Gnas Promoter Hypermethylation in the Basolateral Amygdala Regulates Reconsolidation of Morphine Reward Memory in Rats

doi:10.3390/genes13030553

	Gnas Promoter Hypermethylation in the Basolateral Amygdala Regulates Reconsolidation of Morphine Reward Memory in Rats
	Liu, Peng 1,2; Liang, Jialong 3; Jiang, Fengze 1,2; Cai, Wanshi 3; Shen, Fang1,2 ; Liang, Jing1,2 ; Zhang, Jianjun1,2 ; Sun, Zhongsheng3,4 ; Sui, Nan1,2
通讯作者	Zhang, Jianjun(zhangjj@psych.ac.cn) ; Sun, Zhongsheng(sunzs@mail.biols.ac.cn)
摘要	Impairing reconsolidation may disrupt drug memories to prevent relapse, meanwhile long-term transcription regulations in the brain regions contribute to the occurrence of emotional memories. The basolateral amygdala (BLA) is involved in the drug-cue association, while the nucleus accumbens (NAc) responds to the drug reward. Here, we assessed whether DNA methyltransferases (Dnmts) in these two brain regions function identically in the reconsolidation of morphine reward memory. We show that Dnmts inhibition in the BLA but not in the NAc after memory retrieval impaired reconsolidation of a morphine reward memory. Moreover, the mRNA levels of Dnmt3a and Dnmt3b, rather than Dnmt1, in the BLA were continuously upregulated after retrieval. We further identified the differentially methylated regions (DMRs) in genes in the BLA after retrieval, and focused on the DMRs located in gene promoter regions. Among them were three genes (Gnas, Sox10, and Pik3r1) involved in memory modulation. Furthermore, Gnas promoter hypermethylation was confirmed to be inversely correlated with the downregulation of Gnas mRNA levels. The findings indicate that the specific transcription regulation mechanism in the BLA and NAc on reconsolidation of opiate-associated memories can be dissociable, and DNA hypermethylation of Gnas in the BLA is necessary for the reconsolidation of morphine reward memories.
关键词	DNA methylation DNA methyltransferases morphine basolateral amygdala reconsolidation memory
	2022-03-01
语种	英语
DOI	10.3390/genes13030553
发表期刊	GENES
卷号	13 期号:3 页码:16
收录类别	SCI
资助项目	Chinese National Programs for Brain Science and Brain-like Intelligence Technology[2021ZD0202104] ; National Key Research and Development Program of China[2016YFC0900400] ; National Natural Science Foundation of China[31571301] ; National Natural Science Foundation of China[31400880] ; National Natural Science Foundation of China[31871111] ; Youth Innovation Promotion Association CAS[2020088] ; Key Laboratory of Mental Health, Institute of Psychology, CAS
出版者	MDPI
WOS关键词	INDUCED PLACE PREFERENCE ; POST-RETRIEVAL EXTINCTION ; DNA METHYLATION ; NUCLEUS-ACCUMBENS ; PROTEIN-SYNTHESIS ; ACTIVATION ; RECEPTORS ; KINASE ; GENE ; NMDA
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000775235400001
资助机构	Chinese National Programs for Brain Science and Brain-like Intelligence Technology ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Key Laboratory of Mental Health, Institute of Psychology, CAS
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/42361
专题	中国科学院心理健康重点实验室
通讯作者	Zhang, Jianjun; Sun, Zhongsheng
作者单位	1.Inst Psychol, CAS Key Lab Mental Hlth, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Dept Psychol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China 4.Wenzhou Med Univ, Inst Genom Med, Wenzhou 325000, Peoples R China
第一作者单位	中国科学院心理健康重点实验室
通讯作者单位	中国科学院心理健康重点实验室
推荐引用方式 GB/T 7714	Liu, Peng,Liang, Jialong,Jiang, Fengze,et al. Gnas Promoter Hypermethylation in the Basolateral Amygdala Regulates Reconsolidation of Morphine Reward Memory in Rats[J]. GENES,2022,13(3):16.
APA	Liu, Peng.,Liang, Jialong.,Jiang, Fengze.,Cai, Wanshi.,Shen, Fang.,...&Sui, Nan.(2022).Gnas Promoter Hypermethylation in the Basolateral Amygdala Regulates Reconsolidation of Morphine Reward Memory in Rats.GENES,13(3),16.
MLA	Liu, Peng,et al."Gnas Promoter Hypermethylation in the Basolateral Amygdala Regulates Reconsolidation of Morphine Reward Memory in Rats".GENES 13.3(2022):16.