Institutional Repository, Institute of Psychology, Chinese Academy of Sciences
中脑腹侧被盖区和伏隔核内食欲素Ⅰ型受体在大鼠觅药行为表达中的作用 | |
其他题名 | The Roles of OrexinⅠReceptor in Ventral Tegmental Area and Nucleus Accumbens in Drug-seeking Behavior Induced by Morphine |
白云静1,2; 黄砚北1,2; 魏楚光1,2; 彭永华1,2; 李勇辉1; 隋南1 | |
第一作者 | 白云静 |
通讯作者邮箱 | suin@psych.ac.cn (隋南) |
心理所单位排序 | 1 |
摘要 | 目的:探讨中脑腹侧被盖区(VTA)和伏隔核(NAc)内食欲素Ⅰ型受体在大鼠觅药行为表达中的作用。方法:以吗啡条件性位置偏爱(CPP)表达作为大鼠觅药行为模型,88只雄性Wistar大鼠随机进入VTA干预组和NAc干预组,偏爱测试前分别在VTA和NAc内注射食欲素Ⅰ型受体(OXR1)拮抗剂SB334867(VTA:1,5μg;NAc:1,3μg)或溶剂(DMSO),考察其对CPP表达的影响。采用单因素方差分析比较了各组间的偏爱值。结果:吗啡CPP表达前VTA注射5μgSB334867组动物的偏爱值(73.6±81.7)低于溶剂处理组(183.5±30.4);吗啡CPP表达前NAc注射SB334867组动物的偏爱值(229.6±72.9和260.6±53.9)与溶剂处理组(224±52.1)之间无显著差异。结论:食欲素通过激活VTA内食欲素Ⅰ型受体促进药物相关环境线索诱发的大鼠觅药行为表达,而NAc内的食欲素Ⅰ型受体不参与觅药行为表达。本研究为揭示食欲素参与觅药行为表达的神经机制提供了线索。 |
其他摘要 | Objective: To investigate the roles of OXR1 in the ventral tegmental area (VTA) and nucleus accumbens ( NAc) in drug-seeking behavior induced by morphine. Methods: Drug-seeking behavior in rats was measured with the model of expression of morphine conditioned place preference ( CPP ). Eighty-eight male Wistar rats were randomly incorporated into the VTA-treated groups and NAc-treated groups. The OXR1 selective antagonist SB334867 (VTA: 1, 5μg; NAc: 1, 3μg) or vehicle (Dmethyl Sulfoxide) were microinjected into the VTA and NAc respectively before CPP expression tests. The one-way ANOVA was used to compare the preference scores among groups. Results: The preference score was significantly reduced in animals microinjected with 5μg SB334867 compared with that in animals microinjected with vehicle into the VTA before CPP expression test [ (73.6 ± 81.7) vs. ( 183.5 ±30. 4) , P 〈 0. 05 ] ; There was no significant difference between the preference score in animals microinjected with SB334867 (229. 6 ±72. 9 and 260. 6 ± 53.9) and that in animals mieroinjected with vehicle (224 ±52.1 ) into the NAe before CPP expression test. Conclusion: The activation of OXR1 in the VTA plays an important role in drug-seeking behavior elicited by morphine-associated environmental cues, while OXR1 in the NAc is not involved in this behavior. The present study suggests some neural mechanisms underlying the roles of orexins in drug-seeking behavior. |
关键词 | 药物成瘾 条件性位置偏爱 食欲素 觅药行为 中脑腹侧被盖区 伏隔核 |
学科领域 | 生理心理学/生物心理学 |
2009 | |
语种 | 中文 |
发表期刊 | 中国心理卫生杂志 |
ISSN | 1000-6729 |
卷号 | 23期号:6页码:441-445 |
期刊论文类型 | 实证研究 |
收录类别 | CSCD ; CSSCI ; 中文核心期刊要目总览 |
项目简介 | 国家重点基础研究发展计划(973计划)(2003CB515404 2009CB522002) 国家自然科学基金(30770719 30600184) 中国科学院知识创新工程重要方向项目(KSCX1-TW-R-68) |
CSCD记录号 | CSCD:3542862 |
附注 | Objective:To investigate the roles of OXR1 in the ventral tegmental area(VTA)and nucleus accumbens(NAc)in drug-seeking behavior induced by morphine.Methods:Drug-seeking behavior in rats was measured with the model of expression of morphine conditioned place preference(CPP).Eighty-eight male Wistar rats were randomly incorporated into the VTA-treated groups and NAc-treated groups.The OXR1 selective antagonist SB334867(VTA:1,5μg; NAc:1,3μg)or vehicle(Dmethyl Sulfoxide)were microinjected into the VTA and NAc respectively before CPP expression tests.The one-way ANOVA was used to compare the preference scores among groups.Results:The preference score was significantly reduced in animals microinjected with 5μg SB334867 compared with that in animals microinjected with vehicle into the VTA before CPP expression test [(73.6±81.7)vs.(183.5±30.4),P<0.05]; There was no significant difference between the preference score in animals microinjected with SB334867(229.6±72.9 and 260.6±53.9)and that in animals microinjected with vehicle(224±52.1)into the NAc before CPP expression test.Conclusion:The activation of OXR1 in the VTA plays an important role in drug-seeking behavior elicited by morphine-associated environmental cues,while OXR1 in the NAc is not involved in this behavior.The present study suggests some neural mechanisms underlying the roles of orexins in drug-seeking behavior. |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.psych.ac.cn/handle/311026/4340 |
专题 | 中国科学院心理研究所回溯数据库(1956-2010) |
通讯作者 | 隋南 |
作者单位 | 1.中国科学院心理研究所 , 心理健康重点实验室 , 北京 100101 2.中国科学院研究生院 , 北京 100087 |
第一作者单位 | 中国科学院心理研究所 |
通讯作者单位 | 中国科学院心理研究所 |
推荐引用方式 GB/T 7714 | 白云静,黄砚北,魏楚光,等. 中脑腹侧被盖区和伏隔核内食欲素Ⅰ型受体在大鼠觅药行为表达中的作用[J]. 中国心理卫生杂志,2009,23(6):441-445. |
APA | 白云静,黄砚北,魏楚光,彭永华,李勇辉,&隋南.(2009).中脑腹侧被盖区和伏隔核内食欲素Ⅰ型受体在大鼠觅药行为表达中的作用.中国心理卫生杂志,23(6),441-445. |
MLA | 白云静,et al."中脑腹侧被盖区和伏隔核内食欲素Ⅰ型受体在大鼠觅药行为表达中的作用".中国心理卫生杂志 23.6(2009):441-445. |
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