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Distinctive roles of L-type calcium channels subtypes within the dorsal hippocampus in formation of morphine withdrawal-induced aversion in rats | |
Duan, Ying1,2; Jin, Lingtong1,2; Du, Wenjie1,2; Meng, Yiming1,2; Liang, Jing1,2; Zhang, Jianjun1,2; Sui, Nan1,2,3; Shen, Fang1,2,3 | |
第一作者 | Duan, Ying |
通讯作者邮箱 | shenf@psych.nc.cn (f. shen) |
摘要 | Although the negative effects coming along with opiate withdrawal are in part modulated by L-type calcium channels (LTCCs), the distinctive physiological properties and functions of LTCCs subtypes suggest differential roles of subtypes during withdrawal. The present study aimed to examine the contributions of LTCC subtypes, Cav1.2 and Cav1.3, within the dorsal hippocampus (DH) in naloxone-precipitated morphine withdrawal using the conditioned place aversion (CPA) paradigm. Firstly, we injected the non-specific LTCCs antagonist verapamil into the DH of morphine-dependent rats before conditioning an environment with naloxone-precipitated withdrawal. Our results showed that verapamil blocked the acquisition of CPA. Then, to explore the molecular mechanisms of LTCCs subtypes during withdrawal, we measured the protein expression of Cav1.2 and Cav1.3 in morphine-dependent rats under different conditions. In morphine-dependent rats, conditioning with withdrawal increased Cav1.2 expression in the membrane, while only acute naloxone injection increased the membrane expression of Cav1.3. To further determine the causal roles of LTCCs subtypes in the withdrawal process, we used Cav1.2 siRNA or Cav1.3 shRNA to knock down the expression of subtypes and detected the effects on CPA and somatic withdrawal signs in morphine-dependent rats. Cav1.2 siRNA, but not Cav1.3 shRNA, inhibited the acquirement of CPA and relieved somatic withdrawal symptoms. Together, our findings reveal that Cav1.2, but not Cav1.3 plays an important role in mediating morphine withdrawal, suggesting this subtype may serve as a potential therapeutic target for the treatment of negative effects in opiate dependence. |
关键词 | Opiate withdrawal conditioned place aversion (CPA) dorsal hippocampus (DH) LTCCs subtypes RNA interference |
2023-12-20 | |
语种 | 英语 |
DOI | 10.1016/j.pnpbp.2023.110818 |
发表期刊 | PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY |
ISSN | 0278-5846 |
卷号 | 127页码:8 |
期刊论文类型 | 实证研究 |
收录类别 | SCI |
资助项目 | National Natural Science Foundation of China[31970995] ; National Natural Science Foundation of China[GJHZ202129] ; CAS-VPST Silk Road Science Fund ; CAS Key Laboratory of Mental Health, Institute of Psychology ; [32271137] |
出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
WOS关键词 | CONDITIONED PLACE AVERSION ; LONG-TERM POTENTIATION ; SYNAPTIC PLASTICITY ; NMDA RECEPTORS ; OPIATE WITHDRAWAL ; NUCLEUS-ACCUMBENS ; CA2+ CHANNELS ; NALOXONE ; CA(V)1.2 ; DEPENDENCE |
WOS研究方向 | Neurosciences & Neurology ; Pharmacology & Pharmacy ; Psychiatry |
WOS类目 | Clinical Neurology ; Neurosciences ; Pharmacology & Pharmacy ; Psychiatry |
WOS记录号 | WOS:001058480400001 |
WOS分区 | Q1 |
资助机构 | National Natural Science Foundation of China ; CAS-VPST Silk Road Science Fund ; CAS Key Laboratory of Mental Health, Institute of Psychology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.psych.ac.cn/handle/311026/45939 |
专题 | 中国科学院心理健康重点实验室 |
通讯作者 | Sui, Nan; Shen, Fang |
作者单位 | 1.Chinese Acad Sci, Inst Psychol, CAS Key Lab Mental Hlth, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Dept Psychol, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, 16 Lin Cui Rd, Beijing 100101, Peoples R China |
第一作者单位 | 中国科学院心理健康重点实验室 |
通讯作者单位 | 中国科学院心理健康重点实验室 |
推荐引用方式 GB/T 7714 | Duan, Ying,Jin, Lingtong,Du, Wenjie,et al. Distinctive roles of L-type calcium channels subtypes within the dorsal hippocampus in formation of morphine withdrawal-induced aversion in rats[J]. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY,2023,127:8. |
APA | Duan, Ying.,Jin, Lingtong.,Du, Wenjie.,Meng, Yiming.,Liang, Jing.,...&Shen, Fang.(2023).Distinctive roles of L-type calcium channels subtypes within the dorsal hippocampus in formation of morphine withdrawal-induced aversion in rats.PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY,127,8. |
MLA | Duan, Ying,et al."Distinctive roles of L-type calcium channels subtypes within the dorsal hippocampus in formation of morphine withdrawal-induced aversion in rats".PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY 127(2023):8. |
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