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Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression
Wang, Kang2; Wang, Yanling2; Gao, Lixin2; Li, Xinmin2; Li, Mingming2; Guo, Jianyou1; J. Y. Guo
2008-08-01
Source PublicationBIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN0918-6158
SubtypeArticle
Volume31Issue:8Pages:1541-1546
AbstractIntravitreal injection of corticosteroid has been used to treat diabetic macular edema, however, the exact mechanism remains unknown. In the present experiment, four weeks after streptozotocin administration, intravitreal injection of dexamethasone (50 mu g/10 mu l) was performed. After 2 d injection, we investigated the effect of dexamethasone on leukocyte accumulation, vascular permeability and the expression of vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1) in streptozotocin-diabetic rats. Accumulated leukocytes were counted in vivo by acridine orange leukocyte fluorography, the retinal vascular permeability was measured by the Evans blue assay. The mRNA and protein level of VEGF and ICAM-1 were analyzed with real-time quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) respectively. Dexamethasone downregulated VEGF and ICAM-1 expression in diabetic rats which correlated with its effect on leukocytes accumulation and retinal vascular permeability. The present data revealed that dexamethasone may inhibit retinal accumulation and leukostasis accumulation and vascular permeability through its blockage on VEGF and ICAM-1 expression.; Intravitreal injection of corticosteroid has been used to treat diabetic macular edema, however, the exact mechanism remains unknown. In the present experiment, four weeks after streptozotocin administration, intravitreal injection of dexamethasone (50 mu g/10 mu l) was performed. After 2 d injection, we investigated the effect of dexamethasone on leukocyte accumulation, vascular permeability and the expression of vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1) in streptozotocin-diabetic rats. Accumulated leukocytes were counted in vivo by acridine orange leukocyte fluorography, the retinal vascular permeability was measured by the Evans blue assay. The mRNA and protein level of VEGF and ICAM-1 were analyzed with real-time quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) respectively. Dexamethasone downregulated VEGF and ICAM-1 expression in diabetic rats which correlated with its effect on leukocytes accumulation and retinal vascular permeability. The present data revealed that dexamethasone may inhibit retinal accumulation and leukostasis accumulation and vascular permeability through its blockage on VEGF and ICAM-1 expression.
Keyworddexamethasone vascular endothelial growth factor intercellular adhesion molecule-1 diabetes
Subject Area生理心理学/生物心理学
Indexed BySCI
Language英语
WOS IDWOS:000259174000013
Citation statistics
Cited Times:73[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/5311
Collection中国科学院心理研究所回溯数据库(1956-2010)
Corresponding AuthorJ. Y. Guo
Affiliation1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Healthy, Beijing 100101, Peoples R China
2.Beijing Capital Med Univ, Affiliated Beijing Friendship Hosp, Dept Ophthalmol, Beijing 100050, Peoples R China
Recommended Citation
GB/T 7714
Wang, Kang,Wang, Yanling,Gao, Lixin,et al. Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2008,31(8):1541-1546.
APA Wang, Kang.,Wang, Yanling.,Gao, Lixin.,Li, Xinmin.,Li, Mingming.,...&J. Y. Guo.(2008).Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression.BIOLOGICAL & PHARMACEUTICAL BULLETIN,31(8),1541-1546.
MLA Wang, Kang,et al."Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression".BIOLOGICAL & PHARMACEUTICAL BULLETIN 31.8(2008):1541-1546.
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