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Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment
Liang, Jing1; Chen, Ji-Huan2; Chen, Xiao-Hong3; Peng, Yong-Hua1; Zheng, Xi-Geng1; Zheng, XG (reprint author), Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China.
2011
Source PublicationBEHAVIOURAL BRAIN RESEARCH
ISSN0166-4328
SubtypeArticle
Volume216Issue:1Pages:321-331
Contribution Rank1
AbstractThe nucleus accumbens (NAc) is involved in contextual drug associations, which might be particularly important for environmental cue-induced relapse to drug seeking. In the present study, rats were first administered repeated morphine for 5 days (5 mg/kg, i.p.) in a contextually paired and unpaired design. After reexposure to the morphine-associated environment, which induced conditioned locomotor activity in the morphine-paired group, we performed a rat 27k 70-mer oligo array to profile gene expression in the NAc. One hundred fifty-five upregulated and 88 downregulated genes were found in the paired group compared with the unpaired group. Eight gene transcripts were then selected to confirm their alterations by quantitative real-time polymerase chain reaction (qRT-PCR). The identified genes generally play important roles in neuroactive receptor-ligand interactions, synapse plasticity, ion transport, and protein phosphorylation. Furthermore, the expression of the eight selected genes that were identified and confirmed to show significant fold changes in the first microarray experiment were again measured with qRT-PCR after morphine challenge (2 mg/kg, i.p.). As expected, 2 mg/kg morphine-induced context-specific sensitization. Meanwhile, mRNA expression of the selected genes showed marked upregulation in the morphine-paired group compared with the unpaired and acute groups. These results suggest that alterations in the expression of the identified genes in the NAc may contribute to the neuroplasticity underlying contextual cue-induced relapse to drug use. (C) 2010 Elsevier B.V. All rights reserved.
KeywordMorphine Conditioned response Sensitization Nucleus accumbens Microarray
Subject AreaCognitive Neuroscience
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Indexed BySCI
Language英语
Funding OrganizationNational Foundation of Natural Science [30470578, 30900400, 30770722] ; Institute of Psychology, Chinese Academy of Sciences [08CX053005]
Funding Project一室郑希耕课题组
Project Intro.This work was supported by the National Foundation of Natural Science (30470578, 30900400 and 30770722) and the Project for Young Scientists Fund, Institute of Psychology, Chinese Academy of Sciences (08CX053005). We would like to thank Michael Arends for editing the manuscript.
WOS IDWOS:000285318500044
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.psych.ac.cn/handle/311026/6917
Collection健康与遗传心理学研究室
Corresponding AuthorZheng, XG (reprint author), Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
2.Scripps Res Inst, Mol & Integrat Neurosci Dept, La Jolla, CA 92037 USA
3.CapitalBio Corp, Beijing 102206, Peoples R China
First Author AffilicationKey Laboratory of Mental Health, CAS
Recommended Citation
GB/T 7714
Liang, Jing,Chen, Ji-Huan,Chen, Xiao-Hong,et al. Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment[J]. BEHAVIOURAL BRAIN RESEARCH,2011,216(1):321-331.
APA Liang, Jing,Chen, Ji-Huan,Chen, Xiao-Hong,Peng, Yong-Hua,Zheng, Xi-Geng,&Zheng, XG .(2011).Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment.BEHAVIOURAL BRAIN RESEARCH,216(1),321-331.
MLA Liang, Jing,et al."Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment".BEHAVIOURAL BRAIN RESEARCH 216.1(2011):321-331.
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