in drug-naive saline self-administration control rats. Conclusions These results demonstrated that transcriptional regulations in Fos-positive neurons activated during the relapse tests are brain region-specific but are not uniquely associated with drug exposure during the self-administration training. |
关键词 | anterior intralaminar nucleus of thalamus dorsal striatum fluorescence-activated cell sorting Fos incubation of drug craving methamphetamine relapse anterior intralaminar nucleus of thalamus dorsal striatum fluorescence-activated cell sorting Fos incubation of drug craving methamphetamine relapse |
2019-07-31 ; 2019-07-31 | |
语种 | 英语 ; 英语 |
DOI | 10.1002/brb3.1378 ; 10.1002/brb3.1378 |
发表期刊 | BRAIN AND BEHAVIOR ; BRAIN AND BEHAVIOR |
ISSN | 2162-3279 ; 2162-3279 |
页码 | 12 |
期刊论文类型 | article ; article |
收录类别 | SCI ; SCI |
资助项目 | Department of Psychology at University of Maryland ; Department of Psychology at University of Maryland ; NIH/NIDA[R00DA041350-02] ; NIH/NIDA[R00DA041350-02] ; National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institute of Health[P30AR053503] ; National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institute of Health[P30AR053503] |
出版者 | WILEY ; WILEY |
WOS关键词 | CONTEXT-INDUCED REINSTATEMENT ; MESSENGER-RNA EXPRESSION ; NUCLEUS-ACCUMBENS ; MOLECULAR ALTERATIONS ; DORSOMEDIAL STRIATUM ; PREFRONTAL CORTEX ; INDUCED RELAPSE ; INCUBATION ; COCAINE ; SEEKING ; CONTEXT-INDUCED REINSTATEMENT ; MESSENGER-RNA EXPRESSION ; NUCLEUS-ACCUMBENS ; MOLECULAR ALTERATIONS ; DORSOMEDIAL STRIATUM ; PREFRONTAL CORTEX ; INDUCED RELAPSE ; INCUBATION ; COCAINE ; SEEKING |
WOS研究方向 | Behavioral Sciences ; Neurosciences & Neurology ; Behavioral Sciences ; Neurosciences & Neurology |
WOS类目 | Behavioral Sciences ; Neurosciences ; Behavioral Sciences ; Neurosciences |
WOS记录号 | WOS:000479559900001 ; WOS:000479559900001 |
资助机构 | Department of Psychology at University of Maryland ; Department of Psychology at University of Maryland ; NIH/NIDA ; NIH/NIDA ; National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institute of Health ; National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institute of Health |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.psych.ac.cn/handle/311026/29879 |
专题 | 中国科学院心理健康重点实验室 |
通讯作者 | Li, Xuan |
作者单位 | 1.Univ Maryland, Dept Psychol, College Pk, MD 20742 USA 2.NIDA, Intramural Res Program, NIH, DHHS, Baltimore, MD USA 3.Inst Psychol, CAS Key Lab Mental Hlth, Beijing, Peoples R China 4.Johns Hopkins Univ, Sch Med, Div Rheumatol, Baltimore, MD USA |
推荐引用方式 GB/T 7714 | Li, Xuan,Davis, Ian R.,Lofaro, Olivia M.,et al. Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration, Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration[J]. BRAIN AND BEHAVIOR, BRAIN AND BEHAVIOR,2019, 2019:12, 12. |
APA | Li, Xuan,Davis, Ian R.,Lofaro, Olivia M.,Zhang, Jianjun,Cimbro, Raffaello,&Rubio, F. Javier.(2019).Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration.BRAIN AND BEHAVIOR,12. |
MLA | Li, Xuan,et al."Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration".BRAIN AND BEHAVIOR (2019):12. |
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