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邻甲氧基桂皮醛对IL-1刺激脑微血管内皮细胞COX活性及PGE_2释放的影响
Alternative TitleEffect of 2-methoxycinnamaldehyde on activity of COX and PGE_2 release in cerebral microvascular endothelial cells stimulated by IL-1
郭建友; 杨元宵; 赵保胜; 刘洪斌; 李兰芳; 马悦颖; 郭淑英; 霍海如; 姜廷良
2006
Source Publication中国中药杂志
ISSN1001-5302
Volume31Issue:13Pages:1087-1090
Abstract目的:观察桂枝汤有效部位A分离的邻甲氧基桂皮醛对发热相关的环氧酶(COX)和前列腺素E2(PGE2)的影响。方法:建立大鼠脑微血管内皮细胞(rCMEC)培养,进行Ⅷ因子相关抗原鉴定。待细胞生长至融合状态后加入不同含量的邻甲氧基桂皮醛(12.5,25,50,100,200μg.mL-1)孵育3 h,之后以30 ng.mL-1的IL-1刺激12 h。ELISA方法检测细胞培养液中PGE2的含量及细胞内COX-1和COX-2的活性。结果:Ⅷ因子抗体免疫组化染色可见90%以上的培养细胞呈阳性,确认为rCMEC。暴露于30 ng.mL-1IL-1后,rCMEC内COX-2活性及释放的PGE2量显著增加,COX-1活性变化无统计学差异。加入不同含量的邻甲氧基桂皮醛后,随含量增加可下调COX-1,COX-2活性及PGE2量,且呈剂量依赖关系; 至含量为200μg.mL-1时,COX-2活性及释放的PGE2与IL-1单独作用组相比均有显著性差异,COX-1活性虽有所降低,但无统计学上的显著差异。结论:邻甲氧基桂皮醛能下调IL-1刺激rCMEC释放升高的PGE2,作用机制可能与抑制COX-2活性有关。
Keyword邻甲氧基桂皮醛 脑微血管内皮细胞 白介素1 环氧酶 前列腺素e2
Subject Area生理心理学/生物心理学 ; 医学心理学
Indexed By中文核心期刊要目总览 ASPT来源刊 CJFD收录刊
Language中文
Funding Project国家自然科学基金重大项目(90209006)
NoteObjective: To observe the effect of 2-methoxycinnamaldehyde(isolated from fraction A of Guizhi Tang) on activity of COX and PGE_(2) release in rat cerebral microvascular endothelial cells(rCMEC) stimulated by IL-1.Method: rCMEC were cultured,and identified by immunohistochemistry for von Willebrand factor(Ⅷ factor,a marker for all endothelial cells) in cytoplasm of the cells.Different concentrations of 2-methoxycinnamaldehyde were added respectively and incubated for 3 hours,then stimulated for another 12 h...
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/3150
Collection中国科学院心理研究所回溯数据库(1956-2010)
Corresponding Author霍海如
Recommended Citation
GB/T 7714
郭建友,杨元宵,赵保胜,等. 邻甲氧基桂皮醛对IL-1刺激脑微血管内皮细胞COX活性及PGE_2释放的影响[J]. 中国中药杂志,2006,31(13):1087-1090.
APA 郭建友.,杨元宵.,赵保胜.,刘洪斌.,李兰芳.,...&姜廷良.(2006).邻甲氧基桂皮醛对IL-1刺激脑微血管内皮细胞COX活性及PGE_2释放的影响.中国中药杂志,31(13),1087-1090.
MLA 郭建友,et al."邻甲氧基桂皮醛对IL-1刺激脑微血管内皮细胞COX活性及PGE_2释放的影响".中国中药杂志 31.13(2006):1087-1090.
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