Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress

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	Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress
	Zhang, Longze 1,2; Jie, Guoliang 3; Zhang, Junjing 1; Zhao, Baolu 1,2,4,5,6; B. L. Zhao
摘要	Epigallocatechin gallate (EGCG), a main active ingredient of green tea, is believed to be beneficial in association with anticarcinogenesis, antiobesity, and blood pressure reduction. Here we report that EGCG extended Caenorhabditis elegans longevity under stress. Under heat stress (35 degrees C), EGCG improved the meal) longevity by 13.1% at 0.1 mu g/ml, 8.0% at 1.0 mu g/ml, and 11.8% at 10.0 mu g/ml. Under oxidative stress, EGCG could improve the mean longevity of C. elegans by 172.9% at 0.1 mu g/ml, 177.7% at 1.0 mu g/ml, and 88.5% at 10.0 mu g/ml. However, EGCG could not extend the life span of C. elegans under normal culture conditions. Further studies demonstrated that the significant longevity-extending effects of EGCG on C elegans could be attributed to its in vitro and in vivo free radical-scavenging effects and its up-regulating effects on stress-resistance-related proteins, including superoxide dismutase-3 (SOD-3) and heat shock protein-16.2 (HSP-16.2), in transgenic C elegans with SOD-3: :green fluorescent protein (GFP) and HSP-16.2: :GFP expression. Quantitative real-time PCR results showed that the up-regulation of aging-associated genes such as daf-16, sod-3, and skn-1 could also contribute to the stress resistance attributed to EGCG. As the death rate of a population is closely related to the mortality caused by external stress, it could be concluded that the survival-enhancing effects of EGCG on C. elegans under stress are very important for antiaging research.; Epigallocatechin gallate (EGCG), a main active ingredient of green tea, is believed to be beneficial in association with anticarcinogenesis, antiobesity, and blood pressure reduction. Here we report that EGCG extended Caenorhabditis elegans longevity under stress. Under heat stress (35 degrees C), EGCG improved the meal) longevity by 13.1% at 0.1 mu g/ml, 8.0% at 1.0 mu g/ml, and 11.8% at 10.0 mu g/ml. Under oxidative stress, EGCG could improve the mean longevity of C. elegans by 172.9% at 0.1 mu g/ml, 177.7% at 1.0 mu g/ml, and 88.5% at 10.0 mu g/ml. However, EGCG could not extend the life span of C. elegans under normal culture conditions. Further studies demonstrated that the significant longevity-extending effects of EGCG on C elegans could be attributed to its in vitro and in vivo free radical-scavenging effects and its up-regulating effects on stress-resistance-related proteins, including superoxide dismutase-3 (SOD-3) and heat shock protein-16.2 (HSP-16.2), in transgenic C elegans with SOD-3: :green fluorescent protein (GFP) and HSP-16.2: :GFP expression. Quantitative real-time PCR results showed that the up-regulation of aging-associated genes such as daf-16, sod-3, and skn-1 could also contribute to the stress resistance attributed to EGCG. As the death rate of a population is closely related to the mortality caused by external stress, it could be concluded that the survival-enhancing effects of EGCG on C. elegans under stress are very important for antiaging research. (C) 2008 Elsevier Inc. All rights reserved.
关键词	EGCG ROS C. elegans Aging Antiaging Gene expression regulation Free radicals
学科领域	系统生物学/生物信息学
	2009-02-01
语种	英语
发表期刊	FREE RADICAL BIOLOGY AND MEDICINE
ISSN	0891-5849
卷号	46 期号:3 页码:414-421
期刊论文类型	Article
收录类别	SCI
WOS记录号	WOS:000262525300010
引用统计	被引频次：177[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/5685
专题	中国科学院心理研究所回溯数据库(1956-2010)
通讯作者	B. L. Zhao
作者单位	1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100029, Peoples R China 3.Zhejiang Univ, Hangzhou 310058, Zhejiang, Peoples R China 4.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Univ, Div Nitr Oxide & Inflammatory Med, Shanghai 201203, Peoples R China 5.Chinese Acad Sci, Inst Psychol, State Key Lab Mental Hlth, Beijing 100101, Peoples R China 6.E Inst Shanghai Municipal Educ Commiss, Shanghai 200003, Peoples R China
第一作者单位	脑与认知科学国家重点实验室
推荐引用方式 GB/T 7714	Zhang, Longze,Jie, Guoliang,Zhang, Junjing,et al. Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress[J]. FREE RADICAL BIOLOGY AND MEDICINE,2009,46(3):414-421.
APA	Zhang, Longze,Jie, Guoliang,Zhang, Junjing,Zhao, Baolu,&B. L. Zhao.(2009).Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress.FREE RADICAL BIOLOGY AND MEDICINE,46(3),414-421.
MLA	Zhang, Longze,et al."Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress".FREE RADICAL BIOLOGY AND MEDICINE 46.3(2009):414-421.

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