Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression
Wang, Kang2; Wang, Yanling2; Gao, Lixin2; Li, Xinmin2; Li, Mingming2; Guo, Jianyou1; J. Y. Guo
2008-08-01
发表期刊BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN0918-6158
文章类型Article
卷号31期号:8页码:1541-1546
摘要Intravitreal injection of corticosteroid has been used to treat diabetic macular edema, however, the exact mechanism remains unknown. In the present experiment, four weeks after streptozotocin administration, intravitreal injection of dexamethasone (50 mu g/10 mu l) was performed. After 2 d injection, we investigated the effect of dexamethasone on leukocyte accumulation, vascular permeability and the expression of vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1) in streptozotocin-diabetic rats. Accumulated leukocytes were counted in vivo by acridine orange leukocyte fluorography, the retinal vascular permeability was measured by the Evans blue assay. The mRNA and protein level of VEGF and ICAM-1 were analyzed with real-time quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) respectively. Dexamethasone downregulated VEGF and ICAM-1 expression in diabetic rats which correlated with its effect on leukocytes accumulation and retinal vascular permeability. The present data revealed that dexamethasone may inhibit retinal accumulation and leukostasis accumulation and vascular permeability through its blockage on VEGF and ICAM-1 expression.; Intravitreal injection of corticosteroid has been used to treat diabetic macular edema, however, the exact mechanism remains unknown. In the present experiment, four weeks after streptozotocin administration, intravitreal injection of dexamethasone (50 mu g/10 mu l) was performed. After 2 d injection, we investigated the effect of dexamethasone on leukocyte accumulation, vascular permeability and the expression of vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1) in streptozotocin-diabetic rats. Accumulated leukocytes were counted in vivo by acridine orange leukocyte fluorography, the retinal vascular permeability was measured by the Evans blue assay. The mRNA and protein level of VEGF and ICAM-1 were analyzed with real-time quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) respectively. Dexamethasone downregulated VEGF and ICAM-1 expression in diabetic rats which correlated with its effect on leukocytes accumulation and retinal vascular permeability. The present data revealed that dexamethasone may inhibit retinal accumulation and leukostasis accumulation and vascular permeability through its blockage on VEGF and ICAM-1 expression.
关键词dexamethasone vascular endothelial growth factor intercellular adhesion molecule-1 diabetes
学科领域生理心理学/生物心理学
收录类别SCI
语种英语
WOS记录号WOS:000259174000013
引用统计
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/5311
专题中国科学院心理研究所回溯数据库(1956-2010)
通讯作者J. Y. Guo
作者单位1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Healthy, Beijing 100101, Peoples R China
2.Beijing Capital Med Univ, Affiliated Beijing Friendship Hosp, Dept Ophthalmol, Beijing 100050, Peoples R China
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Wang, Kang,Wang, Yanling,Gao, Lixin,et al. Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2008,31(8):1541-1546.
APA Wang, Kang.,Wang, Yanling.,Gao, Lixin.,Li, Xinmin.,Li, Mingming.,...&J. Y. Guo.(2008).Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression.BIOLOGICAL & PHARMACEUTICAL BULLETIN,31(8),1541-1546.
MLA Wang, Kang,et al."Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression".BIOLOGICAL & PHARMACEUTICAL BULLETIN 31.8(2008):1541-1546.
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