Dose Optimization of Anxiolytic Compounds Group in Valeriana jatamansi Jones and Mechanism Exploration by Integrating Network Pharmacology and Metabolomics Analysis

doi:10.3390/brainsci12050589

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	Dose Optimization of Anxiolytic Compounds Group in Valeriana jatamansi Jones and Mechanism Exploration by Integrating Network Pharmacology and Metabolomics Analysis
	Zhao, Chengbowen 1,2; Wei, Xiaojia 1; Guo, Jianyou 3; Ding, Yongsheng 1; Luo, Jing 1; Yang, Xue 1; Li, Jiayuan 1; Wan, Guohui 1; Yu, Jiahe 1; Shi, Jinli 1
通讯作者	Shi, Jinli(shijl@bucm.edu.com)
摘要	Anxiety disorder impacts the quality of life of the patients. The 95% ethanol extract of rhizomes and roots of Valeriana jatamansi Jones (Zhi zhu xiang, ZZX) has previously been shown to be effective for the treatment of anxiety disorder. In this study, the dose ratio of each component of the anxiolytic compounds group (ACG) in a 95% ethanol extract of ZZX was optimized by a uniform design experiment and mathematical modeling. The anxiolytic effect of ACG was verified by behavioral experiments and biochemical index measurement. Network pharmacology was used to determine potential action targets, as well as predict biological processes and signaling pathways, which were then verified by molecular docking analysis. Metabolomics was then used to screen and analyze metabolites in the rat hippocampus before and after the administration of ZZX-ACG. Finally, the results of metabolomics and network pharmacology were integrated to clarify the anti-anxiety mechanism of the ACG. The optimal dose ratio of ACG in 95% ethanol extract of ZZX was obtained, and our results suggest that ACG may regulate ALB, AKT1, PTGS2, CYP3A4, ESR1, CASP3, CYP2B6, EGFR, SRC, MMP9, IGF1, and MAPK8, as well as the prolactin signaling pathway, estrogen signaling pathway, and arachidonic acid metabolism pathway, thus affecting the brain neurotransmitters and HPA axis hormone levels to play an anxiolytic role, directly or indirectly.
关键词	Valeriana jatamansi Jones anxiety disorder network pharmacology metabolomics arachidonic acid metabolism
	2022-05-01
语种	英语
DOI	10.3390/brainsci12050589
发表期刊	BRAIN SCIENCES
卷号	12 期号:5 页码:21
收录类别	SCI
资助项目	National Natural Science Foundation of China[81673560] ; National Natural Science Foundation of China[82073971]
出版者	MDPI
WOS关键词	ANXIETY ; ESTROGEN ; RECEPTOR ; STRESS ; EXPRESSION ; PROLACTIN ; APOPTOSIS ; AMYGDALA ; EXTRACT ; GPR30
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
WOS记录号	WOS:000803450600001
资助机构	National Natural Science Foundation of China
引用统计
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/42747
通讯作者	Shi, Jinli
作者单位	1.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 102488, Peoples R China 2.Beijing Univ Chinese Med, Dongzhimen Hosp, Beijing 100007, Peoples R China 3.Chinese Acad Sci, Inst Psychol, CAS Key Lab Mental Hlth, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Chengbowen,Wei, Xiaojia,Guo, Jianyou,et al. Dose Optimization of Anxiolytic Compounds Group in Valeriana jatamansi Jones and Mechanism Exploration by Integrating Network Pharmacology and Metabolomics Analysis[J]. BRAIN SCIENCES,2022,12(5):21.
APA	Zhao, Chengbowen.,Wei, Xiaojia.,Guo, Jianyou.,Ding, Yongsheng.,Luo, Jing.,...&Shi, Jinli.(2022).Dose Optimization of Anxiolytic Compounds Group in Valeriana jatamansi Jones and Mechanism Exploration by Integrating Network Pharmacology and Metabolomics Analysis.BRAIN SCIENCES,12(5),21.
MLA	Zhao, Chengbowen,et al."Dose Optimization of Anxiolytic Compounds Group in Valeriana jatamansi Jones and Mechanism Exploration by Integrating Network Pharmacology and Metabolomics Analysis".BRAIN SCIENCES 12.5(2022):21.