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Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation
Mohammed, Ibrahim1; Ijaz, Sahar1; Mokhtari, Tahmineh2,3; Gholaminejhad, Morteza1; Mahdavipour, Marzieh1; Jameie, Behnamedin4; Akbari, Mohammad1; Hassanzadeh, Gholamreza1
第一作者Mohammed, Ibrahim
通讯作者邮箱gholamreza hassanzadeh hassanzadeh@tums.ac.ir
心理所单位排序2
摘要

Spinal cord injury (SCI) is the destruction of spinal cord motor and sensory resulted from an attack on the spinal cord, which can cause significant physiological damage. The inflammasome is a multiprotein oligomer resulting in inflammation; the NLRP3 inflammasome composed of NLRP3, apoptosis-associated speck-like protein (ASC), procaspase-1, and cleavage of procaspase-1 into caspase-1 initiates the inflammatory response. Subventricular Zone (SVZ) is the origin of neural stem/progenitor cells (NS/PCs) in the adult brain. Extracellular vesicles (EVs) are tiny lipid membrane bilayer vesicles secreted by different types of cells playing an important role in cell-cell communications. The aim of this study was to investigate the effect of intrathecal transplantation of EVs on the NLRP3 inflammasome formation in SCI rats. Male wistar rats were divided into three groups as following: laminectotomy group, SCI group, and EVs group. EVs was isolated from SVZ, and characterized by western blot and DLS, and then injected into the SCI rats. Real-time PCR and western blot were carried out for gene expression and protein level of NLRP3, ASC, and Caspase-1. H&E and cresyl violet staining were performed for histological analyses, as well as BBB test for motor function. The results indicated high level in mRNA and protein level in SCI group in comparison with laminectomy (p < 0.001), and injection of EVs showed a significant reduction in the mRNA and protein levels in EVs group compared to SCI (p < 0.001). H&E and cresyl violet staining showed recovery in neural cells of spinal cord tissue in EVs group in comparison with SCI group. BBB test showed the promotion of motor function in EVs group compared to SCI in 14 days (p < 0.05). We concluded that the injection of EVs could recover the motor function in rats with SCI and rescue the neural cells of spinal cord tissue by suppressing the formation of the NLRP3 inflammasome complex.

关键词Sub-ventricular zone Extracellular vesicle Inflammasome Spinal cord injury Rat
2020-03-17
语种英语
DOI10.1007/s11011-020-00563-w
发表期刊METABOLIC BRAIN DISEASE
ISSN0885-7490
页码10
期刊论文类型article
收录类别SCI
资助项目Tehran University of Medical Sciences[97-01-103-37391]
出版者SPRINGER/PLENUM PUBLISHERS
WOS关键词STROMAL CELLS ; STEM-CELLS ; NEUROVASCULAR PLASTICITY ; CURRENT STATE ; BRAIN ; TRANSPLANTATION ; DELIVERY ; MICROPARTICLES ; ACTIVATION ; PLATFORM
WOS研究方向Endocrinology & Metabolism ; Neurosciences & Neurology
WOS类目Endocrinology & Metabolism ; Neurosciences
WOS记录号WOS:000520669000001
Q分类Q3
资助机构Tehran University of Medical Sciences
引用统计
被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/31491
专题中国科学院心理健康重点实验室
通讯作者Hassanzadeh, Gholamreza
作者单位1.Univ Tehran Med Sci, Sch Med, Dept Anat, Tehran, Iran
2.Inst Psychol, CAS Key Lab Mental Hlth, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Dept Psychol, Beijing, Peoples R China
4.Iran Univ Med Sci, Neurosci Res Ctr, Tehran, Iran
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GB/T 7714
Mohammed, Ibrahim,Ijaz, Sahar,Mokhtari, Tahmineh,et al. Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation[J]. METABOLIC BRAIN DISEASE,2020:10.
APA Mohammed, Ibrahim.,Ijaz, Sahar.,Mokhtari, Tahmineh.,Gholaminejhad, Morteza.,Mahdavipour, Marzieh.,...&Hassanzadeh, Gholamreza.(2020).Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation.METABOLIC BRAIN DISEASE,10.
MLA Mohammed, Ibrahim,et al."Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation".METABOLIC BRAIN DISEASE (2020):10.
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