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Alantolactone exhibits selective antitumor effects in HELA human cervical cancer cells by inhibiting cell migration and invasion, G2/M cell cycle arrest, mitochondrial mediated apoptosis and targeting Nf-kB signalling pathway
Yibing Zhang1; Zhao, Yong2; Ran, Yongwang3; Guo, Jianyou4; Cui, Haifeng2; Liu, Sha1
First AuthorYibing Zhang
Correspondent Emailstevelmartinrr222@yahoo.com
2019-11-01
Source PublicationJOURNAL OF BUON
ISSN1107-0625
Subtypearticle
Volume24Issue:6Pages:2310-2315
Contribution Rank4
Abstract

Purpose: Cervical cancer is responsible for significant mortality and morbidity across the globe. Owing to the adverse effects of the currently used chemotherapy, research is directed to develop effective and safer chemotherapy for cervical cancer. This study was therefore designed to examine the effects of Alantolactone (AL) against a panel of human cervical cancer cells (HeLa, C4-1, MEK-180, C33A) and a normal cell line (HCerEPiC). Methods: Cell viability was examined by WST-1 assay. Cell migration and invasion were examined by transwell assay. Cell cycle analysis was performed by flow cytometry. Apoptosis was detected by annexin V/propidium iodide (PI) and DAPI staining. Western blot analysis was used to examine protein expression. Results: The results revealed that AL inhibits the growth of the all the cervical cancer cells in a concentration -dependent manner and exhibited the lowest IC50 of 15 mu M against the HeLa cervical cancer cells. The anticancer effects of AL were due to induction of mitochondrial mediated apoptosis. AL caused enhancement in the expression of apoptosis regulatory proteins such as caspase 3 and Bax in cervical cancer cells. AL not only prompted the accumulation of the cervical cancer cells in the G2/M phase of the cell cycle but also inhibited the expressions of various cyclins. Transwell assay revealed that AL suppresses the migration and invasion of cervical cancer cells. Moreover AL could also block the NF-kB signalling pathway concentration-dependently. Conclusions: It is concluded that AL may serve as an important lead molecule for the development of therapy for cervical cancer.

Keywordalantolactone apoptosis cervical cancer cytometry cell cycle
Indexed BySCI
Language英语
WOS Research AreaOncology
WOS SubjectOncology
WOS IDWOS:000503405400015
PublisherIMPRIMATUR PUBLICATIONS
WOS KeywordNATURAL-PRODUCTS ; WOMEN
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/30558
Collection健康与遗传心理学研究室
Corresponding AuthorLiu, Sha
Affiliation1.Chongqing Med Univ, Sch Tradit Chinese Med, Chongqing 401331, Peoples R China
2.China Acad Tradit Chinese Med, Inst Chinese Mat Med, Beijing 100700, Peoples R China
3.Qianjiang Cent Hosp Chongqing, Chongqing 409099, Peoples R China
4.Chinese Acad Sci, Inst Psychol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Yibing Zhang,Zhao, Yong,Ran, Yongwang,et al. Alantolactone exhibits selective antitumor effects in HELA human cervical cancer cells by inhibiting cell migration and invasion, G2/M cell cycle arrest, mitochondrial mediated apoptosis and targeting Nf-kB signalling pathway[J]. JOURNAL OF BUON,2019,24(6):2310-2315.
APA Yibing Zhang,Zhao, Yong,Ran, Yongwang,Guo, Jianyou,Cui, Haifeng,&Liu, Sha.(2019).Alantolactone exhibits selective antitumor effects in HELA human cervical cancer cells by inhibiting cell migration and invasion, G2/M cell cycle arrest, mitochondrial mediated apoptosis and targeting Nf-kB signalling pathway.JOURNAL OF BUON,24(6),2310-2315.
MLA Yibing Zhang,et al."Alantolactone exhibits selective antitumor effects in HELA human cervical cancer cells by inhibiting cell migration and invasion, G2/M cell cycle arrest, mitochondrial mediated apoptosis and targeting Nf-kB signalling pathway".JOURNAL OF BUON 24.6(2019):2310-2315.
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