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Proteomic analysis of rat prefrontal cortex in three phases of morphine-induced conditioned place preference
Yang, Liu; Sun, Zhong Sheng; Zhu, Yong-ping; Y. P. Zhu
2007
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
SubtypeArticle
Volume6Issue:6Pages:2239-2247
AbstractMorphological alterations of synapse are found after morphine administration, suggesting that regulation of synaptic plasticity may be one of the mechanisms of neuroadaptation in addiction. However, the molecular basis underlying the abnormal synapse morphological and physiological changes in the morphine-induced dependence, withdraw, and relapse is not well understood. As prefrontal cortex ( PFC) is one of the most important brain regions, which provides executive control over drug use and is severely impaired in many addicts, systematic analysis of the biochemical and molecular alteration of synaptic fraction of PFC in morphine-induced neuroadaptation is necessary. In this study, differential protein expression profiling of synaptic fraction of rat PFC based on morphine-induced conditioned place preference ( CPP) model was performed with two-dimensional gel electrophoresis ( 2-DE). Our results showed that a total of 80 proteins were differentially expressed by 2-DE analysis during three phases of CPP assay. Of them, 58 were further identified by mass spectrometry. These proteins were classified into multiple categories, such as energy metabolism, signal transduction, synaptic transmission, cytoskeletal proteins, chaperones, and local synaptic protein synthetic machinery according to their biological functions. Our study provides a global view of synaptic-related molecular networking in PFC under morphine-induced dependence, withdraw, and relapse, indicative of a concerted biological process in neuroadaptation under chronic morphine exposure.; Morphological alterations of synapse are found after morphine administration, suggesting that regulation of synaptic plasticity may be one of the mechanisms of neuroadaptation in addiction. However, the molecular basis underlying the abnormal synapse morphological and physiological changes in the morphine-induced dependence, withdraw, and relapse is not well understood. As prefrontal cortex ( PFC) is one of the most important brain regions, which provides executive control over drug use and is severely impaired in many addicts, systematic analysis of the biochemical and molecular alteration of synaptic fraction of PFC in morphine-induced neuroadaptation is necessary. In this study, differential protein expression profiling of synaptic fraction of rat PFC based on morphine-induced conditioned place preference ( CPP) model was performed with two-dimensional gel electrophoresis ( 2-DE). Our results showed that a total of 80 proteins were differentially expressed by 2-DE analysis during three phases of CPP assay. Of them, 58 were further identified by mass spectrometry. These proteins were classified into multiple categories, such as energy metabolism, signal transduction, synaptic transmission, cytoskeletal proteins, chaperones, and local synaptic protein synthetic machinery according to their biological functions. Our study provides a global view of synaptic-related molecular networking in PFC under morphine-induced dependence, withdraw, and relapse, indicative of a concerted biological process in neuroadaptation under chronic morphine exposure.
Keywordmorphine addiction conditioned place preference (CPP) prefrontal cortex (PFC) proteomics neuroplasticity
Subject Area医学心理学
Indexed BySCI
Language英语
WOS IDWOS:000246893500020
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/5639
Collection中国科学院心理研究所回溯数据库(1956-2010)
Corresponding AuthorY. P. Zhu
Affiliation1.Zhejiang Univ, Sch Med, Dept Toxicol, Hangzhou, Zhejiang, Peoples R China
2.Zhejiang Univ, James D Watson Inst Genome Sci, Hangzhou, Zhejiang, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Behav Genet Ctr, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Yang, Liu,Sun, Zhong Sheng,Zhu, Yong-ping,et al. Proteomic analysis of rat prefrontal cortex in three phases of morphine-induced conditioned place preference[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(6):2239-2247.
APA Yang, Liu,Sun, Zhong Sheng,Zhu, Yong-ping,&Y. P. Zhu.(2007).Proteomic analysis of rat prefrontal cortex in three phases of morphine-induced conditioned place preference.JOURNAL OF PROTEOME RESEARCH,6(6),2239-2247.
MLA Yang, Liu,et al."Proteomic analysis of rat prefrontal cortex in three phases of morphine-induced conditioned place preference".JOURNAL OF PROTEOME RESEARCH 6.6(2007):2239-2247.
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