Institutional Repository of Key Laboratory of Mental Health, CAS
Effect of early embryonic exposure to morphine on defects in the GABAergic system of day-old chicks | |
Wang, Dongmei1,2; Jiang, Juan1,2; Shang, Wen1,2; Zhang, Jianjun1,2; Jiang, Xiao1,2; Shen, Fang1,2; Liang, Jing1,2; Li, Yonghui1,2; Li, Ming3; Wang, Mengya1,4; Sui, Nan1,2,5 | |
第一作者 | Dongmei Wang |
通讯作者邮箱 | wangmy@wnmc.edu.cn (m. wang) ; suin@psych.ac.cn (n. sui) |
心理所单位排序 | 1 |
摘要 | Embryonic morphine exposure (EME) leads to abnormal brain development and behavior in the offspring, and the functional alteration of gamma-aminobutyric acid (GABA) system is considered to be one of the important mechanisms. To mimic the problem of susceptibility of human gestational drug abuse on addictive drugs in offspring, we administered morphine exposure on days 5-8 and 13-16 of chicken embryo development and examined the functions of GABA neurons and their receptors in postnatal chicks by neuroelectrophysiology, immunohistochemistry and behavioral methods. We found that morphine exposure during embryonic stages 5-8 (MorphineE5-8) significantly reduced the incidence of spontaneous inhibitory postsynaptic potentiation (IPSP) and the induction of evoked IPSP and the mean amplitude of GABAA agonist muscimol-induced response in the intermediate medial interstitial (IMM) region, compared to naive controls or saline-exposed chicks. The results of immunocytochemistry further suggest that MorphineE5-8 decreased the synaptic density of GAD-expressing sites in the IMM, while increased the expression of the GABAA receptor subtype gamma 2 isoform. Behavioral results found that Morphine5-8 treatment de-inhibited morphine-induced psychomotor responses in postnatal chicks. Morphine exposure at embryonic stages 13-16 (MorphineE13-16) showed no significant changes in the above indicators compared to the saline group. Evidence suggests that early embryonic morphine exposure leads to defects in GABAergic function in the IMM, which in turn alters the responsiveness of postnatal chicks to addictive drugs. These results will help to understand the GABA mechanisms by which embryonic addictive drug exposure contributes to offspring susceptibility to addiction. |
关键词 | Embryonic morphine exposure gamma-Aminobutyric acid Neuroelectrophysiology Intermediate medial mesopallium (IMM) Chicks Addictive susceptibility |
2023-03-08 | |
语种 | 英语 |
DOI | 10.1016/j.pnpbp.2022.110657 |
发表期刊 | PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY |
ISSN | 0278-5846 |
卷号 | 121页码:9 |
期刊论文类型 | 实证研究 |
收录类别 | SSCI |
资助项目 | National Natural Science Foundation[31300848] ; Key Laboratory of Mental Health, Institute of Psychology, CAS |
出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
WOS关键词 | GAMMA-AMINOBUTYRIC-ACID ; INTERMEDIATE MEDIAL MESOPALLIUM ; VENTRAL TEGMENTAL AREA ; PRENATAL EXPOSURE ; SEIZURE SUSCEPTIBILITY ; REWARDING PROPERTIES ; GABA(B) RECEPTORS ; OPIOID RECEPTORS ; PLACE PREFERENCE ; PRESYNAPTIC MU |
WOS研究方向 | Neurosciences & Neurology ; Pharmacology & Pharmacy ; Psychiatry |
WOS类目 | Clinical Neurology ; Neurosciences ; Pharmacology & Pharmacy ; Psychiatry |
WOS记录号 | WOS:000882071600001 |
WOS分区 | Q1 |
资助机构 | National Natural Science Foundation ; Key Laboratory of Mental Health, Institute of Psychology, CAS |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.psych.ac.cn/handle/311026/43944 |
专题 | 中国科学院心理健康重点实验室 |
通讯作者 | Wang, Mengya; Sui, Nan |
作者单位 | 1.Inst Psychol, CAS Key Lab Mental Hlth, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Dept Psychol, Beijing, Peoples R China 3.Univ Nebraska Lincoln, Dept Psychol, Lincoln, NE USA 4.Wannan Med Coll, Cell Electrophysiol Lab, Wuhu, Peoples R China 5.Chinese Acad Sci, Inst Psychol, 16 Lincui Rd, Beijing 100101, Peoples R China |
第一作者单位 | 中国科学院心理健康重点实验室 |
通讯作者单位 | 中国科学院心理健康重点实验室; 中国科学院心理研究所 |
推荐引用方式 GB/T 7714 | Wang, Dongmei,Jiang, Juan,Shang, Wen,et al. Effect of early embryonic exposure to morphine on defects in the GABAergic system of day-old chicks[J]. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY,2023,121:9. |
APA | Wang, Dongmei.,Jiang, Juan.,Shang, Wen.,Zhang, Jianjun.,Jiang, Xiao.,...&Sui, Nan.(2023).Effect of early embryonic exposure to morphine on defects in the GABAergic system of day-old chicks.PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY,121,9. |
MLA | Wang, Dongmei,et al."Effect of early embryonic exposure to morphine on defects in the GABAergic system of day-old chicks".PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY 121(2023):9. |
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