Distribution and transcription activity of nucleolar DNA in higher plant cells
Tao, W; Xu, W; Valdivia, MM; Hao, S; Zhai, ZH; W. Tao
摘要By using the NAMA-Ur DNA selective staining method, we have observed in situ the location of nucleolar DNA in onion cells and found it at the boundary between fibrillar centres (FC) and dense fibrillar component (DFC) in transcriptionally active nucleolus. We have also used anti-NOR serum, which is identified as the RNA Polymerase I transcription factor (UBF) antibody, to study its reactivity with higher plant cells and demonstrated this factor associated to the DFC but not present at the interior of FC. Finally, by employing anti-DNA/RNA hybrid antibodies, we labeled the transcriptionally active rRNA genes in active nucleolus and testified that at the boundary between FC and DFC. The results provide the evidence that the boundary between FC and DFC is the genuine transcription site of rRNA genes in nucleolus.; By using the NAMA-Ur DNA selective staining method, we have observed in situ the location of nucleolar DNA in onion cells and found it at the boundary between fibrillar centres (FC) and dense fibrillar component (DFC) in transcriptionally active nucleolus. We have also used anti-NOR serum, which is identified as the RNA Polymerase I transcription factor (UBF) antibody, to study its reactivity with higher plant cells and demonstrated this factor associated to the DFC but not present at the interior of FC. Finally, by employing anti-DNA/RNA hybrid antibodies, we labeled the transcriptionally active rRNA genes in active nucleolus and testified that at the boundary between FC and DFC. The results provide the evidence that the boundary between FC and DFC is the genuine transcription site of rRNA genes in nucleolus. (C) 2001 Academic Press.
关键词nucleolus location of nucleolar DNA in situ transcription sites of rRNA genes RNA polymerase I transcription factor (UBF) Alllium cepa
学科领域生物学
2001
语种英语
发表期刊CELL BIOLOGY INTERNATIONAL
ISSN1065-6995
卷号25期号:11页码:1167-1171
期刊论文类型Article
收录类别SCI
WOS记录号WOS:000172524000011
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/5323
专题中国科学院心理研究所回溯数据库(1956-2010)
通讯作者W. Tao
作者单位1.Peking Univ, Coll Life Sci, Dept Cell Biol, Sch Life Sci, Beijing 100871, Peoples R China
2.NE Normal Univ, Inst Cytol & Genet, Changchun 130024, Jiling, Peoples R China
3.Univ Cadiz, Fac Sci, Dept Biochem & Mol Biol, Cadiz 11510, Spain
4.Acad Sinica, Inst Psychol, Dept Med Physiol & Psychol, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714
Tao, W,Xu, W,Valdivia, MM,et al. Distribution and transcription activity of nucleolar DNA in higher plant cells[J]. CELL BIOLOGY INTERNATIONAL,2001,25(11):1167-1171.
APA Tao, W,Xu, W,Valdivia, MM,Hao, S,Zhai, ZH,&W. Tao.(2001).Distribution and transcription activity of nucleolar DNA in higher plant cells.CELL BIOLOGY INTERNATIONAL,25(11),1167-1171.
MLA Tao, W,et al."Distribution and transcription activity of nucleolar DNA in higher plant cells".CELL BIOLOGY INTERNATIONAL 25.11(2001):1167-1171.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Tao-2001Distribution(526KB) 开放获取--浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Tao, W]的文章
[Xu, W]的文章
[Valdivia, MM]的文章
百度学术
百度学术中相似的文章
[Tao, W]的文章
[Xu, W]的文章
[Valdivia, MM]的文章
必应学术
必应学术中相似的文章
[Tao, W]的文章
[Xu, W]的文章
[Valdivia, MM]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Tao-2001Distribution and transcription.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。