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Acupuncture inhibits NLRP3 inflammasome activation in the prefrontal cortex of a chronic stress rat model of depression
Li, Xiaoyan1; Wang, Hongmei1; Li, Chen1; Wu, Jihong1; Lu, Jun1; Guo, Jian-You2; Tu, Ya1
First AuthorLi, Xiaoyan
Correspondent Emaillumed@126.com (jun lu) ; guojy@psych.ac.cn (jian-you guo)
Contribution Rank2
Abstract

The Nod-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome-driven inflammatory response plays a key role in the pathophysiology of depression. Mounting evidence suggests that acupuncture is an effective treatment for depression. In this study, we investigated the effects of acupuncture treatment at the acupoints Baihui (GV20) and Yintang (GV29) on NLRP3 inflammasome in the prefrontal cortex (PFC) of an animal model of depression. Rats that underwent chronic unpredictable mild stress (CUMS) for 6 weeks showed depressive-like behaviors, which were confirmed by sucrose preference and locomotor activity in the open field test. The protein levels of NLRP3, apoptotic speck-containing protein with a card (ASC), and cysteinyl aspartate specific proteinase-1 (Caspase-1) in the PFC were detected by Western blot analysis. The mRNA and protein expressions of interleukin-1 beta (IL-1 beta) and interleukin-18 (IL-18) in the PFC were measured by the real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Our results showed that the depressive-like behaviors in stressed rats were reversed by acupuncture treatment. Compared with control rats, the protein expression of NLRP3, ASC, and Caspase-1 and the mRNA and protein expressions of IL-1 beta and IL-18 in the PFC were markedly increased in CUMS rats. Acupuncture treatment significantly decreased the levels of NLRP3 inflammasome components and inflammatory cytokines in the PFC. Acupuncture treatment also reduced the number of TUNEL-positive cells in the PFC. These results suggested that acupuncture has antidepressant-like effects, and its mechanism appears to be involved in the inhibition of NLRP3 inflammasome activation and apoptosis in the PFC.

Keywordacupuncture chronic unpredictable stress depression inflammation NLRP3 inflammasome
2021-11-01
DOI10.1002/ar.24778
Source PublicationANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
ISSN1932-8486
Volume304Issue:11Pages:2470-2479
Subtype实证研究
Indexed BySCI
Funding ProjectNational Natural Science Foundation of China[81774418]
PublisherWILEY
WOS KeywordANTIDEPRESSANTS ; BEHAVIOR
WOS Research AreaAnatomy & Morphology
WOS SubjectAnatomy & Morphology
WOS IDWOS:000706083000001
WoS QuartileQ3
Funding OrganizationNational Natural Science Foundation of China
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Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/40665
Collection中国科学院心理健康重点实验室
Corresponding AuthorLu, Jun; Guo, Jian-You
Affiliation1.Beijing Univ Chinese Med, Coll Acupuncture Moxibust & Tuina, Beijing 100029, Peoples R China
2.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
Corresponding Author AffilicationKey Laboratory of Mental Health, CAS
Recommended Citation
GB/T 7714
Li, Xiaoyan,Wang, Hongmei,Li, Chen,et al. Acupuncture inhibits NLRP3 inflammasome activation in the prefrontal cortex of a chronic stress rat model of depression[J]. ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY,2021,304(11):2470-2479.
APA Li, Xiaoyan.,Wang, Hongmei.,Li, Chen.,Wu, Jihong.,Lu, Jun.,...&Tu, Ya.(2021).Acupuncture inhibits NLRP3 inflammasome activation in the prefrontal cortex of a chronic stress rat model of depression.ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY,304(11),2470-2479.
MLA Li, Xiaoyan,et al."Acupuncture inhibits NLRP3 inflammasome activation in the prefrontal cortex of a chronic stress rat model of depression".ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY 304.11(2021):2470-2479.
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