Upregulation of PHLDA2 in Dicer knockdown HEK293 cells
Tang, Kai-Fu; Wang, Yan; Wang, Pengfei; Chen, Min; Chen, Yao; Hu, Huai-Dong; Hu, Peng; Wang, Bo; Yang, Wenjie; Ren, Hong; K. F. Tang; H. Ren
2007-05-01
发表期刊BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
ISSN0304-4165
文章类型Article
卷号1770期号:5页码:820-825
摘要It has been reported that RNAi-dependent chromatin silencing in vertebrates is not restricted to the centromeres. To address whether RNAi machinery could regulate the chromatin structure of imprinted genes, we knocked down Dicer in HEK293 cells and found that the expression of PHLDA2, one of the several genes in the imprinted gene domain of 11p15.5, was specifically upregulated. This was accompanied by a shift towards more activated chromatin at PHLDA2 locus as indicated by change in H3K9 acetylation, however, the methylation state at this locus was not affected. Furthermore, we found that PHLDA2 was downregulated in growth-arrested HEK293 cells induced by either serum deprivation or contact inhibition. This suggests that PHLDA2 upregulation might be a direct result of Dicer depletion rather than the consequence of growth arrest induced by Dicer knockdown. Considering the reports that there is consistent placental outgrowth in PHLDA2 knockout mice and that PHLDA2 overexpression in mice causes growth inhibition, we speculate that PHLDA2 may be a candidate for contributing to the reduced growth rate of Dicer-deficient cells and the very early embryonic lethality in Dicer knockout mice.; It has been reported that RNAi-dependent chromatin silencing in vertebrates is not restricted to the centromeres. To address whether RNAi machinery could regulate the chromatin structure of imprinted genes, we knocked down Dicer in HEK293 cells and found that the expression of PHLDA2, one of the several genes in the imprinted gene domain of 11p15.5, was specifically upregulated. This was accompanied by a shift towards more activated chromatin at PHLDA2 locus as indicated by change in H3K9 acetylation, however, the methylation state at this locus was not affected. Furthermore, we found that PHLDA2 was downregulated in growth-arrested HEK293 cells induced by either serum deprivation or contact inhibition. This suggests that PHLDA2 upregulation might be a direct result of Dicer depletion rather than the consequence of growth arrest induced by Dicer knockdown. Considering the reports that there is consistent placental outgrowth in PHLDA2 knockout mice and that PHLDA2 overexpression in mice causes growth inhibition, we speculate that PHLDA2 may be a candidate for contributing to the reduced growth rate of Dicer-deficient cells and the very early embryonic lethality in Dicer knockout mice. (c) 2007 Elsevier B.V. All rights reserved.
关键词PHLDA2 Dicer genomic imprinting embryonic lethality growth arrest
学科领域系统生物学/生物信息学
收录类别SCI
语种英语
WOS记录号WOS:000245832500011
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.psych.ac.cn/handle/311026/5827
专题中国科学院心理研究所回溯数据库(1956-2010)
通讯作者K. F. Tang; H. Ren
作者单位1.Chongqing Med Univ, Inst Viral Hepatitis, Affiliated Hosp 2, Key Lab Mol Biol Infect Dis,State Minist Educ, Chongqing 400010, Peoples R China
2.Chongqing Med Univ, Dept Emergency, Affiliated Hosp 2, Chongqing 400010, Peoples R China
3.Chongqing Med Univ, Dept Endocrinol, Affiliated Hosp 2, Chongqing 400010, Peoples R China
4.Chinese Acad Sci, Inst Psychol, Beijing 100101, Peoples R China
5.Tulane Univ, Sch Publ Hlth & Trop Med, Dept Epidemiol, New Orleans, LA 70112 USA
推荐引用方式
GB/T 7714
Tang, Kai-Fu,Wang, Yan,Wang, Pengfei,et al. Upregulation of PHLDA2 in Dicer knockdown HEK293 cells[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2007,1770(5):820-825.
APA Tang, Kai-Fu.,Wang, Yan.,Wang, Pengfei.,Chen, Min.,Chen, Yao.,...&H. Ren.(2007).Upregulation of PHLDA2 in Dicer knockdown HEK293 cells.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1770(5),820-825.
MLA Tang, Kai-Fu,et al."Upregulation of PHLDA2 in Dicer knockdown HEK293 cells".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1770.5(2007):820-825.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Tang-2007-Upregulati(291KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Tang, Kai-Fu]的文章
[Wang, Yan]的文章
[Wang, Pengfei]的文章
百度学术
百度学术中相似的文章
[Tang, Kai-Fu]的文章
[Wang, Yan]的文章
[Wang, Pengfei]的文章
必应学术
必应学术中相似的文章
[Tang, Kai-Fu]的文章
[Wang, Yan]的文章
[Wang, Pengfei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。