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Candidate l-methionine target piRNA regulatory networks analysis response to cocaine-conditioned place preference in mice
Zhang, Kunlin1; Ji, Guanyu3; Zhao, Mei1,2; Wang, Yan1,2
First AuthorZhang, Kunlin
Correspondent Emailwyan@psych.ac.cn (wang, yan)
Contribution Rank1
Abstract

Background Methionine has been proven to inhibit addictive behaviors of cocaine dependence. However, the mechanism of methionine response to cocaine CPP is unknown. Recent evidence highlights piRNAs to regulate genes via a miRNA-like mechanism. Here, next-generation sequencing is used to study mechanism on methionine response to drug-induced behaviors though piRNA. Methods l-methionine treatment cocaine CPP animal model was used to do non-coding RNA sequencing. There were four groups to sequence: saline+saline (SS), MET+saline (MS), MET+cocaine (MC), and cocaine+saline. Combining mRNA sequencing data, the network and regulation of piRNA were analyzed with their corresponding mRNA and miRNA. Results Analysis of the piRNAome reveals that piRNAs inversely regulated their target mRNA genes. KEGG analysis of DE-piRNA target mRNA genes were enriched in Morphine addiction, GABAergic synapse and Cholinergic synapse pathway. Furthermore, four significantly differential expressed genes Cacna2d3, Epha6, Nedd4l, and Vav2 were identified and regulated by piRNAs in the process of l-methionine inhibits cocaine CPP. Thereinto, Vav2 was regulated by multiple DE piRNAs by sharing the common sequence: GTCTCTCCAGCCACCTT. Meanwhile, it was found that piRNA positively regulates miRNA and three genes Bcl3, Il20ra, and Insrr were identified and regulated by piRNA through miRNA. Conclusion The results showed that piRNA negatively regulated target mRNA genes and positively regulated target miRNA genes. Genes located in substance dependence, signal transduction and also nervous functions pathways were identified. When taken together, these data may explain the roles of l-methionine in counteracting the effects of cocaine CPP via piRNAs.

Keywordcocaine CPP gene expression high-throughput sequencing piRNA
2021-07-01
DOI10.1002/brb3.2272
Source PublicationBRAIN AND BEHAVIOR
ISSN2162-3279
Pages10
Subtype实证研究
Indexed BySCI
Funding ProjectNational Natural Science Foundation of China[61401459] ; National Natural Science Foundation of China[91132728] ; National Natural Science Foundation of China[31741062] ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences
PublisherWILEY
WOS KeywordPIWI-INTERACTING RNAS ; DNA METHYLATION ; NONCODING RNA ; IDENTIFICATION ; EXPRESSION ; PROTEINS ; GENES
WOS Research AreaBehavioral Sciences ; Neurosciences & Neurology
WOS SubjectBehavioral Sciences ; Neurosciences
WOS IDWOS:000668554500001
WoS QuartileQ3
Citation statistics
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/39807
Collection中国科学院心理健康重点实验室
Corresponding AuthorZhao, Mei; Wang, Yan
Affiliation1.CAS Key Lab Mental Hlth, Inst Psychol, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Dept Psychol, Beijing, Peoples R China
3.ShenZhen Gendo Hlth Technol Co Ltd, Shenzhen, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Kunlin,Ji, Guanyu,Zhao, Mei,et al. Candidate l-methionine target piRNA regulatory networks analysis response to cocaine-conditioned place preference in mice[J]. BRAIN AND BEHAVIOR,2021:10.
APA Zhang, Kunlin,Ji, Guanyu,Zhao, Mei,&Wang, Yan.(2021).Candidate l-methionine target piRNA regulatory networks analysis response to cocaine-conditioned place preference in mice.BRAIN AND BEHAVIOR,10.
MLA Zhang, Kunlin,et al."Candidate l-methionine target piRNA regulatory networks analysis response to cocaine-conditioned place preference in mice".BRAIN AND BEHAVIOR (2021):10.
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