PSYCH OpenIR  > 中国科学院心理研究所回溯数据库(1956-2010)
Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress
Zhang, Longze1,2; Jie, Guoliang3; Zhang, Junjing1; Zhao, Baolu1,2,4,5,6; B. L. Zhao
2009-02-01
Source PublicationFREE RADICAL BIOLOGY AND MEDICINE
ISSN0891-5849
SubtypeArticle
Volume46Issue:3Pages:414-421
AbstractEpigallocatechin gallate (EGCG), a main active ingredient of green tea, is believed to be beneficial in association with anticarcinogenesis, antiobesity, and blood pressure reduction. Here we report that EGCG extended Caenorhabditis elegans longevity under stress. Under heat stress (35 degrees C), EGCG improved the meal) longevity by 13.1% at 0.1 mu g/ml, 8.0% at 1.0 mu g/ml, and 11.8% at 10.0 mu g/ml. Under oxidative stress, EGCG could improve the mean longevity of C. elegans by 172.9% at 0.1 mu g/ml, 177.7% at 1.0 mu g/ml, and 88.5% at 10.0 mu g/ml. However, EGCG could not extend the life span of C. elegans under normal culture conditions. Further studies demonstrated that the significant longevity-extending effects of EGCG on C elegans could be attributed to its in vitro and in vivo free radical-scavenging effects and its up-regulating effects on stress-resistance-related proteins, including superoxide dismutase-3 (SOD-3) and heat shock protein-16.2 (HSP-16.2), in transgenic C elegans with SOD-3: :green fluorescent protein (GFP) and HSP-16.2: :GFP expression. Quantitative real-time PCR results showed that the up-regulation of aging-associated genes such as daf-16, sod-3, and skn-1 could also contribute to the stress resistance attributed to EGCG. As the death rate of a population is closely related to the mortality caused by external stress, it could be concluded that the survival-enhancing effects of EGCG on C. elegans under stress are very important for antiaging research.; Epigallocatechin gallate (EGCG), a main active ingredient of green tea, is believed to be beneficial in association with anticarcinogenesis, antiobesity, and blood pressure reduction. Here we report that EGCG extended Caenorhabditis elegans longevity under stress. Under heat stress (35 degrees C), EGCG improved the meal) longevity by 13.1% at 0.1 mu g/ml, 8.0% at 1.0 mu g/ml, and 11.8% at 10.0 mu g/ml. Under oxidative stress, EGCG could improve the mean longevity of C. elegans by 172.9% at 0.1 mu g/ml, 177.7% at 1.0 mu g/ml, and 88.5% at 10.0 mu g/ml. However, EGCG could not extend the life span of C. elegans under normal culture conditions. Further studies demonstrated that the significant longevity-extending effects of EGCG on C elegans could be attributed to its in vitro and in vivo free radical-scavenging effects and its up-regulating effects on stress-resistance-related proteins, including superoxide dismutase-3 (SOD-3) and heat shock protein-16.2 (HSP-16.2), in transgenic C elegans with SOD-3: :green fluorescent protein (GFP) and HSP-16.2: :GFP expression. Quantitative real-time PCR results showed that the up-regulation of aging-associated genes such as daf-16, sod-3, and skn-1 could also contribute to the stress resistance attributed to EGCG. As the death rate of a population is closely related to the mortality caused by external stress, it could be concluded that the survival-enhancing effects of EGCG on C. elegans under stress are very important for antiaging research. (C) 2008 Elsevier Inc. All rights reserved.
KeywordEGCG ROS C. elegans Aging Antiaging Gene expression regulation Free radicals
Subject Area系统生物学/生物信息学
Indexed BySCI
Language英语
WOS IDWOS:000262525300010
Citation statistics
Cited Times:127[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/5685
Collection中国科学院心理研究所回溯数据库(1956-2010)
Corresponding AuthorB. L. Zhao
Affiliation1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100029, Peoples R China
3.Zhejiang Univ, Hangzhou 310058, Zhejiang, Peoples R China
4.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Univ, Div Nitr Oxide & Inflammatory Med, Shanghai 201203, Peoples R China
5.Chinese Acad Sci, Inst Psychol, State Key Lab Mental Hlth, Beijing 100101, Peoples R China
6.E Inst Shanghai Municipal Educ Commiss, Shanghai 200003, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Longze,Jie, Guoliang,Zhang, Junjing,et al. Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress[J]. FREE RADICAL BIOLOGY AND MEDICINE,2009,46(3):414-421.
APA Zhang, Longze,Jie, Guoliang,Zhang, Junjing,Zhao, Baolu,&B. L. Zhao.(2009).Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress.FREE RADICAL BIOLOGY AND MEDICINE,46(3),414-421.
MLA Zhang, Longze,et al."Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress".FREE RADICAL BIOLOGY AND MEDICINE 46.3(2009):414-421.
Files in This Item: Download All
File Name/Size DocType Version Access License
Zhang-2009-Significa(528KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Longze]'s Articles
[Jie, Guoliang]'s Articles
[Zhang, Junjing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Longze]'s Articles
[Jie, Guoliang]'s Articles
[Zhang, Junjing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Longze]'s Articles
[Jie, Guoliang]'s Articles
[Zhang, Junjing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Zhang-2009-Significant longevit.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.