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Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration
Li, Xuan1; Davis, Ian R.1; Lofaro, Olivia M.2; Zhang, Jianjun3; Cimbro, Raffaello4; Rubio, F. Javier2
First AuthorLi, Xuan
2019-07-31
Source PublicationBRAIN AND BEHAVIOR
Correspondent Emailannali@umd.edu
ISSN2162-3279
Subtypearticle
Pages12
Contribution Rank3
Abstract

Background Methamphetamine (Meth) seeking progressively increases after withdrawal (incubation of Meth craving). We previously demonstrated a role of anterior intralaminar nucleus of thalamus (AIT) to dorsomedial striatum (DMS) projections in this incubation. Here, we examined molecular alterations in DMS and AIT neurons activated (identified by neuronal activity marker Fos) during "incubated" Meth-seeking relapse test after prolonged withdrawal. Methods We trained male rats to self-administer Meth or saline (control condition) for 10 days (6 hr/day). Using fluorescence-activated cell sorting, we examined gene expression in Fos-positive (activated during a 2-hr relapse test) and Fos-negative (nonactivated) DMS and AIT neurons. Results In DMS, we found increased mRNA expressions of immediate early genes (IEGs) (Arc, Egr1, Npas4, Fosb), Trkb, glutamate receptors subunits (Gria3, Grin1, Grin2b, Grm1), and epigenetic enzymes (Hdac3, Hdac5, Crebbp) in Fos-positive neurons, compared with Fos-negative neurons. In AIT, we found that fewer genes (Egr1, Fosb, TrkB, Grin1, and Hdac5) exhibited increased mRNA expression in Fos-positive neurons. Unexpectedly, in both brain regions, gene alterations described above also occurred in drug-naive saline self-administration control rats. Conclusions These results demonstrated that transcriptional regulations in Fos-positive neurons activated during the relapse tests are brain region-specific but are not uniquely associated with drug exposure during the self-administration training.

Keywordanterior intralaminar nucleus of thalamus dorsal striatum fluorescence-activated cell sorting Fos incubation of drug craving methamphetamine relapse
DOI10.1002/brb3.1378
Indexed BySCI
Language英语
Funding OrganizationDepartment of Psychology at University of Maryland ; NIH/NIDA ; National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institute of Health
Funding ProjectDepartment of Psychology at University of Maryland ; NIH/NIDA[R00DA041350-02] ; National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institute of Health[P30AR053503]
WOS Research AreaBehavioral Sciences ; Neurosciences & Neurology
WOS SubjectBehavioral Sciences ; Neurosciences
WOS IDWOS:000479559900001
PublisherWILEY
WOS KeywordCONTEXT-INDUCED REINSTATEMENT ; MESSENGER-RNA EXPRESSION ; NUCLEUS-ACCUMBENS ; MOLECULAR ALTERATIONS ; DORSOMEDIAL STRIATUM ; PREFRONTAL CORTEX ; INDUCED RELAPSE ; INCUBATION ; COCAINE ; SEEKING
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Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/29879
Collection中国科学院心理健康重点实验室
Corresponding AuthorLi, Xuan
Affiliation1.Univ Maryland, Dept Psychol, College Pk, MD 20742 USA
2.NIDA, Intramural Res Program, NIH, DHHS, Baltimore, MD USA
3.Inst Psychol, CAS Key Lab Mental Hlth, Beijing, Peoples R China
4.Johns Hopkins Univ, Sch Med, Div Rheumatol, Baltimore, MD USA
Recommended Citation
GB/T 7714
Li, Xuan,Davis, Ian R.,Lofaro, Olivia M.,et al. Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration[J]. BRAIN AND BEHAVIOR,2019:12.
APA Li, Xuan,Davis, Ian R.,Lofaro, Olivia M.,Zhang, Jianjun,Cimbro, Raffaello,&Rubio, F. Javier.(2019).Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration.BRAIN AND BEHAVIOR,12.
MLA Li, Xuan,et al."Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration".BRAIN AND BEHAVIOR (2019):12.
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