Distribution and transcription activity of nucleolar DNA in higher plant cells
Tao, W; Xu, W; Valdivia, MM; Hao, S; Zhai, ZH; W. Tao
2001
发表期刊CELL BIOLOGY INTERNATIONAL
ISSN1065-6995
文章类型Article
卷号25期号:11页码:1167-1171
摘要By using the NAMA-Ur DNA selective staining method, we have observed in situ the location of nucleolar DNA in onion cells and found it at the boundary between fibrillar centres (FC) and dense fibrillar component (DFC) in transcriptionally active nucleolus. We have also used anti-NOR serum, which is identified as the RNA Polymerase I transcription factor (UBF) antibody, to study its reactivity with higher plant cells and demonstrated this factor associated to the DFC but not present at the interior of FC. Finally, by employing anti-DNA/RNA hybrid antibodies, we labeled the transcriptionally active rRNA genes in active nucleolus and testified that at the boundary between FC and DFC. The results provide the evidence that the boundary between FC and DFC is the genuine transcription site of rRNA genes in nucleolus.; By using the NAMA-Ur DNA selective staining method, we have observed in situ the location of nucleolar DNA in onion cells and found it at the boundary between fibrillar centres (FC) and dense fibrillar component (DFC) in transcriptionally active nucleolus. We have also used anti-NOR serum, which is identified as the RNA Polymerase I transcription factor (UBF) antibody, to study its reactivity with higher plant cells and demonstrated this factor associated to the DFC but not present at the interior of FC. Finally, by employing anti-DNA/RNA hybrid antibodies, we labeled the transcriptionally active rRNA genes in active nucleolus and testified that at the boundary between FC and DFC. The results provide the evidence that the boundary between FC and DFC is the genuine transcription site of rRNA genes in nucleolus. (C) 2001 Academic Press.
关键词nucleolus location of nucleolar DNA in situ transcription sites of rRNA genes RNA polymerase I transcription factor (UBF) Alllium cepa
学科领域生物学
收录类别SCI
语种英语
WOS记录号WOS:000172524000011
引用统计
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/5323
专题中国科学院心理研究所回溯数据库(1956-2010)
通讯作者W. Tao
作者单位1.Peking Univ, Coll Life Sci, Dept Cell Biol, Sch Life Sci, Beijing 100871, Peoples R China
2.NE Normal Univ, Inst Cytol & Genet, Changchun 130024, Jiling, Peoples R China
3.Univ Cadiz, Fac Sci, Dept Biochem & Mol Biol, Cadiz 11510, Spain
4.Acad Sinica, Inst Psychol, Dept Med Physiol & Psychol, Beijing 100101, Peoples R China
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Tao, W,Xu, W,Valdivia, MM,et al. Distribution and transcription activity of nucleolar DNA in higher plant cells[J]. CELL BIOLOGY INTERNATIONAL,2001,25(11):1167-1171.
APA Tao, W,Xu, W,Valdivia, MM,Hao, S,Zhai, ZH,&W. Tao.(2001).Distribution and transcription activity of nucleolar DNA in higher plant cells.CELL BIOLOGY INTERNATIONAL,25(11),1167-1171.
MLA Tao, W,et al."Distribution and transcription activity of nucleolar DNA in higher plant cells".CELL BIOLOGY INTERNATIONAL 25.11(2001):1167-1171.
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