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Distribution and transcription activity of nucleolar DNA in higher plant cells
Tao, W; Xu, W; Valdivia, MM; Hao, S; Zhai, ZH; W. Tao
2001
Source PublicationCELL BIOLOGY INTERNATIONAL
ISSN1065-6995
SubtypeArticle
Volume25Issue:11Pages:1167-1171
AbstractBy using the NAMA-Ur DNA selective staining method, we have observed in situ the location of nucleolar DNA in onion cells and found it at the boundary between fibrillar centres (FC) and dense fibrillar component (DFC) in transcriptionally active nucleolus. We have also used anti-NOR serum, which is identified as the RNA Polymerase I transcription factor (UBF) antibody, to study its reactivity with higher plant cells and demonstrated this factor associated to the DFC but not present at the interior of FC. Finally, by employing anti-DNA/RNA hybrid antibodies, we labeled the transcriptionally active rRNA genes in active nucleolus and testified that at the boundary between FC and DFC. The results provide the evidence that the boundary between FC and DFC is the genuine transcription site of rRNA genes in nucleolus.; By using the NAMA-Ur DNA selective staining method, we have observed in situ the location of nucleolar DNA in onion cells and found it at the boundary between fibrillar centres (FC) and dense fibrillar component (DFC) in transcriptionally active nucleolus. We have also used anti-NOR serum, which is identified as the RNA Polymerase I transcription factor (UBF) antibody, to study its reactivity with higher plant cells and demonstrated this factor associated to the DFC but not present at the interior of FC. Finally, by employing anti-DNA/RNA hybrid antibodies, we labeled the transcriptionally active rRNA genes in active nucleolus and testified that at the boundary between FC and DFC. The results provide the evidence that the boundary between FC and DFC is the genuine transcription site of rRNA genes in nucleolus. (C) 2001 Academic Press.
Keywordnucleolus location of nucleolar DNA in situ transcription sites of rRNA genes RNA polymerase I transcription factor (UBF) Alllium cepa
Subject Area生物学
Indexed BySCI
Language英语
WOS IDWOS:000172524000011
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/5323
Collection中国科学院心理研究所回溯数据库(1956-2010)
Corresponding AuthorW. Tao
Affiliation1.Peking Univ, Coll Life Sci, Dept Cell Biol, Sch Life Sci, Beijing 100871, Peoples R China
2.NE Normal Univ, Inst Cytol & Genet, Changchun 130024, Jiling, Peoples R China
3.Univ Cadiz, Fac Sci, Dept Biochem & Mol Biol, Cadiz 11510, Spain
4.Acad Sinica, Inst Psychol, Dept Med Physiol & Psychol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Tao, W,Xu, W,Valdivia, MM,et al. Distribution and transcription activity of nucleolar DNA in higher plant cells[J]. CELL BIOLOGY INTERNATIONAL,2001,25(11):1167-1171.
APA Tao, W,Xu, W,Valdivia, MM,Hao, S,Zhai, ZH,&W. Tao.(2001).Distribution and transcription activity of nucleolar DNA in higher plant cells.CELL BIOLOGY INTERNATIONAL,25(11),1167-1171.
MLA Tao, W,et al."Distribution and transcription activity of nucleolar DNA in higher plant cells".CELL BIOLOGY INTERNATIONAL 25.11(2001):1167-1171.
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